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Abstract

The modulatory potentials of the ethanolic stem bark extract of Boswellia dalzieliiH. on diarrhea and gastrointestinal motility was investigated in rats. Antidiarrheal activity was evaluated in castor oil induced model using 25 inbreed, overnight fasted rats divided into five groups (A-E). Intestinal motility was determined using Charcoal transit time in 25 overnight fasted rats divided equally into five groups (A-E). Graded doses of the plant extract(100,200 and 400mg/kg respectively) were given to groups B, C and D, while group A served as vehicle control (VEH) (distilled water) and group E served as Atropine control(0.1mg/kg). Antispasmodic activity of the plant extract was determined with 3cm piece of isolated rat ileum incubated in a thermostatically regulated organ bath. The set-up allows for in vitro assessment of intestinal motility with atropine and acetylcholine as standard drugs for cholinergic muscarinic receptor potentials. The ethanolic stem bark extract of Boswellia dalzielli H significantly (P< 0.0001) and dose dependently reduced castor oil induced diarrhea and activated charcoal transit time in rats. The plant extract significantly (P<0.05) and dose dependently inhibited acetylcholine induced contractility of isolated ileac segments of rats when compared to controls. The ethanolic stem bark extract of Boswellia dalzielii H causes delay in gastrointestinal contractions via the inhibition of muscarinic cholinergic receptors in rats.

Keywords

Diarrhea, motility, intestinal, acetylcholine, Boswellia

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Antidiarrheal and Antispasmodic Effects of Ethanolic Stem Bark Extract of ‎Boswellia ‎dalzielii H. in Wistar Rats. (2023). Sahel Journal of Veterinary Sciences, 20(3), 21-27. https://doi.org/10.54058/saheljvs.v20i3.401
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How to Cite

Antidiarrheal and Antispasmodic Effects of Ethanolic Stem Bark Extract of ‎Boswellia ‎dalzielii H. in Wistar Rats. (2023). Sahel Journal of Veterinary Sciences, 20(3), 21-27. https://doi.org/10.54058/saheljvs.v20i3.401

References

  1. Akinsanmi, A. O., Johnson, O. T., Longdet, I. Y., and Aguiyi, ‎J.C. (2019). In vitro evaluation of free radical ‎scavenging, fe 2+ and snp-induced lipid ‎peroxidation (rat brain) activities of methanolic ‎extracts from three (3) northern Nigerian plants ‎leaf. Journal of Tropical Life Science, 9(1). ‎DOI:10.11594/JTLS.09.01.10‎
  2. Bakare R. I., Magbagbeola O. A., Akinwande A. I., ‎Okunowo, O. W. and Green M. (2011). ‎Antidiarrhoeal activity of aqueous leaf extract of ‎Momordica charantia in rats. Journal of ‎Pharmacognosy and Phytotherapy, 3(1), 1-7. ‎http://www.academicjournals.org/jpp
  3. Danlami, U., Daniel, G. J., David, B. M. and Galadanchi, ‎K.M. (2015). Phytochemical, nutritional and ‎antimicrobial screening of hexane, ethyl acetate ‎and ethanolic extracts of Boswellia dalzielii H. ‎leaves and bark. American Journal of Bioscience ‎and Bioengineering.‎ ‎3(576-79. ‎DOI:10.11648/j.bio.20150305.19‎
  4. Dandashire, B. S., Magashi, A.M., Abdulkadir, B., Abbas, ‎M.A., Goni, M.D. and Yakubu A. (2019) ‎Toxicological studies and bioactivity-guided ‎identification of antimicrobially active compounds ‎from crude aqueous stem bark extract ‎of Boswellia dalzielii. Journal of Advanced ‎Veterinary and ‎Animal Research.14;6(2):183 192.doi:10.5455/ja‎var.2019.f330. ‎
  5. Dubbelboer, I.R., Dahlgren, D., Sjögren, E. and Lennernäs, ‎H. (2019). Rat intestinal drug permeability: A ‎status report and summary of repeated ‎determinations. European Journal of ‎Pharmaceutics and Biopharmaceutics. doi: ‎‎10.1016/j.ejpb.2019.07.005‎
  6. Field, M. (2003). Intestinal ion transport and the ‎pathophysiology of diarrhea. Journal of Clinical ‎Investigation 111:931 943.doi:10.1172/JCI20031‎‎8326‎
  7. Gandhi, D. N. and Venkatakrishna-Bhatt, H. (1999). ‎Potentiation of acetylcholine-induced smooth ‎muscle contraction in rat ileum by lead. Annals of ‎the Academy of Medicine, Singapore, 28(4), 488- ‎‎493.PMID: 10561758‎
  8. Igboeli, N., Onyeto, C. A., Okorie, A. N., Mbaoji, F. N., ‎Nwabunike, I. A. and Alagboso, D. I. (2015). ‎Antidiarrheal activity of methanol leaf extract of ‎LophiralanceolataTiegh (Ochnaeceae). Merit ‎Research Journal of Environmental Science and ‎Toxicology. 3(4), 059-064. ‎http://www.meritresearchjournals.org/est/index.‎
  9. Jaafaru, M. S., Kyomson, I. D., Waziri, P. M., Yakubu, Y., ‎Mustapha, M. B., and Gyutorwa, J. S. (2017). In ‎vivo ameliorative effect of methanolic extract of ‎Boswellia dalzielli Hutch (Mebdh) stem bark on ‎Triton X-100 induced hyperlipidaemia. Science ‎World Journal. 12(4), 34-37. Vol 12(No 4) 2017. ‎www.scienceworldjournal.org
  10. Jespersen, B., Tykocki, N. R., Watts, S. W. and Cobbett, P. J. ‎‎(2015). Measurement of smooth muscle function ‎in the isolated tissue bath-applications to ‎pharmacology research. Journal of Visualized ‎Experiments. (95), e52324. DOI: 10.3791/52324.‎
  11. Kafuti, Y. S., Ojerinde, S. O., Balogun, O., Alemika, T. E., ‎Taba, K. M., Mpiana, P. T., and Kindombe, N. M. ‎‎(2017). Antifungal evaluation and phytochemical ‎screening of methanolic extract and fractions of ‎Boswellia dalzieliistem bark. Journal of Pharmacy ‎Bioresources.14 (1), 64-69. ‎https://dx.doi.org/10.4314/jpb.v14i1.8‎
  12. Kafuti, Y. S., Alemika, T. E., Ojerinde, S. O., Balogun, O., ‎Taba, K. M., Mpiana, P. T. and Kindombe, N. M. ‎‎(2018). Phytochemical studies, in vitro antioxidant ‎and antiproliferative of the stem bark of Boswellia ‎dalzielii hutch. IOJPH-International open GPH- ‎Journal of Advance Research in Applied Science. ‎‎1(3), 01-11. Article Text 130-1-10-20180914‎
  13. Kaur, M., Singh, A. and Kumar, B. (2014). Comparative ‎antidiarrheal and antiulcer effect of the aqueous ‎and ethanolic stem bark extracts of Tinospora ‎cordifolia in rats. Journal of Advanced ‎Pharmaceutical Technology and Research.5 (3), ‎‎122. DOI:10.4103/2231-4040.137417‎
  14. Kim, H. J. and Neophytou, C. (2009). Natural anti- ‎inflammatory compounds for the management ‎and adjuvant therapy of inflammatory bowel ‎disease and its drug delivery system. Archives of ‎pharmacal Research, 32(7), 997-1004. DOI: ‎‎10.1007/s12272-009-1704-1. ‎
  15. ‎ Kima, K., Shimb, W., Peñac, I.C.D.,Seod , E., Kima ,W. ‎Jina, H., Kima, D., Chunga, S., Cheongc, J. and ‎Shima, C. (2013). Smooth muscle relaxation ‎activity of an aqueous extract of dried immature ‎fruit of poncirus trifoliata (PF-W) on an isolated ‎strip of rat ileum. Natural Product ‎Communications. 8 (8): 1143 – 1148 ‎https://doi.org/10.1177/1934578X1300800829‎
  16. Lee, J. A., Qu, K., Gainey, M., Kanekar, S. S., Barry, M. A., ‎Nasrin, S., and Levine, A. C. (2021). Continuous ‎diagnostic models for volume deficit in patients ‎with acute diarrhea. Tropical Medicine and Health. ‎‎49, 1-11. https://doi.org/10.1186/s41182-021-‎‎00361-9‎
  17. Nwinyi, F. C., Binda, L., Ajoku, G. A., Aniagu, S. O., ‎Enwerem, N. M., Orisandipe, A. and Gamaniel, K. ‎S. (2004). Evaluation of the aqueous extract of ‎Boswellia dalzielii stem bark for ‎antimicrobial activities and gastrointestinal effects. ‎African Journal of Biotechnology. 3(5), 284-‎‎288.http://www.academicjournals.org/AJB
  18. Palombo, E. A. (2006). Phytochemicals from traditional ‎medicinal plants used in the treatment of ‎diarrhoea: modes of action and effects on ‎intestinal function. Phytotherapy Research. 20(9), ‎‎717-724.DOI: 10.1002/ptr.1907‎