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Abstract
Despite mounting evidence that small ruminants have a role in disease transmission, the epidemiological status for foot-and-mouth disease (FMD) in Nigeria is inadequate and under-reported. In this study, we looked at the seroprevalence of foot and mouth disease virus in goat species in Port Harcourt, River State. A total of 356 blood samples from goats were obtained during slaughter at seven different abattoirs in Port Harcourt, Rivers State. Samples were processed and analysed for FMD using an in-house 3ABC competitive Enzyme-Linked Immunosorbent Assay (ELISA), as well as serotypes A, O, SAT 1, and SAT 2 specific ELISA. An overall seroprevalence of 37.1% (32.21 - 42.1) for FMD was recorded. The seroprevalence based on location revealed a higher prevalence in Emenike 85.2% (Cl: 73.8 - 92.9), followed by Ruememe 60% (CI: 46.4 - 73.6), Mile3 54.0% (Cl: 41.6 - 66.0), Aluu 42% (Cl: 28.2 - 56.0), Rumokoro 38.7% (Cl: 26.0 - 52.9), Choba 29.3% (Cl: 16.9 - 44.5) respectively while, Rumosi had the least seroprevalence of 8.2% (Cl: 2.6-18.5). This study also detected the circulation of FMD virus serotype O. The study demonstrates high seroprevalence of FMD in goats. Thus, a comprehensive surveillance and vaccination campaign is required to check the silent amplification and transmission of the virus to other animals
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References
- Abah, A. E., and Udoidang, I. N. (2019). Co-infection of malaria and hepatitis B virus in Port Harcourt, Rivers State, Nigeria. International Journal of Infection, 6(4), e97033.
- Alexandersen, S., Zhang, Z., and Donaldson, A. I. (2002). Aspects of the persistence of foot-and-mouth disease virus in animals the carrier problem. Microbes and Infection, 4(10), 1099-1110.
- Arzt, J; Juleff, N.; Zhang, Z. and Rodriguez, L.L. (2011). The pathogenesis of Foot and Mouth Disease. I; Viral pathways in cattle. Transboundary and Emerging Diseases, 58(4): 291-304.
- Aslam, M., andAlkheraije, K. A. (2023). The prevalence of foot-and-mouth disease in Asia. Frontiers in Veterinary Science, 10, 1201578. https://doi.org/10.3389/fvets.2023.1201578.
- Balinda, S. N., Tjørnehøj, K., Muwanika, V. B., Sangula, A. K., Mwiine, F. N., Ayebazibwe, C., Masembe, C., Siegismund, H. R., and Alexandersen, S. (2009). Prevalence estimates of antibodies towards foot-and-mouth disease virus in small ruminants in Uganda. Transboundary and Emerging Diseases, 56(9-10), 362–371. https://doi.org/10.1111/j.1865-1682.2009.01094.x
- Barnett, P.V. and Cox, S.J. (1999). The role of small ruminants in the epidemiology and transmission of foot-and-mouth disease. Veterinary Journal; 158: 6–13.
- Bayissa, B., Ayelet, G., Kyule, M., Jibril, Y. and Gelaye, E. (2011). Study on seroprevalence, risk factors, and economic impact of foot-and-mouth disease in Borena pastoral and agro-pastoral system, southern Ethiopia. Tropical Animal Health and Production. 43, 759–766.
- Begovoeva, M., Ehizibolo, D. O., Adedeji, A. J., Oguche, M. O., Oyekan, O., Ijoma, S. I., Atai, R. B., Wungak, Y., Dogonyaro, B. B., Lazarus, D. D., Samson, M., Ularamu, H., Muhammad, M., Rosso, F., Sumption, K. J., Beard, P. M., Ludi, A. B., Stevens, K. B., & Limon, G. (2023). Factors associated with foot-and-mouth disease seroprevalence in small ruminants and identification of hot-spot areas in northern Nigeria. Preventive Veterinary Medicine, 212, 105842. https://doi.org/10.1016/j.prevetmed.2023.105842
- Brocchi, E., Bergmann, I. E., Dekker, A., Paton, D. J., Sammin, D. J., Greiner, M., Grazioli, S., De Simone, F., Yadin, H., Haas, B., Bulut, N., Malirat, V., Neizert, E., Goris, N., Parida, S., Sǿrensen, K. and De Clercq, K. (2006). Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus. Vaccine, 24: 6966-79.
- Chukwuedo. A. A., Nimzing, L., Olabode, A.O. and Abegunde, A. (2008). Viral Diseases of animals in Africa. OAU/STRC Prevalence of Foot and Mouth Disease Virus, SAT1 and SAT2 serotype antibodies in Nigerian cattles. Animal Production Research Advances. 4 (2): 157-160
- Clavijo, A., Wright, P., and Kitching, P. (2004). Developments in diagnostic techniques for differentiating infection from vaccination in foot-and-mouth disease. Veterinary Journal (London, England: 1997), 167(1), 9–22. https://doi.org/10.1016/s1090-0233 (03)00087-x
- Dean, A. G., Sullivan, K. M., & Soe, M. M. (2013). OpenEpi: Open source epidemiologic statistics for public health (Version 3.01) [Computer software]. https://www.openepi.com
- (Accessed April 25, 2020)
- Fasina, F.O., Connell, D.R., Talabi, O.A., Lazarus, D.D. and Adeleke, G.A. (2013). Foot-and-mouth disease virus strains and examination of exposure factors associated with seropositivity of cattle herds in Nigeria during 2007-2009. Preventive Veterinary Medicine., 109: 334-342.
- Fry, E. E., Stuart, D. I. and Rowlands, D. J. (2005). “The structure of foot-and-mouth disease virus,” Current Topics in Microbiology and Immunology, vol. 288, no. 288, pp. 71–101.
- Ganter, M.; Graunke, W.D.; Steng, G. and Worbes, H. (2001): FMD in sheep and goats. Dtsch Tierarztl Wochenschr, 108 (12): 499-503
- Gubbins, S., Brown, E., Wungak, Y., Oyekan, O., Adedeji, A. J., Ijoma, S. I. and Ehizibolo, D. O. (2025). Longitudinal study of foot-and-mouth disease virus in Northern Nigeria: implications for the roles of small ruminants and environmental contamination in endemic settings. Veterinary Research, 56(1), 76.
- Hamond, J. (2011). FMD Vaccine: Practical Applications from an International Perspective-FMDV Vaccine to Live. An event organised by NFUS, Moredun and Scottish Government, 15 March 2011.
- James, A.D. and Rushton, J. (2002). The economics of foot and mouth disease. Revue Scientifique et Technique (OIE) 21, 637–644.
- Jumbo, S., Noumedem, R., Wade, A., Dah, I., God-Yang, L., lendzele, S., Mbanwi, C., Niba, A., and Awah-Ndukum, J. (2024). Seroprevalence and risk factors of foot-and-mouth disease in sheep and goats in the Soudan-Sahelian and Guinean regions of Cameroon. Microbes and Infectious Diseases, doi: 10.21608/mid.2024.256723.1724
- Kitching, R.P. and Hughes, G.J. (2002). Clinical variation in foot and mouth disease: sheep and goats. Rev Sci Tech; 21:505–512.
- Knowles, N.J. and Samuel, A.R. (2003). Molecular epidemiology of foot-and-mouth disease virus. Virus Res; 91(1):65-80.
- Lazarus, D. D., Schielen, W. J. G., Wungak, Y. and Kwange, D. N.D.G. (2012a) Foot and Mouth disease in Nigeria: The current status and control efforts. A paper presented at the Global Foot and Mouth Disease Research Alliance workshop organized by ARC-Onderstepoort Veterinary Institute, held at Hazy-view, Kurger National Park, South Africa on the 17th-19th April, 2012.
- Lazarus, D. D., Schielen, W. J. G., Wungak, Y., Kwange, D., and Fasina, F. O (2012b). Foot and Mouth disease in Nigeria: The Sero-epidemiology of foot-and-current status and control efforts. A Paper presented at the mouth disease in some Border States of Nigeria. Afr. J. Global Foot and Mouth Disease Research Alliance Microbiology Research, 6 (8): 1756-1761.
- Lazarus, D.D., Wungak, Y.S. and Adah. M.I. (2015). Serological response of commercial dairy cattle to inactivated foot-and-mouth disease vaccine (type-O&A) in Nigeria. Asian journal of Medical and Biological Research; 1(2): 163-168
- Mansley, L.M., Donaldson, A.I., Thrusfield, M.V. and Honhold, N. (2011). Destructive tension: mathematics versus experience – the progress and control of the 2001 foot and mouth disease epidemic. Revue Scientifique et Technique (OIE) 30, 483–498.
- Ogiji, E., Ibu, J., Akwuobu, C., Ularamu, H., & Rabo, N. (2025). Spatial seroprevalence of foot-and-mouth disease in small ruminants in Benue State, Nigeria. Veterinaria Italiana, 61(1). https://doi.org/10.xxxx/vetital.xxxxxx
- OIE. (2004). Manual of diagnostic tests and vaccines for testerial animals (mammals, birds, bees). 5nd ed. OIE, Paris, France pp. 111-128.
- OIE. (2009). Foot-and-Mouth Disease, Chapter 2.1.5. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees), Web Edition.
- Olabode, H.O.K., Kazeem, H. M., Raji, M.A., Ibrahim, and Fasina, F. O. (2012) Sero-epidemiology of foot-and- forts. A Paper presented at the workshop organized by ARC-Onderstepoort. Veterinary Microbiology Research, 6 (8): 1756-1761.
- Olabode, O., Kazeem, H., Raji, M. and Ibrahim, N. (2013). Seroprevalence of foot and mouth disease virus antibodies in trade cattle (Bos indicus) in Kwara state of Nigeria, Veterinary World, vol. 6, no. 10, pp. 1–9.
- Onono, J. O., Wieland, B., and Rushton, J. (2013). Constraints to cattle production in a semiarid pastoral system in Kenya. Tropical Animal Health and production, 45(6), 1415-1422.
- Paton, D.J., de Clercq, K., Greiner, M., Dekker, A., Brocchi, E., Bergmann, I., Sammin, D.J., Gubbins, S. and Parida, S. (2006). Application of non-structural protein antibody tests in substantiating freedom from foot-and-mouth disease virus infection after emergency vaccination of cattle. Vaccine 24, 6503–6512.
- Ploquin, O., Grosbois, V., Ndlovu, M., Ndozore, S., Munzamba, M., Porovha, E., Nkomo, K., Basso, O., Corbel, G., Shumba, R. …and Miguel, E. (2025). Foot-and-mouth disease dynamics in multi-species livestock systems at the interface of African protected areas. Veterinary Research, 56(1), 58. https://doi.org/10.1186/s13567-025-01487-y
- Rweyemamu, M., Roeder, P., Mackay, D., Sumption, K., Brownlie, J., Leforban, Y., Valarcher, J. F., Knowles, N. J., and Saraiva, V. (2008). Epidemiological patterns of foot-and-mouth disease worldwide. Transboundary and Emerging Diseases, 55(1), 57–72. https://doi.org/10.1111/j.1865-1682.2007.01013.x
- Shao, J., Hui-Yun, C., Guang-Qing, Z. and Guo-Zheng, C. (2010). Jun- enzyme-linked immunosorbent assay in comparison with Zheng, D., Tong, L., Shan-Dian, G., Ji-Jun, H., Xiang-Tao, complement fixation for the diagnosis of foot-and-mouth L., Ji-Xing, L., and Jin-Liang, G. (2010) RT Rapid Detection and swine vesicular disease. Vet. Microbiol 16: 201–209.
- Ullah, M., Li, Y., Munib, K., Rahman, H. U., and Zhang, Z. (2023). Sero-Epidemiology and Associated Risk Factors of Foot-and-Mouth Disease (FMD) in the Northern Border Regions of Pakistan. Veterinary Sciences, 10(5), 356. https://doi.org/10.3390/vetsci10050356
- Wungak, Y. S., Ishola, O. O., Olugasa, B. O., Lazarus, D. D., Ehizibolo, D. O., and Ularamu, H. G. (2017). Spatial pattern of foot-and-mouth disease virus serotypes in North Central Nigeria. Veterinary World, 10(4), 450.
- Wungak, Y. S., Olugasa, B. O., Ishola, O. O., Lazarus, D. D. and Ularamu, G. H. (2016). Foot-and-mouth disease (FMD) prevalence and exposure factors associated with seropositivity of cattle in north-central, Nigeria. African Journal of Biotechnology, 15(24), 1224-1232.
References
Abah, A. E., and Udoidang, I. N. (2019). Co-infection of malaria and hepatitis B virus in Port Harcourt, Rivers State, Nigeria. International Journal of Infection, 6(4), e97033.
Alexandersen, S., Zhang, Z., and Donaldson, A. I. (2002). Aspects of the persistence of foot-and-mouth disease virus in animals the carrier problem. Microbes and Infection, 4(10), 1099-1110.
Arzt, J; Juleff, N.; Zhang, Z. and Rodriguez, L.L. (2011). The pathogenesis of Foot and Mouth Disease. I; Viral pathways in cattle. Transboundary and Emerging Diseases, 58(4): 291-304.
Aslam, M., andAlkheraije, K. A. (2023). The prevalence of foot-and-mouth disease in Asia. Frontiers in Veterinary Science, 10, 1201578. https://doi.org/10.3389/fvets.2023.1201578.
Balinda, S. N., Tjørnehøj, K., Muwanika, V. B., Sangula, A. K., Mwiine, F. N., Ayebazibwe, C., Masembe, C., Siegismund, H. R., and Alexandersen, S. (2009). Prevalence estimates of antibodies towards foot-and-mouth disease virus in small ruminants in Uganda. Transboundary and Emerging Diseases, 56(9-10), 362–371. https://doi.org/10.1111/j.1865-1682.2009.01094.x
Barnett, P.V. and Cox, S.J. (1999). The role of small ruminants in the epidemiology and transmission of foot-and-mouth disease. Veterinary Journal; 158: 6–13.
Bayissa, B., Ayelet, G., Kyule, M., Jibril, Y. and Gelaye, E. (2011). Study on seroprevalence, risk factors, and economic impact of foot-and-mouth disease in Borena pastoral and agro-pastoral system, southern Ethiopia. Tropical Animal Health and Production. 43, 759–766.
Begovoeva, M., Ehizibolo, D. O., Adedeji, A. J., Oguche, M. O., Oyekan, O., Ijoma, S. I., Atai, R. B., Wungak, Y., Dogonyaro, B. B., Lazarus, D. D., Samson, M., Ularamu, H., Muhammad, M., Rosso, F., Sumption, K. J., Beard, P. M., Ludi, A. B., Stevens, K. B., & Limon, G. (2023). Factors associated with foot-and-mouth disease seroprevalence in small ruminants and identification of hot-spot areas in northern Nigeria. Preventive Veterinary Medicine, 212, 105842. https://doi.org/10.1016/j.prevetmed.2023.105842
Brocchi, E., Bergmann, I. E., Dekker, A., Paton, D. J., Sammin, D. J., Greiner, M., Grazioli, S., De Simone, F., Yadin, H., Haas, B., Bulut, N., Malirat, V., Neizert, E., Goris, N., Parida, S., Sǿrensen, K. and De Clercq, K. (2006). Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus. Vaccine, 24: 6966-79.
Chukwuedo. A. A., Nimzing, L., Olabode, A.O. and Abegunde, A. (2008). Viral Diseases of animals in Africa. OAU/STRC Prevalence of Foot and Mouth Disease Virus, SAT1 and SAT2 serotype antibodies in Nigerian cattles. Animal Production Research Advances. 4 (2): 157-160
Clavijo, A., Wright, P., and Kitching, P. (2004). Developments in diagnostic techniques for differentiating infection from vaccination in foot-and-mouth disease. Veterinary Journal (London, England: 1997), 167(1), 9–22. https://doi.org/10.1016/s1090-0233 (03)00087-x
Dean, A. G., Sullivan, K. M., & Soe, M. M. (2013). OpenEpi: Open source epidemiologic statistics for public health (Version 3.01) [Computer software]. https://www.openepi.com
(Accessed April 25, 2020)
Fasina, F.O., Connell, D.R., Talabi, O.A., Lazarus, D.D. and Adeleke, G.A. (2013). Foot-and-mouth disease virus strains and examination of exposure factors associated with seropositivity of cattle herds in Nigeria during 2007-2009. Preventive Veterinary Medicine., 109: 334-342.
Fry, E. E., Stuart, D. I. and Rowlands, D. J. (2005). “The structure of foot-and-mouth disease virus,” Current Topics in Microbiology and Immunology, vol. 288, no. 288, pp. 71–101.
Ganter, M.; Graunke, W.D.; Steng, G. and Worbes, H. (2001): FMD in sheep and goats. Dtsch Tierarztl Wochenschr, 108 (12): 499-503
Gubbins, S., Brown, E., Wungak, Y., Oyekan, O., Adedeji, A. J., Ijoma, S. I. and Ehizibolo, D. O. (2025). Longitudinal study of foot-and-mouth disease virus in Northern Nigeria: implications for the roles of small ruminants and environmental contamination in endemic settings. Veterinary Research, 56(1), 76.
Hamond, J. (2011). FMD Vaccine: Practical Applications from an International Perspective-FMDV Vaccine to Live. An event organised by NFUS, Moredun and Scottish Government, 15 March 2011.
James, A.D. and Rushton, J. (2002). The economics of foot and mouth disease. Revue Scientifique et Technique (OIE) 21, 637–644.
Jumbo, S., Noumedem, R., Wade, A., Dah, I., God-Yang, L., lendzele, S., Mbanwi, C., Niba, A., and Awah-Ndukum, J. (2024). Seroprevalence and risk factors of foot-and-mouth disease in sheep and goats in the Soudan-Sahelian and Guinean regions of Cameroon. Microbes and Infectious Diseases, doi: 10.21608/mid.2024.256723.1724
Kitching, R.P. and Hughes, G.J. (2002). Clinical variation in foot and mouth disease: sheep and goats. Rev Sci Tech; 21:505–512.
Knowles, N.J. and Samuel, A.R. (2003). Molecular epidemiology of foot-and-mouth disease virus. Virus Res; 91(1):65-80.
Lazarus, D. D., Schielen, W. J. G., Wungak, Y. and Kwange, D. N.D.G. (2012a) Foot and Mouth disease in Nigeria: The current status and control efforts. A paper presented at the Global Foot and Mouth Disease Research Alliance workshop organized by ARC-Onderstepoort Veterinary Institute, held at Hazy-view, Kurger National Park, South Africa on the 17th-19th April, 2012.
Lazarus, D. D., Schielen, W. J. G., Wungak, Y., Kwange, D., and Fasina, F. O (2012b). Foot and Mouth disease in Nigeria: The Sero-epidemiology of foot-and-current status and control efforts. A Paper presented at the mouth disease in some Border States of Nigeria. Afr. J. Global Foot and Mouth Disease Research Alliance Microbiology Research, 6 (8): 1756-1761.
Lazarus, D.D., Wungak, Y.S. and Adah. M.I. (2015). Serological response of commercial dairy cattle to inactivated foot-and-mouth disease vaccine (type-O&A) in Nigeria. Asian journal of Medical and Biological Research; 1(2): 163-168
Mansley, L.M., Donaldson, A.I., Thrusfield, M.V. and Honhold, N. (2011). Destructive tension: mathematics versus experience – the progress and control of the 2001 foot and mouth disease epidemic. Revue Scientifique et Technique (OIE) 30, 483–498.
Ogiji, E., Ibu, J., Akwuobu, C., Ularamu, H., & Rabo, N. (2025). Spatial seroprevalence of foot-and-mouth disease in small ruminants in Benue State, Nigeria. Veterinaria Italiana, 61(1). https://doi.org/10.xxxx/vetital.xxxxxx
OIE. (2004). Manual of diagnostic tests and vaccines for testerial animals (mammals, birds, bees). 5nd ed. OIE, Paris, France pp. 111-128.
OIE. (2009). Foot-and-Mouth Disease, Chapter 2.1.5. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees), Web Edition.
Olabode, H.O.K., Kazeem, H. M., Raji, M.A., Ibrahim, and Fasina, F. O. (2012) Sero-epidemiology of foot-and- forts. A Paper presented at the workshop organized by ARC-Onderstepoort. Veterinary Microbiology Research, 6 (8): 1756-1761.
Olabode, O., Kazeem, H., Raji, M. and Ibrahim, N. (2013). Seroprevalence of foot and mouth disease virus antibodies in trade cattle (Bos indicus) in Kwara state of Nigeria, Veterinary World, vol. 6, no. 10, pp. 1–9.
Onono, J. O., Wieland, B., and Rushton, J. (2013). Constraints to cattle production in a semiarid pastoral system in Kenya. Tropical Animal Health and production, 45(6), 1415-1422.
Paton, D.J., de Clercq, K., Greiner, M., Dekker, A., Brocchi, E., Bergmann, I., Sammin, D.J., Gubbins, S. and Parida, S. (2006). Application of non-structural protein antibody tests in substantiating freedom from foot-and-mouth disease virus infection after emergency vaccination of cattle. Vaccine 24, 6503–6512.
Ploquin, O., Grosbois, V., Ndlovu, M., Ndozore, S., Munzamba, M., Porovha, E., Nkomo, K., Basso, O., Corbel, G., Shumba, R. …and Miguel, E. (2025). Foot-and-mouth disease dynamics in multi-species livestock systems at the interface of African protected areas. Veterinary Research, 56(1), 58. https://doi.org/10.1186/s13567-025-01487-y
Rweyemamu, M., Roeder, P., Mackay, D., Sumption, K., Brownlie, J., Leforban, Y., Valarcher, J. F., Knowles, N. J., and Saraiva, V. (2008). Epidemiological patterns of foot-and-mouth disease worldwide. Transboundary and Emerging Diseases, 55(1), 57–72. https://doi.org/10.1111/j.1865-1682.2007.01013.x
Shao, J., Hui-Yun, C., Guang-Qing, Z. and Guo-Zheng, C. (2010). Jun- enzyme-linked immunosorbent assay in comparison with Zheng, D., Tong, L., Shan-Dian, G., Ji-Jun, H., Xiang-Tao, complement fixation for the diagnosis of foot-and-mouth L., Ji-Xing, L., and Jin-Liang, G. (2010) RT Rapid Detection and swine vesicular disease. Vet. Microbiol 16: 201–209.
Ullah, M., Li, Y., Munib, K., Rahman, H. U., and Zhang, Z. (2023). Sero-Epidemiology and Associated Risk Factors of Foot-and-Mouth Disease (FMD) in the Northern Border Regions of Pakistan. Veterinary Sciences, 10(5), 356. https://doi.org/10.3390/vetsci10050356
Wungak, Y. S., Ishola, O. O., Olugasa, B. O., Lazarus, D. D., Ehizibolo, D. O., and Ularamu, H. G. (2017). Spatial pattern of foot-and-mouth disease virus serotypes in North Central Nigeria. Veterinary World, 10(4), 450.
Wungak, Y. S., Olugasa, B. O., Ishola, O. O., Lazarus, D. D. and Ularamu, G. H. (2016). Foot-and-mouth disease (FMD) prevalence and exposure factors associated with seropositivity of cattle in north-central, Nigeria. African Journal of Biotechnology, 15(24), 1224-1232.
