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Abstract
An eleven-year-old Dachshund cross-bred dog was presented to the Small Animal Clinic of Ahmadu Bello University Veterinary Teaching Hospital, Zaria, with complaints of reduced appetite noticed 2 days earlier, and an enlarged testis was noticed three months prior to presentation. Clinical diagnosis was based on history of reduced appetite and enlarged testis; physical findings such as pyrexia, tick infestation, congested ocular mucous membranes, generalized enlarged superficial lymph nodes, enlargement of the mammary glands, alopecia, atrophy of the right testis and discoloration of the scrotum with large, firm palpable mass; complete blood count revealed slight anaemia (PCV 34 %, neutrophilia (12.88 x 109/L) and presence of band cells (0.32 x 109/L); buffy coat smear showed the presence of intracytoplasmic morulae in just one monocyte in the peripheral blood smear; Ehrlichia canis antibodies was detected in serum using Ehrlichia test kit (immunochromatography); serum oestradiol-17β (42.05 pg/mL) and anti- Müllerian hormone (AMH; 25.50 μg/mL) were employed as serum tumour markers; transverse and longitudinal scrotal sonographs revealed diffuse non-homogenous tissues with increase in the echogenicity of the testicles; radiographic findings of thoraco-abdomino-pelvic regions revealed normal organ opacity indicating absence of metastases. Tissue samples were taken from the tumor following surgical excision and submitted for histopathology. The findings were large cytoplasmic vacuoles with slightly irregular nucleoli. It was confirmed to be sertoli cell tumor. The conditions were managed medically, and by surgical excision of the tumour and scrotal ablation. Outcome was successful, and patient started feeding 24 hours after the surgery.
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- Banco, B., Veronesi, M.C, Giudice, C., Rota, A., Grieco, V. (2012). Immunohistochemical evaluation of the expression of Anti-Müllerian Hormone in mature, immature and neoplastic Canine Sertoli cells. J. Comp. Path., 146(1): 18-23
- Cahua, U.J., Sandoval, C.N., Fernández, A.V. Puicón, N.V. (2015). Pyometra and Sertoli cell tumor in a dog with persistent Müller duct. Rev. Invest. Vet. Perú (RIVEP), 26(4): 705-711
- Carreira, V.S., Langohr, I.M., Ferrari, H.F., RuiLuvizotto, M.C. (2012). Pathology in practice: Sertoli cell tumor (SCT). J. Am. Vet. Med. Assoc., 241(1): 55-57
- Castro, J.L.C., Albernaz, V.G.P., Domingues, C.M.T., Ferreira, A.A., Dittrich, G., Moratelli, C.B.S., Freitag, F.V. Miara, L.C. (2016). Malignant Sertoli cell tumor with abdominal infiltrative metastasis in a dog. Pak. Vet. J., 36(3): 379-381
- Dhliwayo, S., Chihambakwe, B., Taonezvi, K., Chikerema, S.M., Tivapasi, M.T. and Pfukenyi, D.M. (2019). Seroprevalence of Canine Ehrlichiosis and Microscopic Screening for Canine Babesiosis in Dogs in Harare, Zimbabwe, 2016-2017. Vet. Med. Int., Article ID 4130210, https://doi.org/10.1155/2019/4130210
- Ferguson, L. and Agoulnik, A.I. (2013). Testicular cancer and cryptorchidism. Front. Endocrinol., 4 (32): 1-9
- Fourie, J.J., Horak, I., Crafford, D., Erasmus, H.L., Botha, O.J. (2015). The efficacy of a generic doxycycline tablet in the treatment of Canine monocyticEhrlichiosis. J. S. Afr. Vet. Assoc., 86:1193
- Gaunt, S., Beall, M., Stillman, B., Lorentzen, L., Diniz, P., Chandrashekar, R. Breitschwerdt, E. (2010). Experimental infection and co-infection of dogs with Anaplasmaplatys and Ehrlichiacanis: hematologic, serologic and molecular findings. Para. &Vect., 3 (1): 33
- Grieco, V., Riccardi, E., Greppi, G.F., Teruzzi, F., Iermano, V., Finazzi, M. (2008). Canine testicular tumours: a study on 232 dogs. J. Comp. Pathol., 138(2): 86–89
- Harrus, S., Warner, T., Neer, M. (2012). Ehrlichiacanis infection. In: Sykes, J. and Greene, C., (Eds.) Infectious Diseases of the Dog and Cat. Elsevier Saunders, St. Louis, Missouri, pp. 227-238
- Harrus, S. and Waner, T. (2011). Diagnosis of Canine monocytotropicEhrlichiosis (Ehrlichiacanis): an overview. Vet. J., 187: 292-296
- Herndon, A.M., Casal, M.L., Jaques, J.T.S. (2012). Testicular neoplasia in the retained testicles of an intersex male dog. J. Am. Anim. Hosp. Assoc., 48(2): 118-124
- Hohsteter, M., Artuskovic, M., Severin, K., Kurilj, A.G., Beck, A., Sostaric-Zuckermann, I.C., Grabarevic, Z. (2014). Canine testicular tumors: two types of seminomas can be differentiated by immunohistochemistry. Vet. Res., 10: 1-9
- Holst, B.S. and Dreimanis, U. (2015). Anti-Müllerian hormone: a potentially useful biomarker for the diagnosis of canine Sertoli cell tumours. BMC Vet. Res., 11: 166-172
- Hornakova, T., Vrbovska, M., Pavlak, A., Valencakova-Agyagosova, M., Halo, J. H. (2017). The evaluation of blood concentrations of testosterone, 17β-oestradiol and anti-Mullerian hormone in dogs with cryptorchidism and testicular tumours. Pol. J. Vet. Sci., 20(4): 677-685
- Jain, N.C. (1986). Schalm’s Veterinary Haematology. 4th Edition, Lea and Febiger, Philadelphia, U.S.A., pp. 58-90
- Kaewmongkol, G., Maneesaay, P., Suwanna, N., Tiraphut, B., Krajarngjang, T., Chouybumrung, A., Kaewmongkol, S., Sirinarumitr, T., Jittapalapong, S., Fenwick, S.G. (2016). First Detection of Ehrlichiacanis in cerebrospinal fluid from a non-thrombocytopenic dog with meningo-encephalitis by broad-range PCR. J. Vet. Intern. Med., 30(1): 255-259
- Kang, S.C., Yang, H.S., Jung, J.Y., Jung, E.H., Lee, H.C. (2011). Malignant Sertoli cell tumor in Shih Tzu dog. Korean J. Vet. Res., 51:171-175
- Kottadamane, M.R., Dhaliwal, P.S. and Singla, L.D. (2016). Diagnosis and treatment of canine monocyticehrlichiosis in a boxer breed of dog – a case report. Intern. J. Sci. Envt. Tech., 5(5): 3099 – 3105
- Kukreti, K., Das, M.K., Rastogi, A.K., Dubey, R., Pandey, L.K. and Sharma, P. (2018). Prevalence of Canine MonocyticEhrlichiosis in Canine Population Across India. Arch. Razi Inst., 73(2): 87-93
- Lawrence, J.A. and Saba, C.F. (2013). Tumors of the male Reproductive system. In: Withrow and MacEwen (Eds.) Small Animal Clinical Oncology. Elsevier Saunders, USA, pp. 557–571
- Liao, A.T., Chu, P.Y., Yeh, L.S., Lin, C.T., Liu, C.H. (2009). A 12-year retrospective study of canine testicular tumors. J. Vet. Med. Sci., 71(7): 919–923
- Mohamed, S., Bauchet, A-L., Fiette, L. (2017). Tissue sampling and processing for histopathology evaluation. Histopathology procedures. In: Gautier, J-C. (Ed.) Drug Safety Evaluation: methods and protocols, methods in molecular biology. Springer Science+Business Media LLC, pp. 101-114
- Mylonakis, M.E., Koutinas, A.F., Breitscwerdt, E.B., Hegarty, B.C., Billinis, C.D., Leontides, L.S.andKontos, V.S. (2004). Chronic Canine ehrlichiosis (Ehrlichiacanis): a retrospective study of 19 natural cases. J. Am. Anim. Hosp. Assoc., 40(3): 174-184
- Mylonakis, M.E., Siarkou, V. and Koutinas, A.F. (2010). Myelosuppressive canine monocyticehrlichiosis (Ehrlichiacanis): An update on the pathogenesis, diagnosis and management. Isr. J. Vet. Med., 65(4):129-134
- Mylonakis, M.E. and Theodorou, K.N. (2017). Canine monocyticehrlichiosis: an update on diagnosis and treatment. Acta Vet-Beograd, 67(3): 299-317
- Priyadarshini, M., Veena, J.D., Carunya, R., Mannan, H. L., Rastogi, D.P. (2019). Ovarian Sertoli-Leydig cell tumor with estrogenic manifestations in a postmenopausal lady: a case report. Indian J. Surg. Oncol., 10(3): 499–501
- Quartuccio, M., Marino, G., Garufi, G., Cristarella, S., Zanghì, A. (2012). Sertoli cell tumors associated with feminizing syndrome and spermatic cord torsion in two cryptorchid dogs. J. Vet. Sci., 13(2): 207–209
- Rabban, J.T., Soslow, R.A and Zaloudek, C.Z. (2010). Immunohistology of the female genital tract. In: Dabbs, D. J. (Ed.) Diagnostic immunohistochemistry, theranostic and genomic applications. Elsevier Inc., pp. 690-762
- Sainz, A., Roura, X., Miro, G., Estrada-Pena, A., Kohn, B., Harrus, S. and Solano-Gallego, L. (2015). Guidelines for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Para. &Vect., 8:75
- Schaefer, J.J., Needham, G.R., Bremer, W.G., Rikihisa, Y., Ewing, S.A., Stich, R.W. (2007). Tick acquisition of Ehrlichiacanis from dogs treated with doxycycline hyclate. Antimicrob. Agents &Chemother., 51: 3394-3396
- Schulz, B.S., Hupfauer, S., Ammer, H., Sauter-Louis, C., Hartmann, K. (2011). Suspected side effects of doxycycline use in dogs - a retrospective study of 386 cases. Vet. Rec., 169(9): 229
- Sivasudharsan, L., Devarathan, J., Mahesh, R., Kamalalar, G., Rambabu, K., Sumiran, N. and Sailaja, N. (2017). Sertoli cell tumor in a dog: a case report. Indian J. CaninePract., 9(1): 75–77
- Švara T, Gombač M, Pogorevc E, Plavec T, Zrimšek P, Pogaenik M. A. (2014). Retrospective study of canine testicular tumors in Slovenia. Slov. Vet. Res., 51:81–88
- Tsuzuki, Y., Kikuchi, I., Nojima, M., Yoshida, K., Hashizume, A., Tomita, S. (2017). A case report: ovarian Sertoli-Leydig cell tumor with hyperestrogenism and endometrial hyperplasia in a postmenopausal woman. Jpn. J.Clin. Med., 8: 1179066017695239
- Valente, P.C.L.G., Couto, R.M., Gamba, C.O., Vasconcelos, A.V., Leme, F.O.P, Ecco, R. and Paes, P.R.O. (2017). Bone marrow bi-hypoplasia in a dog with a sertoli cell tumor. Arq. Bras. Med. Vet. Zootec., 69(1): 95-100
- Waner, T. and Harrus, S. (2013). Canine monocyticehrlichiosis: from pathology to clinical manifestations. Isr. J. Vet. Med., 68:12-18
- Young,R.H. (2005). Sex cord-stromal tumors of the ovary and testis: their similarities and differences with consideration of selected problems. ModPathol., 18: S81-S98
- Zambarda, T.T., Renata, Q. S., Sonne, L., Pöppl, A.G. (2019). Müller’s duct persistence syndrome associated with cryptorchidism, sertoli cell tumor and pyometra in a miniature schnauzer – a case report. Acta Sci. Vet., 47(1): 446-451
References
Banco, B., Veronesi, M.C, Giudice, C., Rota, A., Grieco, V. (2012). Immunohistochemical evaluation of the expression of Anti-Müllerian Hormone in mature, immature and neoplastic Canine Sertoli cells. J. Comp. Path., 146(1): 18-23
Cahua, U.J., Sandoval, C.N., Fernández, A.V. Puicón, N.V. (2015). Pyometra and Sertoli cell tumor in a dog with persistent Müller duct. Rev. Invest. Vet. Perú (RIVEP), 26(4): 705-711
Carreira, V.S., Langohr, I.M., Ferrari, H.F., RuiLuvizotto, M.C. (2012). Pathology in practice: Sertoli cell tumor (SCT). J. Am. Vet. Med. Assoc., 241(1): 55-57
Castro, J.L.C., Albernaz, V.G.P., Domingues, C.M.T., Ferreira, A.A., Dittrich, G., Moratelli, C.B.S., Freitag, F.V. Miara, L.C. (2016). Malignant Sertoli cell tumor with abdominal infiltrative metastasis in a dog. Pak. Vet. J., 36(3): 379-381
Dhliwayo, S., Chihambakwe, B., Taonezvi, K., Chikerema, S.M., Tivapasi, M.T. and Pfukenyi, D.M. (2019). Seroprevalence of Canine Ehrlichiosis and Microscopic Screening for Canine Babesiosis in Dogs in Harare, Zimbabwe, 2016-2017. Vet. Med. Int., Article ID 4130210, https://doi.org/10.1155/2019/4130210
Ferguson, L. and Agoulnik, A.I. (2013). Testicular cancer and cryptorchidism. Front. Endocrinol., 4 (32): 1-9
Fourie, J.J., Horak, I., Crafford, D., Erasmus, H.L., Botha, O.J. (2015). The efficacy of a generic doxycycline tablet in the treatment of Canine monocyticEhrlichiosis. J. S. Afr. Vet. Assoc., 86:1193
Gaunt, S., Beall, M., Stillman, B., Lorentzen, L., Diniz, P., Chandrashekar, R. Breitschwerdt, E. (2010). Experimental infection and co-infection of dogs with Anaplasmaplatys and Ehrlichiacanis: hematologic, serologic and molecular findings. Para. &Vect., 3 (1): 33
Grieco, V., Riccardi, E., Greppi, G.F., Teruzzi, F., Iermano, V., Finazzi, M. (2008). Canine testicular tumours: a study on 232 dogs. J. Comp. Pathol., 138(2): 86–89
Harrus, S., Warner, T., Neer, M. (2012). Ehrlichiacanis infection. In: Sykes, J. and Greene, C., (Eds.) Infectious Diseases of the Dog and Cat. Elsevier Saunders, St. Louis, Missouri, pp. 227-238
Harrus, S. and Waner, T. (2011). Diagnosis of Canine monocytotropicEhrlichiosis (Ehrlichiacanis): an overview. Vet. J., 187: 292-296
Herndon, A.M., Casal, M.L., Jaques, J.T.S. (2012). Testicular neoplasia in the retained testicles of an intersex male dog. J. Am. Anim. Hosp. Assoc., 48(2): 118-124
Hohsteter, M., Artuskovic, M., Severin, K., Kurilj, A.G., Beck, A., Sostaric-Zuckermann, I.C., Grabarevic, Z. (2014). Canine testicular tumors: two types of seminomas can be differentiated by immunohistochemistry. Vet. Res., 10: 1-9
Holst, B.S. and Dreimanis, U. (2015). Anti-Müllerian hormone: a potentially useful biomarker for the diagnosis of canine Sertoli cell tumours. BMC Vet. Res., 11: 166-172
Hornakova, T., Vrbovska, M., Pavlak, A., Valencakova-Agyagosova, M., Halo, J. H. (2017). The evaluation of blood concentrations of testosterone, 17β-oestradiol and anti-Mullerian hormone in dogs with cryptorchidism and testicular tumours. Pol. J. Vet. Sci., 20(4): 677-685
Jain, N.C. (1986). Schalm’s Veterinary Haematology. 4th Edition, Lea and Febiger, Philadelphia, U.S.A., pp. 58-90
Kaewmongkol, G., Maneesaay, P., Suwanna, N., Tiraphut, B., Krajarngjang, T., Chouybumrung, A., Kaewmongkol, S., Sirinarumitr, T., Jittapalapong, S., Fenwick, S.G. (2016). First Detection of Ehrlichiacanis in cerebrospinal fluid from a non-thrombocytopenic dog with meningo-encephalitis by broad-range PCR. J. Vet. Intern. Med., 30(1): 255-259
Kang, S.C., Yang, H.S., Jung, J.Y., Jung, E.H., Lee, H.C. (2011). Malignant Sertoli cell tumor in Shih Tzu dog. Korean J. Vet. Res., 51:171-175
Kottadamane, M.R., Dhaliwal, P.S. and Singla, L.D. (2016). Diagnosis and treatment of canine monocyticehrlichiosis in a boxer breed of dog – a case report. Intern. J. Sci. Envt. Tech., 5(5): 3099 – 3105
Kukreti, K., Das, M.K., Rastogi, A.K., Dubey, R., Pandey, L.K. and Sharma, P. (2018). Prevalence of Canine MonocyticEhrlichiosis in Canine Population Across India. Arch. Razi Inst., 73(2): 87-93
Lawrence, J.A. and Saba, C.F. (2013). Tumors of the male Reproductive system. In: Withrow and MacEwen (Eds.) Small Animal Clinical Oncology. Elsevier Saunders, USA, pp. 557–571
Liao, A.T., Chu, P.Y., Yeh, L.S., Lin, C.T., Liu, C.H. (2009). A 12-year retrospective study of canine testicular tumors. J. Vet. Med. Sci., 71(7): 919–923
Mohamed, S., Bauchet, A-L., Fiette, L. (2017). Tissue sampling and processing for histopathology evaluation. Histopathology procedures. In: Gautier, J-C. (Ed.) Drug Safety Evaluation: methods and protocols, methods in molecular biology. Springer Science+Business Media LLC, pp. 101-114
Mylonakis, M.E., Koutinas, A.F., Breitscwerdt, E.B., Hegarty, B.C., Billinis, C.D., Leontides, L.S.andKontos, V.S. (2004). Chronic Canine ehrlichiosis (Ehrlichiacanis): a retrospective study of 19 natural cases. J. Am. Anim. Hosp. Assoc., 40(3): 174-184
Mylonakis, M.E., Siarkou, V. and Koutinas, A.F. (2010). Myelosuppressive canine monocyticehrlichiosis (Ehrlichiacanis): An update on the pathogenesis, diagnosis and management. Isr. J. Vet. Med., 65(4):129-134
Mylonakis, M.E. and Theodorou, K.N. (2017). Canine monocyticehrlichiosis: an update on diagnosis and treatment. Acta Vet-Beograd, 67(3): 299-317
Priyadarshini, M., Veena, J.D., Carunya, R., Mannan, H. L., Rastogi, D.P. (2019). Ovarian Sertoli-Leydig cell tumor with estrogenic manifestations in a postmenopausal lady: a case report. Indian J. Surg. Oncol., 10(3): 499–501
Quartuccio, M., Marino, G., Garufi, G., Cristarella, S., Zanghì, A. (2012). Sertoli cell tumors associated with feminizing syndrome and spermatic cord torsion in two cryptorchid dogs. J. Vet. Sci., 13(2): 207–209
Rabban, J.T., Soslow, R.A and Zaloudek, C.Z. (2010). Immunohistology of the female genital tract. In: Dabbs, D. J. (Ed.) Diagnostic immunohistochemistry, theranostic and genomic applications. Elsevier Inc., pp. 690-762
Sainz, A., Roura, X., Miro, G., Estrada-Pena, A., Kohn, B., Harrus, S. and Solano-Gallego, L. (2015). Guidelines for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Para. &Vect., 8:75
Schaefer, J.J., Needham, G.R., Bremer, W.G., Rikihisa, Y., Ewing, S.A., Stich, R.W. (2007). Tick acquisition of Ehrlichiacanis from dogs treated with doxycycline hyclate. Antimicrob. Agents &Chemother., 51: 3394-3396
Schulz, B.S., Hupfauer, S., Ammer, H., Sauter-Louis, C., Hartmann, K. (2011). Suspected side effects of doxycycline use in dogs - a retrospective study of 386 cases. Vet. Rec., 169(9): 229
Sivasudharsan, L., Devarathan, J., Mahesh, R., Kamalalar, G., Rambabu, K., Sumiran, N. and Sailaja, N. (2017). Sertoli cell tumor in a dog: a case report. Indian J. CaninePract., 9(1): 75–77
Švara T, Gombač M, Pogorevc E, Plavec T, Zrimšek P, Pogaenik M. A. (2014). Retrospective study of canine testicular tumors in Slovenia. Slov. Vet. Res., 51:81–88
Tsuzuki, Y., Kikuchi, I., Nojima, M., Yoshida, K., Hashizume, A., Tomita, S. (2017). A case report: ovarian Sertoli-Leydig cell tumor with hyperestrogenism and endometrial hyperplasia in a postmenopausal woman. Jpn. J.Clin. Med., 8: 1179066017695239
Valente, P.C.L.G., Couto, R.M., Gamba, C.O., Vasconcelos, A.V., Leme, F.O.P, Ecco, R. and Paes, P.R.O. (2017). Bone marrow bi-hypoplasia in a dog with a sertoli cell tumor. Arq. Bras. Med. Vet. Zootec., 69(1): 95-100
Waner, T. and Harrus, S. (2013). Canine monocyticehrlichiosis: from pathology to clinical manifestations. Isr. J. Vet. Med., 68:12-18
Young,R.H. (2005). Sex cord-stromal tumors of the ovary and testis: their similarities and differences with consideration of selected problems. ModPathol., 18: S81-S98
Zambarda, T.T., Renata, Q. S., Sonne, L., Pöppl, A.G. (2019). Müller’s duct persistence syndrome associated with cryptorchidism, sertoli cell tumor and pyometra in a miniature schnauzer – a case report. Acta Sci. Vet., 47(1): 446-451