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Submitted
Dec 4, 2020
Accepted
May 15, 2021
Published
Jun 30, 2021
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Abstract
In a study to compare the durability of commonly used stains (Giemsa, Leishman, Wright, Eosin, Nigrosin and Gentian violet) for exfoliative vaginal cytology, vaginal smear was obtained from eleven apparently healthy West African Dwarf (WAD) female Goats and processed according to standard technique. Scores (0-3) were given on four parameters namely background of smears, overall staining pattern, cytoplasmic staining and nuclear staining. Quality index one (QI-I) was calculated from the ratio of score achieved to the maximum score possible (12), immediately post staining while quality index–II (QI-II) was obtained 35 days after. Calculation for durability index (DI) was self-derived and equalled to ratio of QI-II to QI-I. The data were presented as mean ± SEM. Multinomial logistic regression model was generated for the QI-I and QI-II using durability index as reference category. Giemsa, Leishman and Wright stains were more durable than others with their mean DI values significantly (P < 0.05) higher than Gentian violet, Nigrosin and Eosin.The model showed 89.2% overall model accuracy for the multinomial logistic regression model and 81.5% for the multinomial Bayes Naïve regression model. In conclusion, Giemsa, Leishman and Wright stains were more reliable and durable for exfoliative vaginal cytology compared to the other stains.
Keywords
Durability; Oestrus; Stains; Vaginal cytology
Article Details
How to Cite
Ani, N. V., Ogundunmade, T. P., Daniel, D., Raheem, K. A., Odirichukwu, E. O., & Osuagwuh, U. I. (2021). Comparative Durability of Common Stains Used for Exfoliative Vaginal Cytology: Stains for vaginal cytology. Sahel Journal of Veterinary Sciences, 18(2), 9-16. https://doi.org/10.54058/saheljvs.v18i2.220
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