Article Sidebar

Submitted
Nov 4, 2020
Accepted
Mar 30, 2021
Published
Apr 5, 2021
Download
PDF
Statistic
Read Counter : 490 Download : 188

Downloads

Download data is not yet available.

Main Article Content

Abstract

We evaluated the efficacy of castration with bilateral intratesticular injections of Calcium Chloride Dihydrate (CaCl2) in ethanol, Olive Oil as well as Burdizzo technique in twenty Sahel bucks. The bucks were randomly distributed into four groups as A, B, C and D. Group A served as the control and were given bilateral intratesticular injection of 1.0 ml of normal saline. Bucks in group B were castrated with Burdizzo, while group C and D were given bilateral intra testicular injection of 1.0 ml of CaCl2 and Olive oil, respectively. The sonograms and semen profiles of the bucks were evaluated.The diameters of the testicles and spermatic cords and the semen parameters were measured in real time after castration at days 1, 7, 14, 21, 28 and 60. The diameters in the Burdizzo, CaCl2‎and Olive oil castrated bucks ‎significantly increased (P < 0.05) on days 7 and was decreased at ‎day 60.‎ Semen motility was absent in the bucks castrated with Cacl2 after day 14. The ‎concentration of spermatozoa also decreased significantly on day 7 in all the Burdizzo, CaCl2‎and ‎Olive oil castrated goats. Sonogram of testes castrated with Cacl2 showed discreet focal ‎hyperechoic, surrounded by ‎hypoechoic areas ‎within the parenchyma due to the CaCl2 deposition. The testes of bucks castrated with olive oil were ‎characterized by a central hypoechoic area ‎surrounded by clearly distinguishable hyperechoic ‎areas within the parenchyma. It was concluded that intratesticular injection with Burdizzo, CaCl2and olive oil resulted in successful castration but CaCl2 injection resulted in earlier azoospermia.

Keywords

Burdizzo; Castration; Calcium chloride; Goats; Olive oil; Sahel bucks

Article Details

How to Cite
Laku, D., Mohammed, A., & Bukar, M. M. (2021). Effectiveness of Castration with Burdizzo, Calcium Chloride and Olive Oil in ‎Sahel Bucks. Sahel Journal of Veterinary Sciences, 18(1), 13-20. https://doi.org/10.54058/saheljvs.v18i1.213

References

  1. Al-Asadi R and Al-Kadi KK (2012). Non-surgical castration ‎in bucks a comparative study between chemical ‎castration and external ligation of the spermatic ‎cord. AL-Qadisiyah Journal of Veterinary Medicine ‎Sciences,11 (1):92-102‎
  2. Andrade A, Soares A, Freitas F, Silva S, Peña-Alfaro C, ‎Batista A and Guerra M (2014). Testicular and ‎epididymal ultrasonography in Santa Inês lambs ‎raised in Brazil. Animal Reproduction, 11:110-118‎
  3. Bakir B, Gulyuz F, Karaca F, Yuksel H, Sahin A and Uslu BA ‎‎(2002). Chemical castration in dogs. Journal of ‎Health Science,8 (1-3):6-8‎
  4. Cavalieri J (2017). Chemical sterilisation of animals: A ‎review of the use of zinc-and CaCl2 based solutions ‎in male and female animals and factors likely to ‎improve responses to treatment. Animal ‎Reproduction Science, 181:1-8‎
  5. Currah JM, Hendrick SH and Stooley JM (2009). The ‎behavioral assesment and alleviation of pain ‎associated with castration in beef calves treated ‎with flunixin meglumine and cauda lidocaine ‎epidural anesthesia with epinephrine. Canadian ‎Veterinary Journal, 50:375-382‎
  6. Da RP, Mustafa AS and Talwar GP (1982). Atrophy of ‎seminiferous tubules in mouse testes after ‎intratesticular injection of BCG and their ‎regeneration Archive of Andrology, 9 (3):244-250‎
  7. De Faveri D, Aliakbarian B, Avogardo M, Perego P and ‎Converti A (2008). Improvement of olive oil ‎phenolic content by means of enzyme formulation: ‎Effects of different enzyme activities and levels. ‎Biomedical Engineering Journal, 41:149-156‎
  8. Fordyce D, Hodge PB, Beaman NJ, Laing AR, Campero C ‎and Shepherd RK (1989). An evaluation of calf ‎castration by intratesticular injection of lactic acid ‎solution Australian Veterinary journal, 66:272-276‎
  9. Gouletsou PG, Amiridis GS, Cripps PJ, Lainas T, Deligianis K, ‎Saratsis P and Fthenakis GC (2003). ‎Ultrasonographic appearance of clinically healthy ‎testicles and epididymis of rams. Theriogenology, ‎‎59:1959-1972‎
  10. Hasan M, Miah M, Rosy T, Jha P and Juyena N (2016). ‎Serum testosterone concentration in surgically ‎castrated Black Bengal goats. Bangladesh ‎Veterinarian, 33 (2):71-77‎
  11. Ibrahim A, Ali MM, Abou-Khalil NS and Ali MF (2016). ‎Evaluation of chemical castration versus surgical ‎castration in donkeys: testosterone as an endpoint ‎marker. BMC Veterinary Research, 12:46‎
  12. Ijaz A, Abalkhail AA and Khamas WAH (2000). Effect of ‎intratesticular injection of formalin on seminiferous ‎tubules in Awasi lambs. Pakistan Veterinary ‎Journal,20 (3):129-134‎
  13. Jana K and Samanta PK (2007). Sterilization of male dogs ‎with a single intratesticular injection of calcium ‎chloride a dose dependent study Contraception, ‎‎75:390-400‎
  14. Jana K, Samanta PK and Gosh D (2005). Evaluation of ‎single intratesticular injection of calcium chloride ‎for nonsurgical sterilization of male Black Bengal ‎goats Capra hircus: a dose-dependent study. ‎Animal Reproduction Science, 86 89-108‎
  15. Leoci R, Aiudi G, Cicirelli V, Brent L, Iaria C and Lacalandra ‎GM (2019). Effects of intratesticular vs ‎intraepididymal calcium chloride sterilant on ‎testicular morphology and fertility in dogs. ‎Theriogenology, 4756-61‎
  16. Leoci R, Aiudi G, Slvestre F, Lissner E and Lacalandra G ‎‎(2014). A dose-finding, long-term study on the use ‎of calcium chloride in saline solution as of non-‎surgical sterilization in dogs Acta Veterinaria ‎Scandanavia, 56:63 ‎
  17. Mohammed A and James F (2013). Chemical castration by ‎a single bilateral intra-testicular injection of ‎chlorhexidine gluconate and cetrimide in bucks. ‎Sokoto Journal of Veterinary Sciences11 (1):62-65‎
  18. Molony V, Kent JE and Robertson IS (1995). Assessment of ‎acute and chronic pain after different methods of ‎castration of calves. Application of Animal ‎Behavioural Science, 46:33-48‎
  19. Neto OA, Gasperin BG, Rovani MT, Ilha GF, Nóbrega Jr JE, ‎Mondadori RG, Gonçalves PB and Antoniazzi AQ ‎‎(2014). Intratesticular hypertonic sodium chloride ‎solution treatment as a method of chemical ‎castration in cattle. Theriogenology, 82 (7):1007-‎‎1011‎
  20. Olatunji-Akioye A, Afonja V and Saka B (2018). ‎Ultrasonographic Features of Testes of West ‎African Dwarf Bucks. Iranian Journal of ‎Veterinary Surgery, 13 (2):34-39‎
  21. Paiva MF, Rodrigues V, Calheirs R and Marques MPJ ‎‎(2011). Characterization of antioxidant olive oil ‎biophenols by spectroscopic methods. Science Food ‎and Agriculture. (2):309-314.‎
  22. Rajkumar D (2013). Minimization of pain in cattle ‎castration with respect to method, age and pain ‎relief. In, vol. http://vip.vetsci.usyd.edu.au/content ‎upload/content-‎‎3164/RajkumarDharshinea.pdf/retrieved, ‎
  23. Sakthivel J, De A, Kundu A, Roy K, Sunder J, Kundu M, ‎Balakrishnan M, Chand S and Ahmed Z (2013). ‎Sonographic characteristics of goat testis on water ‎bath based ultrasonography, vol 152. pp 79-87‎
  24. Sawhney P (2016). Burdizzo versus pinhole castration in ‎bucks and cattle calves. In, vol. Nanaji Deshmukh ‎Veterinary Science University Jabalpur, ‎
  25. Sen C, Yumusak N, Faundez R, Temamogullari F and ‎Taskin A (2017). Evaluation of intra-testicular ‎injections of calcium chloride and 4-‎vinylcyclohexene 1, 2 monoepoxide for chemical ‎sterilization in guinea pigs. Polish Journal of ‎Veterinary Sciences, 20 (2):251-260‎
  26. Stafford KJ, Mellor DJ and McMeekan CM (2000). A survey ‎of the methods used by farmers to castrate calves in ‎New Zealand. New Zealand Veterinary Journal, ‎‎48:16-18‎
  27. Stoffel MH, VonRotz A, Kocher M, Merkli M, Boesch D. and ‎Steiner A (2009). Histological assessment of ‎testicular residues in lambs and calves after ‎Burdizzo Castration. Veterinary Research, 164:523-‎‎527‎
  28. Visioli F, Poli A and Galli C (2002). Antioxidant and other ‎biological activities of phenols from olives and olive ‎oil. Journal of Medicinal Research Review, 22:65-‎‎75‎
  29. Webe JP and Barr KJ (1984.). The control of male fertility by ‎‎1,2,3-trihydroxypropan [THP;glycerol]; rapid arrest ‎of spermatogenesis without altering libido, ‎accessory organs, gonadal steroidogenesis and ‎serum testosterone, LH and FSH. Journal of ‎Contraception,29.:291-30‎