Article Sidebar
Main Article Content
Abstract
Wound healing is of paramount importance in Veterinary Surgery whenever skin integrity is breached. The faster the healing rate, the better chances to mitigate contamination and infections. There is paucity of information on the use of hyaluronic acid in wound healing in Veterinary medicine. Twenty (20) clinically healthy Wister rats of both sexes were randomly grouped in to four groups (A, B, C, D) of five rats each and allowed to acclimatize for two weeks. Anesthesia was carried out using a combination of xylazine and ketamine at a dosage rate of 5mg/kg and 50mg/kg respectively, intraperitoneal. Circular skin excision was made on each rat after shaving and scrubbing using 70% ethyl alcohol. Group A rats served as negative control while group B and C served as positive controls povidone iodine and oxytetracycline spray were applied topically respectively, group D served as test group where hyaluronic acid serum was applied topically, healing was monitored for 18 days. Results (macroscopy and histology) shows group D having significant healing rate (p<0.05) compared to A, B and C. Hyaluronic acid serum used in this study was seen to have a significant wound healing contraction potential compared to povidone iodine, oxytetracycline spray and the negative control.
Keywords
Article Details
How to Cite
References
- Alven, S., and Aderibigbe, B. A. (2021). Hyaluronic Acid-Based Scaffolds as Potential Bioactive Wound Dressings. Polymers, 13(13), 2102. https://doi.org/10.3390/polym13132102
- Boeriu, C. G., Springer, J., Kooy, F. K., van den Broek, L. A. M., and Eggink, G. (2013). Production methods for hyaluronan. International Journal of Carbohydrate Chemistry, 2013, 14.doi: 10.1155/2013/624967.
- Caetano, G. F., Frade, M. A., Andrade, T. A., Leite, M. N., Bueno, C. Z., Moraes, Â. M., and Ribeiro-Paes, J. T. (2015). Chitosan-alginate membranes accelerate wound healing. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 103(5), 1013–1022. https://doi.org/10.1002/jbm.b.33277
- Fallacara, A., Baldini, E., Manfredini, S., and Vertuani, S. (2018). Hyaluronic acid in the third millennium. Polymers, 10, 701.
- Fries, R. B., Wallace, W. A., and Roy, S. (2005). Dermal excisional wound healing in pigs following treatment with topically applied pure oxygen. Mutation Research, 579:172-181.
- Jamadagni, P. S., Jamadagni, S., Mukherjee, K., Upadhyay, S., Gaidhani, S. and Hazra, J. (2016). Experimental and histopathological observation scoring methods for evaluation of wound healing properties of JatyadiGhrita. Ayu, 37(3-4), 222–229. https://doi.org/10.4103/ayu.AYU_51_17
- Kawano, Y., Patrulea, V., Sublet, E., Borchard, G., Iyoda, T., Kageyama, R., Morita, A., Seino, S., Yoshida, H., and Jordan, O. (2021). Wound healing promotion by hyaluronic acid: Effect of molecular weight on gene expression and in vivo wound closure. Pharmaceuticals, 14, 301.https://doi.org/10.3390/ph14040301
- Junker, J. P., Kamel, R. A., Caterson, E. J., and Eriksson, E. (2013). Clinical impact upon wound healing and inflammation in moist, wet, and dry environments. Advances in Wound Care, 2(7), 348-356.doi: 10.1089/wound.2012.0412. PMID: 24587972; PMCID: PMC3842869.
- Koschwanez, H. E., and Broadbent, E. (2011). The use of wound healing assessment methods in psychological studies: a review and recommendations. British Journal of Health Psychology, 16, 1-32.
- Leite, S. N., Andrade, T. A., Masson-Meyers, D. S., Leite, M. N., Enwemeka, C. S., and Frade, M. A. (2014). Phototherapy promotes healing of cutaneous wounds in undernourished rats. AnaisBrasileiros de Dermatologia, 89(6), 899-904.
- Leite, M. N. and Frade, M. A. C. (2021). Efficacy of 0.2% hyaluronic acid in the healing of skin abrasions in rats. Heliyon, 7(7), e07572. https://doi.org/10.1016/j.heliyon.2021.e07572
- Li, L., Heldin, C. H. and Heldin, P. (2006). Inhibition of platelet-derived growth factor-BB-induced receptor activation and fibroblast migration by hyaluronan activation of CD44. The Journal of biological chemistry, 281(36), 26512–26519. https://doi.org/10.1074/jbc.M605607200
- Litwiniuk, M., Krejner, A., Speyrer, M. S., Gauto, A. R. and Grzela, T. (2016). Hyaluronic Acid in Inflammation and Tissue Regeneration. Wounds : a compendium of clinical research and practice, 28(3), 78–88.
- Masson-Meyers, D. S., Andrade, T. A., Leite, S. N., and Frade, M. A. (2013). Cytotoxicity and wound healing properties of Copaiferalangsdorffii oleoresin in rabbits. International Journal of Natural Product Science, 3, 10-20.
- Mulkalwar, S., Behera, L., Golande, P., Manjare, R., and Patil, H. (2015). Evaluation of wound healing activity of topical phenytoin in an excision wound model in rats. International Journal of Basic & Clinical Pharmacology, 4, 139-143.
- Nauta, A.C., Gurtner, G.C., and Longaker, M.T. (2013). Adult stem cells in small animal wound healing models. In G.R. Gourdie and T.A. Myers (Eds.), Wound Regeneration and Repair: Methods and Protocols. Humana Press. pp. 81-98. New York, NY.
- Necas, J.,Bartosicova, L., Brauner, P., and Kolar, J. (2008). Hyaluronic acid (hyaluronan): a review. Veterinary Medicine, 8, 397-411.
- Nyman, E., Henricson, J., Ghafouri, B., Anderson, C. D., and Kratz, G. (2019). Hyaluronic acid accelerates re-epithelialization and alters protein expression in a human wound model. Plastic and Reconstructive Surgery; Global Open, 7(5), e2221.
- Olczyk, P., Mencner, Ł., and Komosinska-Vassev, K. (2014). The role of the extracellular matrix components in cutaneous wound healing. Biomedical Research International, 2014, 12-24.
- Papakonstantinou, E., Roth, M. and Karakiulakis, G. (2012). Hyaluronic acid: A key molecule in skin aging. Dermato-endocrinology, 4(3), 253–258. https://doi.org/10.4161/derm.21923
- Stephens, P., Caley, M., and Peake, M. (2013). Alternatives for animal wound model systems. In G.R. Gourdie and T.A. Myers (Eds.), Wound Regeneration and Repair: Methods and Protocols. Humana Press. pp. 177-201. New York, NY.
- Toole B. P. (2004). Hyaluronan: from extracellular glue to pericellular cue. Nature reviews. Cancer, 4(7), 528–539. https://doi.org/10.1038/nrc1391
- Vigetti, D., Karousou, E., Viola, M., Deleonibus, S., De Luca, G., and Passi, A. (2014). Hyaluronan: biosynthesis and signaling. BiochimicaetBiophysica Acta - General Subjects, 1840(8), 2452-2459.
- Wong, V.W., Sorkin, M., Glotzbach, J.P., Longaker, M.T., and Gurtner, G.C. (2011). Surgical approaches to create murine models of human wound healing. Journal of Biomedical Biotechnology, 2011, 969618.
References
Alven, S., and Aderibigbe, B. A. (2021). Hyaluronic Acid-Based Scaffolds as Potential Bioactive Wound Dressings. Polymers, 13(13), 2102. https://doi.org/10.3390/polym13132102
Boeriu, C. G., Springer, J., Kooy, F. K., van den Broek, L. A. M., and Eggink, G. (2013). Production methods for hyaluronan. International Journal of Carbohydrate Chemistry, 2013, 14.doi: 10.1155/2013/624967.
Caetano, G. F., Frade, M. A., Andrade, T. A., Leite, M. N., Bueno, C. Z., Moraes, Â. M., and Ribeiro-Paes, J. T. (2015). Chitosan-alginate membranes accelerate wound healing. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 103(5), 1013–1022. https://doi.org/10.1002/jbm.b.33277
Fallacara, A., Baldini, E., Manfredini, S., and Vertuani, S. (2018). Hyaluronic acid in the third millennium. Polymers, 10, 701.
Fries, R. B., Wallace, W. A., and Roy, S. (2005). Dermal excisional wound healing in pigs following treatment with topically applied pure oxygen. Mutation Research, 579:172-181.
Jamadagni, P. S., Jamadagni, S., Mukherjee, K., Upadhyay, S., Gaidhani, S. and Hazra, J. (2016). Experimental and histopathological observation scoring methods for evaluation of wound healing properties of JatyadiGhrita. Ayu, 37(3-4), 222–229. https://doi.org/10.4103/ayu.AYU_51_17
Kawano, Y., Patrulea, V., Sublet, E., Borchard, G., Iyoda, T., Kageyama, R., Morita, A., Seino, S., Yoshida, H., and Jordan, O. (2021). Wound healing promotion by hyaluronic acid: Effect of molecular weight on gene expression and in vivo wound closure. Pharmaceuticals, 14, 301.https://doi.org/10.3390/ph14040301
Junker, J. P., Kamel, R. A., Caterson, E. J., and Eriksson, E. (2013). Clinical impact upon wound healing and inflammation in moist, wet, and dry environments. Advances in Wound Care, 2(7), 348-356.doi: 10.1089/wound.2012.0412. PMID: 24587972; PMCID: PMC3842869.
Koschwanez, H. E., and Broadbent, E. (2011). The use of wound healing assessment methods in psychological studies: a review and recommendations. British Journal of Health Psychology, 16, 1-32.
Leite, S. N., Andrade, T. A., Masson-Meyers, D. S., Leite, M. N., Enwemeka, C. S., and Frade, M. A. (2014). Phototherapy promotes healing of cutaneous wounds in undernourished rats. AnaisBrasileiros de Dermatologia, 89(6), 899-904.
Leite, M. N. and Frade, M. A. C. (2021). Efficacy of 0.2% hyaluronic acid in the healing of skin abrasions in rats. Heliyon, 7(7), e07572. https://doi.org/10.1016/j.heliyon.2021.e07572
Li, L., Heldin, C. H. and Heldin, P. (2006). Inhibition of platelet-derived growth factor-BB-induced receptor activation and fibroblast migration by hyaluronan activation of CD44. The Journal of biological chemistry, 281(36), 26512–26519. https://doi.org/10.1074/jbc.M605607200
Litwiniuk, M., Krejner, A., Speyrer, M. S., Gauto, A. R. and Grzela, T. (2016). Hyaluronic Acid in Inflammation and Tissue Regeneration. Wounds : a compendium of clinical research and practice, 28(3), 78–88.
Masson-Meyers, D. S., Andrade, T. A., Leite, S. N., and Frade, M. A. (2013). Cytotoxicity and wound healing properties of Copaiferalangsdorffii oleoresin in rabbits. International Journal of Natural Product Science, 3, 10-20.
Mulkalwar, S., Behera, L., Golande, P., Manjare, R., and Patil, H. (2015). Evaluation of wound healing activity of topical phenytoin in an excision wound model in rats. International Journal of Basic & Clinical Pharmacology, 4, 139-143.
Nauta, A.C., Gurtner, G.C., and Longaker, M.T. (2013). Adult stem cells in small animal wound healing models. In G.R. Gourdie and T.A. Myers (Eds.), Wound Regeneration and Repair: Methods and Protocols. Humana Press. pp. 81-98. New York, NY.
Necas, J.,Bartosicova, L., Brauner, P., and Kolar, J. (2008). Hyaluronic acid (hyaluronan): a review. Veterinary Medicine, 8, 397-411.
Nyman, E., Henricson, J., Ghafouri, B., Anderson, C. D., and Kratz, G. (2019). Hyaluronic acid accelerates re-epithelialization and alters protein expression in a human wound model. Plastic and Reconstructive Surgery; Global Open, 7(5), e2221.
Olczyk, P., Mencner, Ł., and Komosinska-Vassev, K. (2014). The role of the extracellular matrix components in cutaneous wound healing. Biomedical Research International, 2014, 12-24.
Papakonstantinou, E., Roth, M. and Karakiulakis, G. (2012). Hyaluronic acid: A key molecule in skin aging. Dermato-endocrinology, 4(3), 253–258. https://doi.org/10.4161/derm.21923
Stephens, P., Caley, M., and Peake, M. (2013). Alternatives for animal wound model systems. In G.R. Gourdie and T.A. Myers (Eds.), Wound Regeneration and Repair: Methods and Protocols. Humana Press. pp. 177-201. New York, NY.
Toole B. P. (2004). Hyaluronan: from extracellular glue to pericellular cue. Nature reviews. Cancer, 4(7), 528–539. https://doi.org/10.1038/nrc1391
Vigetti, D., Karousou, E., Viola, M., Deleonibus, S., De Luca, G., and Passi, A. (2014). Hyaluronan: biosynthesis and signaling. BiochimicaetBiophysica Acta - General Subjects, 1840(8), 2452-2459.
Wong, V.W., Sorkin, M., Glotzbach, J.P., Longaker, M.T., and Gurtner, G.C. (2011). Surgical approaches to create murine models of human wound healing. Journal of Biomedical Biotechnology, 2011, 969618.