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Abstract
Oxytetracycline is one of the commonly used antibiotics in veterinary practice in Nigeria. Questionnaire survey was conducted to ascertain the brands of oxytetracycline commonly used among veterinary clinicians, veterinary pharmacies, and livestock farmers. Spectrophotometric method was used to determine the actual concentration of some oxytetracycline brands sold over the counter for veterinary use. Five brands of oxytetracycline soluble powder were obtained from veterinary pharmacies in Maiduguri Metropolis. The percentage content of the oxytetracycline brands were 51.72%, 80.82%, 112.30%, 60.34% and 29.50% respectively. Four out of the five brands (80%) contained less than the labelled concentration. There was moderate positive correlation (r = 0.506) between the analyzed brands and their expiring dates. This implies that there is an ongoing administration of sub-standard antibiotics to animals with consequences including but not limited to development of resistance, therapy failure, toxicity, and side effects. Results of survey revealed 97.6% of the respondents use oxytetracycline and 41 different brands were available in Maiduguri. All (100%) the respondents either found oxytetracycline to be very effective or effective, while 80% of the respondents did not notice any side effects following use of the drug. It was concluded that oxytetracycline was widely used and regarded as an effective drug by the respondents but 80% of the brands had oxytetracycline concentration below the labelled concentration. Proper monitoring and quality control assurance should be enforced by regulatory agencies.
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References
- Abubakar, A. (2017). "Origin and meaning of Maiduguri." Retrieved from www.researchgate.net/publication/318215165_Origin_and_Meaning_of_Maiduguri.
- Aktas, I. and Yarsan, E. (2017). Pharmacokinetics of Conventional and Long-Acting Oxytetracycline Preparations in Kilis Goat. Front. Vet. Sci. 4, 229.
- Aliyu, E.H., Nma, B.A., Suleiman, Y. and Muhammed, K.Y. (2018) A survey of antimicrobial usage by animal health practitioners in Niger State. In: A Paper Presentation at the 55th Annual Congress of the Nigerian Veterinary Medical Association at Usmanu Danfodio University Sokoto, 22nd -27th, October, 2018
- Audu, S.A, Ahmed, A, Taiwo, A.E, SanI, M, Ojuolape, A.R., Sani, A.S., Bukola, A.R. and Mohammed, I. (2012). Comparative Quantitative Analysis of Different Brands of 300mg Aspirin Tablet Marketed in Maiduguri Metropolitan Council (MMC). IJPSR 3(8): 426-410
- Boxall, A.B.A, Fogg, L.A., Blackwell, P.A., Kay, P., Pemberton, E.J. and Croxford, A. (2004). Veterinary medicines in the environment. Rev. Environ. Contam. Toxicol., 180. 1-91
- Brunton, S., Carmichael, B., Fitzgerald, M., Liu, H., Varon, J. and Weiland, D. (2005). Community-acquired bacterial respiratory tract infections: consensus recommendations. J. Fam. Pract., 54(3): 255-262, PMID: 15755379.
- Cantrell, L., Suchard, J. R., Wu, A. and Gerona, R.R. (2012). Stability of active ingredients in long-expired prescription medications. Arch. Intern. Med., 172(21):1685–1687. https://doi.org/10.1001/archinternmed.2012.4501
- Daniel, N., Malgwi, K.D., Umaru, B., Omeh, I.J. and Sanya, L. (2021). A survey of the usage (frequency and pattern) of antibiotics at the University of Maiduguri Veterinary Teaching Hospital,Maiduguri, Borno State, Nigeria. Vet. World, 14(11): 2941-2946. www.doi.org/10.14202/vetworld.2021.2941-2946
- Dina, O.A. and Arowolo R.O.A. (1991): Some considerations on veterinary drug use and supply in Nigeria. Re. d’Elevage Med. Vet. Pays Tropicaux, 44, 29-31.
- Erb, A., Sturmer, T., Marre, R. and Brenner, H. (2007). Prevalence of antibiotic resistance in Escherichia coli: overview of geographical, temporal, and methodological variations. Eur J Clin Microbiol Infect Dis, 26:8390. https://doi.org/10.1007/s10096-006-0248-2
- Ezenduka, E.V., Oboegbulem, S.I., Nwanta, J.A. and Onunkwo, J.I. (2011). Prevalence of antimicrobial residues in raw table eggs from farms and retail outlets in Enugu State, Nigeria. Trop. Anim. Health Prod., 43 (3): 557-559. https://doi.org/10.1007/s11250-010-9730-z
- Geidam, Y.A., Ibrahim, U.I., Grema, H.A., Sanda, K.A., Suleiman, A. and Mohzo, D.L. (2012) Patterns of antibiotic sales by drug stores and usage in poultry farms: A questionnaire-based survey in Maiduguri, Northeastern Nigeria. J. Anim. Vet. Adv., 11(16): 2852-2855.
- Idowu, F., Junaid, K., Paul, A., Gabriel, O., Paul, A., Sati, N., Maryam, M. and Jarlath, U., (2010). Antimicrobial screening of commercial eggs and determination of tetracycline residue using two microbiological methods. Int. J. Poultry Sci., 9 (10): 959-962.
- Islam, M.S., Rahman, M.M., Bhuyian, M.M.U., Shamsuddin, M. and Islam, M.T. 2016. Efficacy of oxytetracycline, amoxicillin, sulfamethoxazole and trimethoprim, and tylosin for the treatment of bacterial diseases in cattle and goats. Bangladesh J. Vet. Med. 14(1): 47-51.
- Kaya, S. (2007). Kemoterapötikler. 2nd ed. In: Kaya S, Pirinçci İ, Bilgili A, editors. VeterinerFarmakoloji. (Vol. 2), Ankara: Medisan Press. pp. 327–488.
- Keita, A., Pommier, P., Pagot, E., Couper, A. and Cromie, L. (2007). A combination oxytetracycline/flunixin treatment of respiratory infections in cattle. Rev. Méd. Vét., 158: 86-91.
- Kovacs, S., Hawes, S.E., Maley, S.N., Mosites, E., Wong, L. and Stergachis, A. (2014) Technologies for detecting falsified and substandard drugs in low and middle-income countries. PLoS One, 9(3):e90601. https://doi.org/10.1371/journal.pone.0090601
- Levy, S. B. (1992). Active efflux mechanisms for antimicrobial resistance. Antimicrob. Agents Chemother., 36: 695–703. https://doi.org/10.1128/aac.36.4.695
- Levy, S.B. and Marshall, B. (2004). Antibacterial resistance worldwide: causes, challenges and responses. Nat Med, 10(12 Suppl): S122-129. https://doi.org/10.1038/nm1145
- Merey, H. A., Abd-Elmonem, M. S., Nazlawy, H. N. and Zaazaa, H. E. (2017). Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. J. Anal. Methods Chem.: 2321572. https://doi.org/10.1155/2017/2321572
- Moken, MC. (2003). Fake pharmaceuticals: how they and relevant legislation or lack thereof contribute to consistently high and increasing drug prices. Am. J Law Med, 29: 525–542. PMID: 15119247
- Nazerali, H. and Hogerzeil, H.V. (1998). The quality and stability of essential drugs in rural Zimbabwe: controlled longitudinal study. BMJ, 17(7157):512-513. https://doi.org/10.1136/bmj.317.7157.512
- Ojo, O.E., Oyekunle, M.A., Ogunleye, A.O. and Otesile, E.B. (2009). Escherichia coli, O157:H7 in Food animals in part of south-western Nigeria: Prevalence and in vitro antimicrobial susceptibility. Trop. Vet. 26 (3and4): 23-30
- Okeke, I.N., and Laxminarayan, R., Bhutta, Z.A., Duse, A.G., Jenkins, P., O'Brien, T.F., Pablos-Mendez, A. and Klugman, K.P. (2005). Antimicrobial resistance in developing countries. Part I: recent trends and current status. Lancet Infect. Dis.,5(8), 481–493. https://doi.org/10.1016/s1473-3099(05)70189-4
- Okeke, I.N., Aboderin, O.A., Byarugaba D.K. Ojo, K.K. and Opintan J.A. (2007). Growing Problem of Multidrug-Resistant Enteric Pathogens in Africa. Emerg. Infect. Dis., 13(11): 1640-1646. https://doi.org/10.3201/eid1311.070674
- Okonko, I.O., Soleye, F.A., Amusan, T.A., Ogun, A.A., Ogunnusi, T.A. and Ejembi, J. (2009). Incidence of Multi-Drug Resistance (MDR) Organisms in Abeokuta, Southwestern Nigeria. Global J. Pharmacol., 3(2): 69-80.
- Olatoye, I.O. and Ehimowo, A.A. (2010). Oxytetracycline residues in edible tissues of cattle slaughtered in Akure, Nigeria. Nig. Vet. J. 31 (2): 93-102.
- Özdemir, N. (2003). Kimi uzunetkili (LA) veterinertetrasiklinformülasyonlarınınbiyoyararlanımvebiyoeşdeğerlilikyönündenincelenmesi [PhD Thesis]. Istanbul: İstanbul ÜniversitesiSağlıkBilimleriEnstitüsü.
- Reidenberg, M.M. and Conner, B.A. (2001). Counterfeit and substandard drugs. Clin. Pharmacol. Ther.,69: 189–93. https://doi.org/10.1067/mcp.2001.114672
- Rimoy, G.H., Moshi, M.J. and Massele, A.Y. (2002). Comparative bioavailability of oral sugar-coated and plain formulation of chloroquine phosphate marketed in Tanzania. Trop. Doct.,32: 15–17. https://doi.org/10.1177/004947550203200108
- Saba, A.B., Olatoye, I.O. and Oridupa, O.A. (2012). Spectrophotometric Analysis of Oxytetracycline Brands Available Over the Counter for Veterinary Use in South Western Nigeria. Nig. Vet. J.,33(3): 533-530
- Safila, N. (2014). Analytical Determination of Lisinopril Using UV Spectrophotometer and HPLC: An Overview. Mod. Chem. Appl.,2: 137. https://doi.org/10.4172/2329-6798.1000137
- Shakoor, O., Taylor, R.B. and Behrens, R.H. (1997). Assessment of the incidence of substandard drug in developing countries. Trop. Med. Int. Health, 2(9): 839-845.
- Shivachandra, S., Viswas, K. and Kumar, A. (2011). A review of hemorrhagicsepticemia in cattle and buffalo. Anim. Health Res. Rev. 12: 67-82. https://doi.org/10.1017/s146625231100003x
- Zhang, Q., Sahin, O., McDermott, P.F. and Payot, S. (2006). Fitness of antimicrobial resistant Campylobacter and Salmonella. Microbes Infect.,8:1972-1978. https://doi.org/10.1016/j.micinf.2005.12.031
References
Abubakar, A. (2017). "Origin and meaning of Maiduguri." Retrieved from www.researchgate.net/publication/318215165_Origin_and_Meaning_of_Maiduguri.
Aktas, I. and Yarsan, E. (2017). Pharmacokinetics of Conventional and Long-Acting Oxytetracycline Preparations in Kilis Goat. Front. Vet. Sci. 4, 229.
Aliyu, E.H., Nma, B.A., Suleiman, Y. and Muhammed, K.Y. (2018) A survey of antimicrobial usage by animal health practitioners in Niger State. In: A Paper Presentation at the 55th Annual Congress of the Nigerian Veterinary Medical Association at Usmanu Danfodio University Sokoto, 22nd -27th, October, 2018
Audu, S.A, Ahmed, A, Taiwo, A.E, SanI, M, Ojuolape, A.R., Sani, A.S., Bukola, A.R. and Mohammed, I. (2012). Comparative Quantitative Analysis of Different Brands of 300mg Aspirin Tablet Marketed in Maiduguri Metropolitan Council (MMC). IJPSR 3(8): 426-410
Boxall, A.B.A, Fogg, L.A., Blackwell, P.A., Kay, P., Pemberton, E.J. and Croxford, A. (2004). Veterinary medicines in the environment. Rev. Environ. Contam. Toxicol., 180. 1-91
Brunton, S., Carmichael, B., Fitzgerald, M., Liu, H., Varon, J. and Weiland, D. (2005). Community-acquired bacterial respiratory tract infections: consensus recommendations. J. Fam. Pract., 54(3): 255-262, PMID: 15755379.
Cantrell, L., Suchard, J. R., Wu, A. and Gerona, R.R. (2012). Stability of active ingredients in long-expired prescription medications. Arch. Intern. Med., 172(21):1685–1687. https://doi.org/10.1001/archinternmed.2012.4501
Daniel, N., Malgwi, K.D., Umaru, B., Omeh, I.J. and Sanya, L. (2021). A survey of the usage (frequency and pattern) of antibiotics at the University of Maiduguri Veterinary Teaching Hospital,Maiduguri, Borno State, Nigeria. Vet. World, 14(11): 2941-2946. www.doi.org/10.14202/vetworld.2021.2941-2946
Dina, O.A. and Arowolo R.O.A. (1991): Some considerations on veterinary drug use and supply in Nigeria. Re. d’Elevage Med. Vet. Pays Tropicaux, 44, 29-31.
Erb, A., Sturmer, T., Marre, R. and Brenner, H. (2007). Prevalence of antibiotic resistance in Escherichia coli: overview of geographical, temporal, and methodological variations. Eur J Clin Microbiol Infect Dis, 26:8390. https://doi.org/10.1007/s10096-006-0248-2
Ezenduka, E.V., Oboegbulem, S.I., Nwanta, J.A. and Onunkwo, J.I. (2011). Prevalence of antimicrobial residues in raw table eggs from farms and retail outlets in Enugu State, Nigeria. Trop. Anim. Health Prod., 43 (3): 557-559. https://doi.org/10.1007/s11250-010-9730-z
Geidam, Y.A., Ibrahim, U.I., Grema, H.A., Sanda, K.A., Suleiman, A. and Mohzo, D.L. (2012) Patterns of antibiotic sales by drug stores and usage in poultry farms: A questionnaire-based survey in Maiduguri, Northeastern Nigeria. J. Anim. Vet. Adv., 11(16): 2852-2855.
Idowu, F., Junaid, K., Paul, A., Gabriel, O., Paul, A., Sati, N., Maryam, M. and Jarlath, U., (2010). Antimicrobial screening of commercial eggs and determination of tetracycline residue using two microbiological methods. Int. J. Poultry Sci., 9 (10): 959-962.
Islam, M.S., Rahman, M.M., Bhuyian, M.M.U., Shamsuddin, M. and Islam, M.T. 2016. Efficacy of oxytetracycline, amoxicillin, sulfamethoxazole and trimethoprim, and tylosin for the treatment of bacterial diseases in cattle and goats. Bangladesh J. Vet. Med. 14(1): 47-51.
Kaya, S. (2007). Kemoterapötikler. 2nd ed. In: Kaya S, Pirinçci İ, Bilgili A, editors. VeterinerFarmakoloji. (Vol. 2), Ankara: Medisan Press. pp. 327–488.
Keita, A., Pommier, P., Pagot, E., Couper, A. and Cromie, L. (2007). A combination oxytetracycline/flunixin treatment of respiratory infections in cattle. Rev. Méd. Vét., 158: 86-91.
Kovacs, S., Hawes, S.E., Maley, S.N., Mosites, E., Wong, L. and Stergachis, A. (2014) Technologies for detecting falsified and substandard drugs in low and middle-income countries. PLoS One, 9(3):e90601. https://doi.org/10.1371/journal.pone.0090601
Levy, S. B. (1992). Active efflux mechanisms for antimicrobial resistance. Antimicrob. Agents Chemother., 36: 695–703. https://doi.org/10.1128/aac.36.4.695
Levy, S.B. and Marshall, B. (2004). Antibacterial resistance worldwide: causes, challenges and responses. Nat Med, 10(12 Suppl): S122-129. https://doi.org/10.1038/nm1145
Merey, H. A., Abd-Elmonem, M. S., Nazlawy, H. N. and Zaazaa, H. E. (2017). Spectrophotometric Methods for Simultaneous Determination of Oxytetracycline HCl and Flunixin Meglumine in Their Veterinary Pharmaceutical Formulation. J. Anal. Methods Chem.: 2321572. https://doi.org/10.1155/2017/2321572
Moken, MC. (2003). Fake pharmaceuticals: how they and relevant legislation or lack thereof contribute to consistently high and increasing drug prices. Am. J Law Med, 29: 525–542. PMID: 15119247
Nazerali, H. and Hogerzeil, H.V. (1998). The quality and stability of essential drugs in rural Zimbabwe: controlled longitudinal study. BMJ, 17(7157):512-513. https://doi.org/10.1136/bmj.317.7157.512
Ojo, O.E., Oyekunle, M.A., Ogunleye, A.O. and Otesile, E.B. (2009). Escherichia coli, O157:H7 in Food animals in part of south-western Nigeria: Prevalence and in vitro antimicrobial susceptibility. Trop. Vet. 26 (3and4): 23-30
Okeke, I.N., and Laxminarayan, R., Bhutta, Z.A., Duse, A.G., Jenkins, P., O'Brien, T.F., Pablos-Mendez, A. and Klugman, K.P. (2005). Antimicrobial resistance in developing countries. Part I: recent trends and current status. Lancet Infect. Dis.,5(8), 481–493. https://doi.org/10.1016/s1473-3099(05)70189-4
Okeke, I.N., Aboderin, O.A., Byarugaba D.K. Ojo, K.K. and Opintan J.A. (2007). Growing Problem of Multidrug-Resistant Enteric Pathogens in Africa. Emerg. Infect. Dis., 13(11): 1640-1646. https://doi.org/10.3201/eid1311.070674
Okonko, I.O., Soleye, F.A., Amusan, T.A., Ogun, A.A., Ogunnusi, T.A. and Ejembi, J. (2009). Incidence of Multi-Drug Resistance (MDR) Organisms in Abeokuta, Southwestern Nigeria. Global J. Pharmacol., 3(2): 69-80.
Olatoye, I.O. and Ehimowo, A.A. (2010). Oxytetracycline residues in edible tissues of cattle slaughtered in Akure, Nigeria. Nig. Vet. J. 31 (2): 93-102.
Özdemir, N. (2003). Kimi uzunetkili (LA) veterinertetrasiklinformülasyonlarınınbiyoyararlanımvebiyoeşdeğerlilikyönündenincelenmesi [PhD Thesis]. Istanbul: İstanbul ÜniversitesiSağlıkBilimleriEnstitüsü.
Reidenberg, M.M. and Conner, B.A. (2001). Counterfeit and substandard drugs. Clin. Pharmacol. Ther.,69: 189–93. https://doi.org/10.1067/mcp.2001.114672
Rimoy, G.H., Moshi, M.J. and Massele, A.Y. (2002). Comparative bioavailability of oral sugar-coated and plain formulation of chloroquine phosphate marketed in Tanzania. Trop. Doct.,32: 15–17. https://doi.org/10.1177/004947550203200108
Saba, A.B., Olatoye, I.O. and Oridupa, O.A. (2012). Spectrophotometric Analysis of Oxytetracycline Brands Available Over the Counter for Veterinary Use in South Western Nigeria. Nig. Vet. J.,33(3): 533-530
Safila, N. (2014). Analytical Determination of Lisinopril Using UV Spectrophotometer and HPLC: An Overview. Mod. Chem. Appl.,2: 137. https://doi.org/10.4172/2329-6798.1000137
Shakoor, O., Taylor, R.B. and Behrens, R.H. (1997). Assessment of the incidence of substandard drug in developing countries. Trop. Med. Int. Health, 2(9): 839-845.
Shivachandra, S., Viswas, K. and Kumar, A. (2011). A review of hemorrhagicsepticemia in cattle and buffalo. Anim. Health Res. Rev. 12: 67-82. https://doi.org/10.1017/s146625231100003x
Zhang, Q., Sahin, O., McDermott, P.F. and Payot, S. (2006). Fitness of antimicrobial resistant Campylobacter and Salmonella. Microbes Infect.,8:1972-1978. https://doi.org/10.1016/j.micinf.2005.12.031