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Abstract
Haematological parameters are good indicators of the physiological status used by equine practitioners for health assessment. The aim of this study was to evaluate the influences of sex, age, breed and activity on haematological parameters of apparently healthy horses from three South-western states in Nigeria. Blood samples from 100 apparently healthy horses with age ranges from 1 to 18 years old, both sexes, mixed breeds, and engaged in different activities were obtained for hematological analysis and erythrocyte osmotic fragility test. The results showed that the haematological values are comparable with normal reference values for horses. The age, sex, breed and activities had exerted some effect on the hematological values with significantly (p<0.05) higher hemoglobin concentration (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and white blood cells count (WBC) in the stallions than mares. Older horses had significantly (p<0.05) lower Hb and MCH values than younger ones. The Nigerian Local horse (indigenous) and the cross breed had significantly (p<0.05) higher Hb and MCHC. Horse used for recreation activities had significantly (p<0.05) higher values when compared with other horse activities. Similarly, horse activities influenced the erythrocyte osmotic fragility. In conclusion, this study showed that the haemogram values for horses from South-western Nigeria are within the acceptable reference values for horses. Some effect of sex, age, breed and activities were observed on the haematological parameters of the horse with stallions, the indigenous, their cross breed and those engaged in recreational activities having significantly higher Hb, MCH, MCHC and WBC values.
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Copyright (c) 2025 A. M. Mayaki , Y. I. Likita, O. A. Alaba, Adah Osereime, O. O. Akinniyi , T. F. Ajani , O. I. Azeez, A. A. Oyagbemi, D. O. Omoniwa , O. G. Ohore, T. O. Omobowale (Author)

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References
- Adamu, L., Noraniza, M.A., Rasedee, A. and Bashir, A. (2013). Effect of age and performance on physical, haematological , and biochemical parameters in endurance horses. J. Equine. Vet. Sci., 33: 415–420.
- Adedokun, R.A.M., Olaogun, S.C. and Alaba, B.A. (2023). Haematological and Biochemical Profile of Apparently Healthy Horses in Ibadan, Nigeria. AJVS. 77 (1): 156-164.
- Adenkola, A.Y. and Ayo, J.O. (2009). Effect of road transportation on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season in Zaria, Nigeria. JCAB., 3(1): 4-8.
- Akinniyi, O.O., Okuneye, D.T., Alaba, B.A., Banwo, O.G., Koleosho, S.A., Jeremiah, O.T. and Omobowale, T.O. (2025). Comparative analysis of clinical, haematological and serum biochemical profiles in polo horses and their sedentary counterparts. Equine Vet. Edu. (in press). https://doi.org/10.1111/eve.14083
- Azeez, O.I., Olayemi, F.O. and Olanrewaju, J. R. (2011). Age and Sex Influences on the Haematology and Erythrocyte Osmotic Fragility of the Nigerian Turkey. Res. J. Vet. Sci., 4: 43 - 49.
- Azeez, O.I. Oyewale, J.O and Okunola, O. O. (2009). Erythrocyte Osmotic Fragility of Nera Black Fowls of Two-Age Groups. Int. J. Poul. Sci., 8(2): 192-194.
- Bachman, E., Travison, T.G., Basaria, S., Davda, M.N., Gui, W., Li, M., Westfall, J.C., Bae, H., Gordeuk, V. and Bhasin, S. (2014). Testosterone induces erythrocytosis via increased erythropoietin and suppressed hepcidin: evidence for a new erythropoietin/ hemoglobin set point. J. Gerontol. A. Biol. Sci. Med. Sci., 69:725-735.
- Basiru, A., Ishola, A. O., Sanusi, F. and Olaifa, F.H. (2022). Influence of Age on Haematology, Serum Biochemistry and Lipid Profile of Stallions in Ilorin, Nigeria. Sahel J. Vet. Sci., 19(2): 21-25
- Brzezińska-Ślebodzińska, E. (2001). Erythrocyte osmotic fragility test as the measure of defence against free radicals in rabbits of different age. Acta Vet. Hung., 49(4): 413-419.
- Buendia, A., Teng, K.T- Y., Camino, E., Dominguez, L. and Cruz-Lopez, F. (2021). Influence of multiple factors on hematologic reference intervals in horses residing in livery yards in Spain. Vet Clin Pathol., 50(2):273-277. https://doi. org/10.1111/vcp.12965
- Burlikowska, K., Bogusławska-Tryk, M., Szymeczko. R. and Piotrowska, A. (2015). Haematological and biochemical blood parameters in horses used for sport and recreation. J. Cent. Eur. Agric., 16(4): 370-382.
- Clark, M.A., Kanjee, U., Rangel, G.W., Chery, L., Mascarenhas, A., Gomes, E., Rathod, P.K., Brugnara, C., Ferreira, M.U. and Duraisingh, M.T., (2021). Plasmodium vivax infection compromises reticulocyte stability. Nat. Commun., 12 (1): 1-11.
- Coles, E.H. (1986). Veterinary Clinical Pathology. 4th ed. W.B. Saunders, Philadelphia.
- Dent, A.A. and Hendricks, B.L. (2001). International encyclopedia of horse breeds, Norman: University of Oklahoma Press.
- Ebge-Nwiyi, T.N., Kalu, N.A. and Naphtali, C. (2012). Preliminary studies on some haematological and serum biochemical parameters of apparently healthy adult horses in Maiduguri, Nigeria. Afri. J. Biomed. Res., 15(1): 49-53.
- Ememe, M.U., Tchokote, E.Y. and Akpuoye, O.C. (2015). Studies on haematological parameters in horses located in Port Harcourt Nigeria. Merit Res. J. Biochem. Bioinformatics., 3(1): 1-4.
- Galleano, M. and Puntarulo, S., (1995). Role of antioxidants on the erythrocyte resistance to lipid peroxidation after acute iron overload in rats. Biochimica et Biophysica Acta., 1271 (2-3): 321-326.
- Garba, U. M. (2006). Babesia in royal horses in Niger state. MSc thesis presented to Faculty of Veterinary Medicine, A.B.U., Zaria.
- Hanzawa, K. and Watanabe, S. (2000). Changes in Osmotic Fragility of Erythrocytes during Exercise in Athletic Horses. J. Equine. Sci., 11(3): 51-61.
- Hassan, A.Z and Hassan, F.B. (2003). An Introduction to Veterinary Practice. 1st edn. A.B.U. Zaria press Ltd. pp. 64.
- Ihedioha, J.I., and Agina, O.A. (2014). Haematological profile of Nigerian horses in Obollo Afor Enugu State. J. Sci., 4(1): 1-8.
- Inoue, Y., Ono, T., Hisaeda, K., Yamada, Y., Hata, A., Miyama, T.S., Shibano, K., Ohzawa, E., Kitagawa, H. and Iwata, E. (2022). Relationships between the age and blood test results or body sizes in Noma horses. J. Equine. Sci., 33(2): 27-30.
- Isović, J., Čamo, D., Ćutuk, R. and Zahirović, A, (2023). The influence of various physical activity on haematological and biochemical parameters in draft and sport horses. International J. Vet. Sci., 12(5): 708-714. https://doi.org/10.47278/journal.ijvs/2023.014
- Larsson, J., Pilborg, P.H., Johansen, M., Christophersen, M.T., Holte, A., Roepstorff, L., Olsen, L.H. and Harrison, A. P. (2013). Physiological Parameters of Endurance Horses Pre-Compared to Post-Race, Correlated with Performance: A Two Race Study from Scandinavia. ISRN Vet. Sci. 2013: 684353.
- Lee, Y-W., Shim, S-T., Song, H-E., Hwang, H-S., Seo, J-P. and Lee, K-K. (2018). The Correlation of Racing Performance with Pre-Race Blood Values in Thoroughbred Horses. J Vet Clin., 35(5): 190-194.
- Masko, M., Domino, M., Jasinski, T., and Witkowska-Piłaszewicz, O. (2021). The physical activity-dependent haematological and biochemical changes in school horses in comparison to blood profiles in endurance and race horses. Animals., 11: 1128. https://doi.org/ 10.3390/ani11041128.
- Massányi, M., Halo Jr. M., Massányi, P., Mlyneková, E., Greń, A., Formicki, G. and Halo, M. (2022). Changes in haematological and biochemical parameters in blood serum of horses during exposition to workload stress. Helyon., 8(12): e12241. https://doi.org/10.1016/j.heliyon.2022.e12241
- Mayaki, A.M., Mera, U.M. and Talabi, A.O. (2018). Prevalence of equine colic and its associated risk factors in Sokoto Metropolis, Nigeria. Vom J. Vet. Sci., 13: 44-51.
- Mazaheri, P., Nadkarni, G., Lowe, E., Hines, P., Vuica, M., Griffin, M. and Resar, L. M. (2010). Ghosal hematodiaphyseal dysplasia: a rare cause of a myelophthisic anemia. Pediatri. Blood Cancer., 55(6): 1187-1190.
- Munoz, A., Riber, C., Trigo, P. and Castejon, F. (2012). Age- and gender-related variations in hematology, clinical biochemistry, and hormones in Spanish fillies and colts. Res Vet Sci., 93: 943-949.
- Neuberg-Zuchowicz, K. and Geringer de Oedenberg, H. (2011). Changes in haematological parameters of show jumping horses during yearly training cycle. Medycyna Wet. 67(11): 765-769.
- Ono, T., Yamada, Y., Hata, A., Shimokawa Miyama, T., Shibano, K., Iwata, E., Ohzawa, E. and Kitagawa, H. (2019). Reference values of haematological and blood biochemical parameters for the Noma horse. J. Equine Sci., 30(3): 69–73. https://doi.org/10.1294/jes.30.69.
- Oyewale, J.O. (1992). Effects of temperature and pH on osmotic fragility of erythrocytes of the domestic fowl (Gallus domesticus) and guinea fowl (Numida maleagridis). Res. Vet. Sci., 52: 1 – 4.
- Ramkumar, K.M., Vijayakumar, R.S., Vanitha, P., Suganya, N., Manjula, C., Rajaguru, P., Sivasubramanian, S. and Gunasekaran, P. (2014). Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats. Hum. Exp. Toxicol., 33 (6): 638-649.
- RIM (1992). Nigerian National Livestock Resource Survey. Report by Resource Inventory and management Limited (RIM) to FDL and PCS, Abuja, Nigeria.Vol.6.
- Satue, K., Blanco, O. and Munoz, A. (2009). Age-related differences in the haematological profile of Andalusian brood mares of Carthusian strain. Vet Med (Praha), 54:175-182.
- Satue, K., Hernadez, A., Lorente, C., Fazio, E. and Medica, P. (2020). Age-and sex-related modifications of hematology in Spanish purebred horse. J. Equine. Vet. Sci., 93: 103219.
- Sawesi, O.K., Alteab, A.A., Elbaz,A.K., Mahmoud,A.S., Milad, K.K., Duro, E.M. and Bennuor, E.M. (2023). Haematological reference values of horses in Western Libya and their relationship to breed, age, and management. Open Vet. J., 13(12): 1696–1707.
- Schalm, O.W., Jain, N.C. and Carrol, E.J. (1975). Veterinary Haematology. 3rd ed. Philadelphia: Lea & Febiger. pp. 197-199.
- Silva Rua M.A., Vega, W.H.O., Bartholazzi Junior, A., Matos, L.F. and Quirino, C.R. (2024). Haematological and biochemical reference values for the Brazilian Pony breed. Arch. Latinoam. Prod. Anim. 32 (1): 1-7.
- Takasu, M., Nagatani, N., Tozaki, T., Kakoi, H., Maeda, M., Murase, T., and Mukoyama, H. (2013). Haematological and biochemical reference values for the endangered Kiso horse. J. Equine Sci., 24 (4): 75-78. https://doi.org/10.1294/jes.24.75.
- Udeh, N.E., Orji, E., Madubuike, A.J., Ojiako, C., Okite, N. and Felix, G.C. (2021). Effect of season, age and sex on haematology and serum biochemistry of sport horses. JoSVAS, 1(2): 140-144. http://doi.org/10.54328/covm.josvas.2021.032
- Witkowska-Piłaszewicz, O., Masko, M., Domino, M. and Winnicka, A. (2020). Infrared thermography correlates with lactate concentration in blood during race training in horses. Animals., 10(11): 2072.
- Yaqub, L.S., Mshelia, W.P. and Ayo, J.O. (2014). Erythrocyte osmotic fragility and haematological responses of horses administered ascorbic acid and exposed to road transportation. J. Equine Vet. Sci., 34 (11-12): 1324-1328.
- Yaqub, L.S., Ayo. J.O., Habibu, B., Kawu, M.U. and Rekwot, P.I. (2021). Haematological responses and erythrocyte osmotic fragility in pregnant Yankasa ewes and their lambs. Small. Rumin. Res.,198: 106352
References
Adamu, L., Noraniza, M.A., Rasedee, A. and Bashir, A. (2013). Effect of age and performance on physical, haematological , and biochemical parameters in endurance horses. J. Equine. Vet. Sci., 33: 415–420.
Adedokun, R.A.M., Olaogun, S.C. and Alaba, B.A. (2023). Haematological and Biochemical Profile of Apparently Healthy Horses in Ibadan, Nigeria. AJVS. 77 (1): 156-164.
Adenkola, A.Y. and Ayo, J.O. (2009). Effect of road transportation on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season in Zaria, Nigeria. JCAB., 3(1): 4-8.
Akinniyi, O.O., Okuneye, D.T., Alaba, B.A., Banwo, O.G., Koleosho, S.A., Jeremiah, O.T. and Omobowale, T.O. (2025). Comparative analysis of clinical, haematological and serum biochemical profiles in polo horses and their sedentary counterparts. Equine Vet. Edu. (in press). https://doi.org/10.1111/eve.14083
Azeez, O.I., Olayemi, F.O. and Olanrewaju, J. R. (2011). Age and Sex Influences on the Haematology and Erythrocyte Osmotic Fragility of the Nigerian Turkey. Res. J. Vet. Sci., 4: 43 - 49.
Azeez, O.I. Oyewale, J.O and Okunola, O. O. (2009). Erythrocyte Osmotic Fragility of Nera Black Fowls of Two-Age Groups. Int. J. Poul. Sci., 8(2): 192-194.
Bachman, E., Travison, T.G., Basaria, S., Davda, M.N., Gui, W., Li, M., Westfall, J.C., Bae, H., Gordeuk, V. and Bhasin, S. (2014). Testosterone induces erythrocytosis via increased erythropoietin and suppressed hepcidin: evidence for a new erythropoietin/ hemoglobin set point. J. Gerontol. A. Biol. Sci. Med. Sci., 69:725-735.
Basiru, A., Ishola, A. O., Sanusi, F. and Olaifa, F.H. (2022). Influence of Age on Haematology, Serum Biochemistry and Lipid Profile of Stallions in Ilorin, Nigeria. Sahel J. Vet. Sci., 19(2): 21-25
Brzezińska-Ślebodzińska, E. (2001). Erythrocyte osmotic fragility test as the measure of defence against free radicals in rabbits of different age. Acta Vet. Hung., 49(4): 413-419.
Buendia, A., Teng, K.T- Y., Camino, E., Dominguez, L. and Cruz-Lopez, F. (2021). Influence of multiple factors on hematologic reference intervals in horses residing in livery yards in Spain. Vet Clin Pathol., 50(2):273-277. https://doi. org/10.1111/vcp.12965
Burlikowska, K., Bogusławska-Tryk, M., Szymeczko. R. and Piotrowska, A. (2015). Haematological and biochemical blood parameters in horses used for sport and recreation. J. Cent. Eur. Agric., 16(4): 370-382.
Clark, M.A., Kanjee, U., Rangel, G.W., Chery, L., Mascarenhas, A., Gomes, E., Rathod, P.K., Brugnara, C., Ferreira, M.U. and Duraisingh, M.T., (2021). Plasmodium vivax infection compromises reticulocyte stability. Nat. Commun., 12 (1): 1-11.
Coles, E.H. (1986). Veterinary Clinical Pathology. 4th ed. W.B. Saunders, Philadelphia.
Dent, A.A. and Hendricks, B.L. (2001). International encyclopedia of horse breeds, Norman: University of Oklahoma Press.
Ebge-Nwiyi, T.N., Kalu, N.A. and Naphtali, C. (2012). Preliminary studies on some haematological and serum biochemical parameters of apparently healthy adult horses in Maiduguri, Nigeria. Afri. J. Biomed. Res., 15(1): 49-53.
Ememe, M.U., Tchokote, E.Y. and Akpuoye, O.C. (2015). Studies on haematological parameters in horses located in Port Harcourt Nigeria. Merit Res. J. Biochem. Bioinformatics., 3(1): 1-4.
Galleano, M. and Puntarulo, S., (1995). Role of antioxidants on the erythrocyte resistance to lipid peroxidation after acute iron overload in rats. Biochimica et Biophysica Acta., 1271 (2-3): 321-326.
Garba, U. M. (2006). Babesia in royal horses in Niger state. MSc thesis presented to Faculty of Veterinary Medicine, A.B.U., Zaria.
Hanzawa, K. and Watanabe, S. (2000). Changes in Osmotic Fragility of Erythrocytes during Exercise in Athletic Horses. J. Equine. Sci., 11(3): 51-61.
Hassan, A.Z and Hassan, F.B. (2003). An Introduction to Veterinary Practice. 1st edn. A.B.U. Zaria press Ltd. pp. 64.
Ihedioha, J.I., and Agina, O.A. (2014). Haematological profile of Nigerian horses in Obollo Afor Enugu State. J. Sci., 4(1): 1-8.
Inoue, Y., Ono, T., Hisaeda, K., Yamada, Y., Hata, A., Miyama, T.S., Shibano, K., Ohzawa, E., Kitagawa, H. and Iwata, E. (2022). Relationships between the age and blood test results or body sizes in Noma horses. J. Equine. Sci., 33(2): 27-30.
Isović, J., Čamo, D., Ćutuk, R. and Zahirović, A, (2023). The influence of various physical activity on haematological and biochemical parameters in draft and sport horses. International J. Vet. Sci., 12(5): 708-714. https://doi.org/10.47278/journal.ijvs/2023.014
Larsson, J., Pilborg, P.H., Johansen, M., Christophersen, M.T., Holte, A., Roepstorff, L., Olsen, L.H. and Harrison, A. P. (2013). Physiological Parameters of Endurance Horses Pre-Compared to Post-Race, Correlated with Performance: A Two Race Study from Scandinavia. ISRN Vet. Sci. 2013: 684353.
Lee, Y-W., Shim, S-T., Song, H-E., Hwang, H-S., Seo, J-P. and Lee, K-K. (2018). The Correlation of Racing Performance with Pre-Race Blood Values in Thoroughbred Horses. J Vet Clin., 35(5): 190-194.
Masko, M., Domino, M., Jasinski, T., and Witkowska-Piłaszewicz, O. (2021). The physical activity-dependent haematological and biochemical changes in school horses in comparison to blood profiles in endurance and race horses. Animals., 11: 1128. https://doi.org/ 10.3390/ani11041128.
Massányi, M., Halo Jr. M., Massányi, P., Mlyneková, E., Greń, A., Formicki, G. and Halo, M. (2022). Changes in haematological and biochemical parameters in blood serum of horses during exposition to workload stress. Helyon., 8(12): e12241. https://doi.org/10.1016/j.heliyon.2022.e12241
Mayaki, A.M., Mera, U.M. and Talabi, A.O. (2018). Prevalence of equine colic and its associated risk factors in Sokoto Metropolis, Nigeria. Vom J. Vet. Sci., 13: 44-51.
Mazaheri, P., Nadkarni, G., Lowe, E., Hines, P., Vuica, M., Griffin, M. and Resar, L. M. (2010). Ghosal hematodiaphyseal dysplasia: a rare cause of a myelophthisic anemia. Pediatri. Blood Cancer., 55(6): 1187-1190.
Munoz, A., Riber, C., Trigo, P. and Castejon, F. (2012). Age- and gender-related variations in hematology, clinical biochemistry, and hormones in Spanish fillies and colts. Res Vet Sci., 93: 943-949.
Neuberg-Zuchowicz, K. and Geringer de Oedenberg, H. (2011). Changes in haematological parameters of show jumping horses during yearly training cycle. Medycyna Wet. 67(11): 765-769.
Ono, T., Yamada, Y., Hata, A., Shimokawa Miyama, T., Shibano, K., Iwata, E., Ohzawa, E. and Kitagawa, H. (2019). Reference values of haematological and blood biochemical parameters for the Noma horse. J. Equine Sci., 30(3): 69–73. https://doi.org/10.1294/jes.30.69.
Oyewale, J.O. (1992). Effects of temperature and pH on osmotic fragility of erythrocytes of the domestic fowl (Gallus domesticus) and guinea fowl (Numida maleagridis). Res. Vet. Sci., 52: 1 – 4.
Ramkumar, K.M., Vijayakumar, R.S., Vanitha, P., Suganya, N., Manjula, C., Rajaguru, P., Sivasubramanian, S. and Gunasekaran, P. (2014). Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats. Hum. Exp. Toxicol., 33 (6): 638-649.
RIM (1992). Nigerian National Livestock Resource Survey. Report by Resource Inventory and management Limited (RIM) to FDL and PCS, Abuja, Nigeria.Vol.6.
Satue, K., Blanco, O. and Munoz, A. (2009). Age-related differences in the haematological profile of Andalusian brood mares of Carthusian strain. Vet Med (Praha), 54:175-182.
Satue, K., Hernadez, A., Lorente, C., Fazio, E. and Medica, P. (2020). Age-and sex-related modifications of hematology in Spanish purebred horse. J. Equine. Vet. Sci., 93: 103219.
Sawesi, O.K., Alteab, A.A., Elbaz,A.K., Mahmoud,A.S., Milad, K.K., Duro, E.M. and Bennuor, E.M. (2023). Haematological reference values of horses in Western Libya and their relationship to breed, age, and management. Open Vet. J., 13(12): 1696–1707.
Schalm, O.W., Jain, N.C. and Carrol, E.J. (1975). Veterinary Haematology. 3rd ed. Philadelphia: Lea & Febiger. pp. 197-199.
Silva Rua M.A., Vega, W.H.O., Bartholazzi Junior, A., Matos, L.F. and Quirino, C.R. (2024). Haematological and biochemical reference values for the Brazilian Pony breed. Arch. Latinoam. Prod. Anim. 32 (1): 1-7.
Takasu, M., Nagatani, N., Tozaki, T., Kakoi, H., Maeda, M., Murase, T., and Mukoyama, H. (2013). Haematological and biochemical reference values for the endangered Kiso horse. J. Equine Sci., 24 (4): 75-78. https://doi.org/10.1294/jes.24.75.
Udeh, N.E., Orji, E., Madubuike, A.J., Ojiako, C., Okite, N. and Felix, G.C. (2021). Effect of season, age and sex on haematology and serum biochemistry of sport horses. JoSVAS, 1(2): 140-144. http://doi.org/10.54328/covm.josvas.2021.032
Witkowska-Piłaszewicz, O., Masko, M., Domino, M. and Winnicka, A. (2020). Infrared thermography correlates with lactate concentration in blood during race training in horses. Animals., 10(11): 2072.
Yaqub, L.S., Mshelia, W.P. and Ayo, J.O. (2014). Erythrocyte osmotic fragility and haematological responses of horses administered ascorbic acid and exposed to road transportation. J. Equine Vet. Sci., 34 (11-12): 1324-1328.
Yaqub, L.S., Ayo. J.O., Habibu, B., Kawu, M.U. and Rekwot, P.I. (2021). Haematological responses and erythrocyte osmotic fragility in pregnant Yankasa ewes and their lambs. Small. Rumin. Res.,198: 106352