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Abstract
This study evaluated hematological and serum biochemical profiles of West African Dwarf (WAD) goats across different physiological stages; juveniles, nursing mothers, and pregnant does, reared under identical intensive management. Fifteen healthy goats (n = 5 per group) were monitored over six weeks in Ibadan, Nigeria. Blood samples were analyzed weekly for packed cell volume (PCV), hemoglobin (Hb), red and white blood cell counts (RBC, WBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), total protein (TP), albumin, globulin, liver enzymes (AST, ALP), blood urea nitrogen (BUN), creatinine, and glucose. Pregnant goats exhibited significantly higher PCV (33.8 ± 4.2%), Hb (12.1 ± 1.2 g/dL), WBC (13.2 ± 2.5 × 10⁹/L), TP (8.1 ± 0.9 g/dL), AST (329.9 ± 45.2 U/L), ALP (246.8 ± 35.7 U/L), and BUN (26.2 ± 3.4 mg/dL) compared to juveniles and nursing mothers (p < 0.05). RBC counts (10.9–11.5 × 10¹²/L), MCV (19.9–20.1 fL), MCH (5.9–6.4 pg), creatinine (1.2–1.8 mg/dL), and glucose (86.1–90.2 mg/dL) remained relatively stable across groups. Our results highlight clear stage-dependent hematological and biochemical adaptations during pregnancy and early life emphasizing the inadequacy of generalized adult reference intervals and the need for physiological-stage-specific thresholds in WAD goats. Adoption of cohort-specific reference values will enhance diagnostic precision, support targeted nutritional planning, and improve herd health management under intensive systems.
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Copyright (c) 2025 A. K. Olaifa, A. Ariyibi, A. A. Usman, O. S. Olaifa, S. Balogun (Author)

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References
- Chen, J., Li, S., and Zhao, H. (2022). Gestational leukocytosis and neutrophil percentages in dairy goats: Breed specific immune modulation. Veterinary Immunology and Immunopathology, 238, 110343. https://doi.org/10.1016/j.vetimm.2021.110343
- Daramola, J. O., Adeyemo, O. K. and Adu, I. F. (2005). Factors affecting the hematology of animals. Nigerian Journal of Animal Production, 32(2), 165-170.
- Gomez Pérez, A., Fernández Llario, P., and Carro, M. D. (2015). Creatinine stability across reproductive stages in Murciano Granadina does. Small Ruminant Research, 131, 112–118. https://doi.org/10.1016/j.smallrumres.2015.04.007
- Habibu, B., Makun, H. J., Yaqub, L. S., Buhari, H. U., Aluwong, T., and Kawu, M. U. (2017). Comparative evaluation of haematological parameters and erythrocyte membrane stability in pregnant and lactating goats in different seasons of tropical Savannah. Theriogenology, 99, 30–35. https://doi.org/10.1016/j.theriogenology.2017.05.004
- Li, Y., Zhang, L., and Wang, X. (2020). Comparative analysis of alkaline phosphatase activity in pregnant and non pregnant goats in a temperate climate. Animal Reproduction Science, 210, 106223. https://doi.org/10.1016/j.anireprosci.2019.106223
- Liotta, L., Bionda, A., Fauci, D. L., Quartuccio, M., Visalli, R. and Fazio, E. (2021). Steroid hormonal endpoints in goats carrying single or twin fetuses reared in semi-extensive systems. Archives Animal Breeding, 64(2), 467-474. https://doi.org/10.5194/aab-64-467-2021
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- Mbassa, G. K., and Poulsen, J. S. (1991b). Influence of pregnancy, lactation and environment on some clinical chemical reference values in Danish landrace dairy goats (Capra hircus) of different parity—II. Plasma urea, creatinine, bilirubin, cholesterol, glucose and total serum proteins. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 100(2), 423–431. https://doi.org/10.1016/0305-0491(91)90396-U
- Muayad, T.A., Haniza, M.Z.H., Husni, I. and Tawang, A. (2018). Haematological values of apparently healthy indigenous goats in Malaysia: A comparative study. Indian Journal of Animal Research, 52(12), 1701-1704.
- Noakes DM, Parkinson TJ, England GCW. Veterinary Reproduction and Obstetrics. 10th ed. Saunders; 2009.
- Okwelum, N., Mustapha, W. A. W. and Oluwatosin, B. O. (2020). Haematology and serum biochemical profile of Kalahari Red goats reared in the hot humid southwest Nigeria. Nigerian Journal of Animal Production, 45(5), 192-198. https://doi.org/10.51791/njap.v45i5.308
- Onasanya, G. O., Oke, F. O., Sanni, T. M. and Muhammad, A. I. (2015). Parameters influencing haematological, serum and biochemical references in livestock animals under different management systems. Open Journal of Veterinary Medicine, 5(8), 181-189. https://doi.org/10.4236/ojvm.2015.58025
- Oseni, S. O. and Ajayi, B. A. (2014). Phenotypic characterization and strategies for genetic improvement of WAD goats under backyard systems. Open Journal of Animal Sciences, 4(5), 253-262. https://doi.org/10.4236/ojas.2014.45032
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- Sandabe, U. K., Mustapha, A. R., and Sambo, E. Y. (2004a). Effect of pregnancy on some biochemical parameters in Sahel goats in semi arid zones. Veterinary Research Communications, 28(4), 279–285. https://doi.org/10.1023/B:VERC.0000026655.69812.79
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- Sarangi, B. (2018). Hematological and serum biochemical parameters in different breeds of goats. Indian Journal of Veterinary Sciences and Biotechnology, 14(2), 112-125.
- Shittu, O. B., Okwelum, N., Famakinde, S. A., Odeyemi, A. J. and Toviesi, D. P. (2023). Haematological, serum biochemical and hormonal profile in West African Dwarf goats during pregnancy. Agricultura Tropica et Subtropica, 56(1), 50-57. https://doi.org/10.2478/ats-2023-0006
- Shittu, O. O., Amole, T. A., Okwelum, N., Odeyemi, J. A., Toviesi, D.P., Ojo, V. I. O., Oluwatosin, B. O., Smith, O. F. and Osinowo, O. A. (2016). Haematological and serum biochemical parameters of West African Dwarf and Kalahari Red goats in the humid tropics. Nigerian Journal of Animal Science, 2, 305-314.
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References
Chen, J., Li, S., and Zhao, H. (2022). Gestational leukocytosis and neutrophil percentages in dairy goats: Breed specific immune modulation. Veterinary Immunology and Immunopathology, 238, 110343. https://doi.org/10.1016/j.vetimm.2021.110343
Daramola, J. O., Adeyemo, O. K. and Adu, I. F. (2005). Factors affecting the hematology of animals. Nigerian Journal of Animal Production, 32(2), 165-170.
Gomez Pérez, A., Fernández Llario, P., and Carro, M. D. (2015). Creatinine stability across reproductive stages in Murciano Granadina does. Small Ruminant Research, 131, 112–118. https://doi.org/10.1016/j.smallrumres.2015.04.007
Habibu, B., Makun, H. J., Yaqub, L. S., Buhari, H. U., Aluwong, T., and Kawu, M. U. (2017). Comparative evaluation of haematological parameters and erythrocyte membrane stability in pregnant and lactating goats in different seasons of tropical Savannah. Theriogenology, 99, 30–35. https://doi.org/10.1016/j.theriogenology.2017.05.004
Li, Y., Zhang, L., and Wang, X. (2020). Comparative analysis of alkaline phosphatase activity in pregnant and non pregnant goats in a temperate climate. Animal Reproduction Science, 210, 106223. https://doi.org/10.1016/j.anireprosci.2019.106223
Liotta, L., Bionda, A., Fauci, D. L., Quartuccio, M., Visalli, R. and Fazio, E. (2021). Steroid hormonal endpoints in goats carrying single or twin fetuses reared in semi-extensive systems. Archives Animal Breeding, 64(2), 467-474. https://doi.org/10.5194/aab-64-467-2021
Mbassa, G. K., and Poulsen, J. S. (1991a). Influence of pregnancy, lactation and environment on haematological profiles in Danish landrace dairy goats (Capra hircus) of different parity. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 100(2), 403–412. https://doi.org/10.1016/0305-0491(91)90394-S
Mbassa, G. K., and Poulsen, J. S. (1991b). Influence of pregnancy, lactation and environment on some clinical chemical reference values in Danish landrace dairy goats (Capra hircus) of different parity—II. Plasma urea, creatinine, bilirubin, cholesterol, glucose and total serum proteins. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 100(2), 423–431. https://doi.org/10.1016/0305-0491(91)90396-U
Muayad, T.A., Haniza, M.Z.H., Husni, I. and Tawang, A. (2018). Haematological values of apparently healthy indigenous goats in Malaysia: A comparative study. Indian Journal of Animal Research, 52(12), 1701-1704.
Noakes DM, Parkinson TJ, England GCW. Veterinary Reproduction and Obstetrics. 10th ed. Saunders; 2009.
Okwelum, N., Mustapha, W. A. W. and Oluwatosin, B. O. (2020). Haematology and serum biochemical profile of Kalahari Red goats reared in the hot humid southwest Nigeria. Nigerian Journal of Animal Production, 45(5), 192-198. https://doi.org/10.51791/njap.v45i5.308
Onasanya, G. O., Oke, F. O., Sanni, T. M. and Muhammad, A. I. (2015). Parameters influencing haematological, serum and biochemical references in livestock animals under different management systems. Open Journal of Veterinary Medicine, 5(8), 181-189. https://doi.org/10.4236/ojvm.2015.58025
Oseni, S. O. and Ajayi, B. A. (2014). Phenotypic characterization and strategies for genetic improvement of WAD goats under backyard systems. Open Journal of Animal Sciences, 4(5), 253-262. https://doi.org/10.4236/ojas.2014.45032
Perry, G. A., and Perry, K. C. (2008). Use of vaginal cytology to diagnose estrus in goats. Small Ruminant Research, 74(1–3), 120–125. https://doi.org/10.1016/j.smallrumres.2007.10.005
Raji, U. A., Onubuogu, G. C., and Ibrahim, S. A. (2019). Haematological parameter variations within season, age, sex, parity, and pregnancy in cross bred goats raised in Tiaret, Algeria. Advances in Biology & Earth Sciences, 4(3), 214–221.
Sandabe, U. K., Mustapha, A. R., and Sambo, E. Y. (2004a). Effect of pregnancy on some biochemical parameters in Sahel goats in semi arid zones. Veterinary Research Communications, 28(4), 279–285. https://doi.org/10.1023/B:VERC.0000026655.69812.79
Sandabe, U.K., George, B.D.J. and Njidda, A.A. (2004b). Hematological and serum biochemical indices of Sahel goats during the dry season in the semi-arid zone of Nigeria. Tropical Animal Health and Production, 36(5), 453-461.
Sarangi, B. (2018). Hematological and serum biochemical parameters in different breeds of goats. Indian Journal of Veterinary Sciences and Biotechnology, 14(2), 112-125.
Shittu, O. B., Okwelum, N., Famakinde, S. A., Odeyemi, A. J. and Toviesi, D. P. (2023). Haematological, serum biochemical and hormonal profile in West African Dwarf goats during pregnancy. Agricultura Tropica et Subtropica, 56(1), 50-57. https://doi.org/10.2478/ats-2023-0006
Shittu, O. O., Amole, T. A., Okwelum, N., Odeyemi, J. A., Toviesi, D.P., Ojo, V. I. O., Oluwatosin, B. O., Smith, O. F. and Osinowo, O. A. (2016). Haematological and serum biochemical parameters of West African Dwarf and Kalahari Red goats in the humid tropics. Nigerian Journal of Animal Science, 2, 305-314.
Stahl A. Ultrasonography in Veterinary Medicine: Live Animal Imaging. 2nd ed. Wiley Blackwell; 2015.