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Abstract
Roles of trace elements and minerals in the overall performance, health and wellbeing of horses cannot be underestimated vis a viz deleterious effects on normal functioning of living organisms posed by prolonged exposure to toxic metals. We evaluated the levels of some minerals and trace elements and compared these parameters among different breed, age and gender. Forty-six apparently healthy horses in Ibadan Polo Club, Ibadan, Nigeria were used for this study. Minerals and trace elements analyses were performed using the inductively coupled plasma optical emission spectrometry (ICP-OES) (Varian Vista Pro®, Varian Medical Systems, Inc., California, USA) and inductively coupled plasma mass spectrometry (ICP-MS) (Varian 820 MS®, Varian Medical Systems, Inc., California, USA). Student’s T-test was applied to establish differences in the values of minerals and trace elements in relation to breed, gender, and age. The results revealed statistically significant differences in the levels of Magnesium (Mg), Iron (Fe), and Iodine (I) and in the level of Silicon (Si), Chromium (Cr), and Iridium (Ir) in minerals and trace elements respectively with significantly higher values in Sudanese compared to Cross-bred horses. Mg, Fe, I, Cr, and Ir values were significantly higher in Mares compared to Stallion. Fe, I, Si, Mo and Cr values were significantly higher in young horses than in adult horses. Conclusively, significant variations were observed in the minerals and trace elements values regarding breed, sex, and age of Horses in Nigeria. Regular supplements of minerals lick and trace elements are advised especially for Cross-bred, Stallion, and adult horses.
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- AAdebola, A. O., Adegboyega, S. A. A., Olajuyigbe, A. E. and Oladejo, S. O. (2015). Modelling of land use and land cover change in Ibadan North-West local government area (LGA), Nigeria using satellite imageries and GIS techniques. J. sustain. dev. Afr., 17(7): 138-166. http://www.jsd-africa.com/Jsda/Vol17N.
- Adedokun, R. A. M., Olaogun, S. C. and Alaba, B. A. (2023). Haematological and biochemical profile of apparently healthy horses in Ibadan, Nigeria. Alex. J. Vet. Sci., 77(1): 156-164.doi: 10.5455/ajvs.10877
- Al-Bulushi, S., Shawaf, T. and Al-Hasani, A. (2017). Some hematological and biochemical parameters of different goat breeds in Sultanate of Oman: A preliminary study. Vet World., 10(4): 461.doi: 10.14202/vetworld.2017.461-466.
- Armelin, M. J. A., Ávila, R. L., Piasentin, R. M. and Saiki, M. (2003). Effect of chelated mineral supplementation on the absorption of Cu, Fe, K, Mn and Zn in horse hair. J. Radioanal. Nucl. Che., 258(2): 449-451.doi:10.1023/A:1026278914222
- Balamurugan, B., Ramamoorthy, M., Mandal, R. S. K., Keerthana, J., Gopalakrishnan, G., Kavya, K. and Katiyar, R. (2017). Mineral an important nutrient for efficient reproductive health in dairy cattle. Int. J. Environ. Sci. Technol., 6(1): 694-701.ISSN 2278-3687 (O)
- Boer, J. L., Mulrooney, S. B. and Hausinger, R. P. (2014). Nickel-dependent metalloenzymes. Arch. Biochem. Biophys., 544:142-152.doi: 10.1016/j.abb.2013.09.002
- Coenen, M. (2013). Macro and trace elements in equine nutrition. Equine Appl. and Clin Nutr., 190.doi: 10.1016/B978-0-7020-3422-0-00010-9
- Cygan-Szczegielniak, D. and Stasiak, K. (2022). Concentration of Selected Essential and Toxic Trace Elements in Horse Hair as an Important Tool for the Monitoring of Animal Exposure andHealth. Animals., 12(19),2665. https://doi.org/10.3390/ani12192665
- Cywinska, A., Szarska, E., Kowalska, A., Ostaszewski, P. and Schollenberger, A. (2011). Gender differences in exercise–induced intravascular haemolysis during race training in thoroughbred horses. Res Vet Sci., 90(1):133-137. doi: 10.1016/j.rvsc.2010.05.004.
- Fleming, K. A., Barton, M. H. and Latimer, K. S. (2006). Iron deficiency anaemia in a neonatal foal. J. Vet. Intern. Med., 20(6): 1495-1498.doi: 10.1892/0891-6640(2006)20[1495: idaian]2.0.co;2.
- Forrer, R., Wenker, C. H., Gautschi, K. and Lutz, H. (2001). Concentration of 17 trace elements in serum and whole blood of plains viscachas (Lagostomus maximus) by ICP-MS, their reference ranges, and their relation to cataract. Biol Trace Elem Res., 81(1): 47-62.doi: 10.1385/bter:81:1:47.
- Herdt, T. H. and Hoff, B. (2011). The use of blood analysis to evaluate trace mineral status in ruminant livestock. Veterinary Clinics: Food Anim. Pract., 27(2): 255-283.doi: 10.1016/j.cvfa.2011.02.004.
- Humann-Ziehank, E., Tegtmeyer, P. C., Seelig, B., Roehrig, P., and Ganter, M. (2013). Variation of serum selenium concentrations in German sheep flocks and implications for herd health management consultancy. Acta Vet. Scand., 55(1): 1-8.doi: 10.1186/1751-0147-55-82.
- Jeffery, E. H., Noseworthy, R. and Cherian, M. G. (1989). Age dependent changes in metallothionein and accumulation of cadmium in horses. Comp. Biochem. Physiol. C Pharmacol., 93(2): 327-332.doi: 10.1016/0742-8413(89)90242-9.
- Kalashnikov, V. V., Zajcev, A. M., Atroshchenko, M. M., Miroshnikov, S. A., Zavyalov, O. A., Frolov, A. N. and Skalny, A. V. (2019). Assessment of gender effects and reference values of mane hair trace element content in English thoroughbred horses (North Caucasus, Russia) using ICP-DRC-MS. Biol Trace Elem Res., 191:382-388.doi: 10.1007/s12011-019-1634-9.
- Kalashnikov, V., Zajcev, A., Atroshchenko, M., Miroshnikov, S., Frolov, A., Zav’yalov, O. and Kalashnikova, T. (2018). The content of essential and toxic elements in the hair of the mane of the trotter horses depending on their speed. Environ. Sci. Pollut. Res., 25: 21961-21967.doi: 10.1007/s11356-018-2334-2.
- Kozak, M., Kralova, E., Sviatko, P., Bilek, J. and Bugarsky, A. (2002). Study of the content of heavy metals related to environmental load in urban areas in Slovakia. Bratisl. Lek. Listy., 103(7/8): 231-237.PMID: 12518994
- McGorum, B. C., Wilson, R., Pirie, R. S., Mayhew, I. G., Kaur, H. and Aruoma, O. I. (2003). Systemic concentrations of antioxidants and biomarkers of macromolecular oxidative damage in horses with grass sickness. Equine Vet. J., 35(2): 121-126.doi: 10.2746/042516403776114225.
- Mehri, A. (2020). Trace elements in human nutrition (II)–an update. Int J Prev Med., 11.doi: 10.4103/ijpvm.IJPVM_48_19.
- Olaogun, S. C. and Jeremiah, O. T. (2018). Clinico-haematological features of dermatophilosis in indigenous breeds of cattle in Ibadan, Nigeria. Niger. Vet. J., 39(2): 151-160.doi: 10.4314/nvj.v39i2.7
- Olaogun, S. C. and Onwuzuruike, K. J. (2018). Incidence and biochemical parameters of dermatophilosis in Nigerian cattle breeds from livestock markets, Oyo state, Nigeria. Open Vet. J., 8(1):35-39.doi: 10.4314/ovj.v8i1.6.
- Olaogun, S. C. and Oyetoyinbo, T. E. (2020). Lameness and its associated hematological features among Nigerian breeds of goats in Ibadan, Nigeria. Niger. J. Animal Sci., 22(3): 315-324. (ISSN:1119-4308)
- Olaogun, S. C., Abiola, J. O., Adah, O., Adedokun, R. A. and Ososanya, T. O. (2022). Clinico-Pathological Findings, Diagnosis and Management of an Outbreak of Peste des Petis Ruminants in A Goats’ Flock in Ibadan Oyo State, Nigeria. Alex. J. Vet. Sci., 74(1).doi: 10.5455/ajvs.105484
- Or, M. E., Kayar, A., Maden, M., Sevinç, M., Özcelik, D., Gülyasar, T. and Barutcu, U. B. (2004). Determination of trace elements (Fe, Cu and Zn) in serum and tail hair of healthy horses as a function of nutritional differences in certain months. Niger. Vet. J., 25(1): 9-13.doi: 10.4314/nvj.v25i7.3459
- Schweinzer, V., Iwersen, M., Drillich, M., Wittek, T., Tichy, A., Mueller, A. and Krametter-Froetscher, R. (2017). Macrominerals and trace element supply in sheep and goats in Austria. Vet. Med., 62(2): 62-73.doi: 10.17221/243/2015-VETMED
- Sherwin, C. M., Christiansen, S. B., Duncan, I. J., Erhard, H. W., Lay Jr, D. C., Mench, J. A. and Petherick, J. C. (2003). Guidelines for the ethical use of animals in applied ethology studies. Appli Anim Behav Sci, 81(3), 291-305.
- Stanek, M., Jaworski, Z., Sobotka, W., Lipinski, K. and Olenkowicz, R. (2016). Influence of an organic supplement of copper, zinc and manganese in feed rations on concentrations of these elements in the coat of Polish Konik horses. J. Elem., 21(2).doi: :10.5601/jelem.2015.20.1.914
- Stowe, H. D. and Herdt, T. H. (1992). Clinical assessment of selenium status of livestock. J. Anim. Sci., 70(12): 3928-3933.doi: 10.2527/1992.70123928x.
- Topczewska, J. (2012). Effects of seasons on the concentration of selected trace elements in horse hair. J. Cent. Eur. Agric.doi:10.5513/JCEA01/13.4.1110
- Topczewska, J. and Krupa, W. (2013). Influence of horse breed and housing system on the level of selected elements in horse’s hair. J. Elem., 18(2).doi: 10.5601/jelem.2013.18.2.08
- Weigert, P., Scheck, K., Lemmer, B. and Noreisch, W. (1981). Laboratory diagnosis studies of Haflinger horses and mules (pack-animals of the Federal German Army). 4. Minerals and trace elements in blood and serum. Tierarztl. Prax., 9(3), 403-409.PMID: 7348947
References
AAdebola, A. O., Adegboyega, S. A. A., Olajuyigbe, A. E. and Oladejo, S. O. (2015). Modelling of land use and land cover change in Ibadan North-West local government area (LGA), Nigeria using satellite imageries and GIS techniques. J. sustain. dev. Afr., 17(7): 138-166. http://www.jsd-africa.com/Jsda/Vol17N.
Adedokun, R. A. M., Olaogun, S. C. and Alaba, B. A. (2023). Haematological and biochemical profile of apparently healthy horses in Ibadan, Nigeria. Alex. J. Vet. Sci., 77(1): 156-164.doi: 10.5455/ajvs.10877
Al-Bulushi, S., Shawaf, T. and Al-Hasani, A. (2017). Some hematological and biochemical parameters of different goat breeds in Sultanate of Oman: A preliminary study. Vet World., 10(4): 461.doi: 10.14202/vetworld.2017.461-466.
Armelin, M. J. A., Ávila, R. L., Piasentin, R. M. and Saiki, M. (2003). Effect of chelated mineral supplementation on the absorption of Cu, Fe, K, Mn and Zn in horse hair. J. Radioanal. Nucl. Che., 258(2): 449-451.doi:10.1023/A:1026278914222
Balamurugan, B., Ramamoorthy, M., Mandal, R. S. K., Keerthana, J., Gopalakrishnan, G., Kavya, K. and Katiyar, R. (2017). Mineral an important nutrient for efficient reproductive health in dairy cattle. Int. J. Environ. Sci. Technol., 6(1): 694-701.ISSN 2278-3687 (O)
Boer, J. L., Mulrooney, S. B. and Hausinger, R. P. (2014). Nickel-dependent metalloenzymes. Arch. Biochem. Biophys., 544:142-152.doi: 10.1016/j.abb.2013.09.002
Coenen, M. (2013). Macro and trace elements in equine nutrition. Equine Appl. and Clin Nutr., 190.doi: 10.1016/B978-0-7020-3422-0-00010-9
Cygan-Szczegielniak, D. and Stasiak, K. (2022). Concentration of Selected Essential and Toxic Trace Elements in Horse Hair as an Important Tool for the Monitoring of Animal Exposure andHealth. Animals., 12(19),2665. https://doi.org/10.3390/ani12192665
Cywinska, A., Szarska, E., Kowalska, A., Ostaszewski, P. and Schollenberger, A. (2011). Gender differences in exercise–induced intravascular haemolysis during race training in thoroughbred horses. Res Vet Sci., 90(1):133-137. doi: 10.1016/j.rvsc.2010.05.004.
Fleming, K. A., Barton, M. H. and Latimer, K. S. (2006). Iron deficiency anaemia in a neonatal foal. J. Vet. Intern. Med., 20(6): 1495-1498.doi: 10.1892/0891-6640(2006)20[1495: idaian]2.0.co;2.
Forrer, R., Wenker, C. H., Gautschi, K. and Lutz, H. (2001). Concentration of 17 trace elements in serum and whole blood of plains viscachas (Lagostomus maximus) by ICP-MS, their reference ranges, and their relation to cataract. Biol Trace Elem Res., 81(1): 47-62.doi: 10.1385/bter:81:1:47.
Herdt, T. H. and Hoff, B. (2011). The use of blood analysis to evaluate trace mineral status in ruminant livestock. Veterinary Clinics: Food Anim. Pract., 27(2): 255-283.doi: 10.1016/j.cvfa.2011.02.004.
Humann-Ziehank, E., Tegtmeyer, P. C., Seelig, B., Roehrig, P., and Ganter, M. (2013). Variation of serum selenium concentrations in German sheep flocks and implications for herd health management consultancy. Acta Vet. Scand., 55(1): 1-8.doi: 10.1186/1751-0147-55-82.
Jeffery, E. H., Noseworthy, R. and Cherian, M. G. (1989). Age dependent changes in metallothionein and accumulation of cadmium in horses. Comp. Biochem. Physiol. C Pharmacol., 93(2): 327-332.doi: 10.1016/0742-8413(89)90242-9.
Kalashnikov, V. V., Zajcev, A. M., Atroshchenko, M. M., Miroshnikov, S. A., Zavyalov, O. A., Frolov, A. N. and Skalny, A. V. (2019). Assessment of gender effects and reference values of mane hair trace element content in English thoroughbred horses (North Caucasus, Russia) using ICP-DRC-MS. Biol Trace Elem Res., 191:382-388.doi: 10.1007/s12011-019-1634-9.
Kalashnikov, V., Zajcev, A., Atroshchenko, M., Miroshnikov, S., Frolov, A., Zav’yalov, O. and Kalashnikova, T. (2018). The content of essential and toxic elements in the hair of the mane of the trotter horses depending on their speed. Environ. Sci. Pollut. Res., 25: 21961-21967.doi: 10.1007/s11356-018-2334-2.
Kozak, M., Kralova, E., Sviatko, P., Bilek, J. and Bugarsky, A. (2002). Study of the content of heavy metals related to environmental load in urban areas in Slovakia. Bratisl. Lek. Listy., 103(7/8): 231-237.PMID: 12518994
McGorum, B. C., Wilson, R., Pirie, R. S., Mayhew, I. G., Kaur, H. and Aruoma, O. I. (2003). Systemic concentrations of antioxidants and biomarkers of macromolecular oxidative damage in horses with grass sickness. Equine Vet. J., 35(2): 121-126.doi: 10.2746/042516403776114225.
Mehri, A. (2020). Trace elements in human nutrition (II)–an update. Int J Prev Med., 11.doi: 10.4103/ijpvm.IJPVM_48_19.
Olaogun, S. C. and Jeremiah, O. T. (2018). Clinico-haematological features of dermatophilosis in indigenous breeds of cattle in Ibadan, Nigeria. Niger. Vet. J., 39(2): 151-160.doi: 10.4314/nvj.v39i2.7
Olaogun, S. C. and Onwuzuruike, K. J. (2018). Incidence and biochemical parameters of dermatophilosis in Nigerian cattle breeds from livestock markets, Oyo state, Nigeria. Open Vet. J., 8(1):35-39.doi: 10.4314/ovj.v8i1.6.
Olaogun, S. C. and Oyetoyinbo, T. E. (2020). Lameness and its associated hematological features among Nigerian breeds of goats in Ibadan, Nigeria. Niger. J. Animal Sci., 22(3): 315-324. (ISSN:1119-4308)
Olaogun, S. C., Abiola, J. O., Adah, O., Adedokun, R. A. and Ososanya, T. O. (2022). Clinico-Pathological Findings, Diagnosis and Management of an Outbreak of Peste des Petis Ruminants in A Goats’ Flock in Ibadan Oyo State, Nigeria. Alex. J. Vet. Sci., 74(1).doi: 10.5455/ajvs.105484
Or, M. E., Kayar, A., Maden, M., Sevinç, M., Özcelik, D., Gülyasar, T. and Barutcu, U. B. (2004). Determination of trace elements (Fe, Cu and Zn) in serum and tail hair of healthy horses as a function of nutritional differences in certain months. Niger. Vet. J., 25(1): 9-13.doi: 10.4314/nvj.v25i7.3459
Schweinzer, V., Iwersen, M., Drillich, M., Wittek, T., Tichy, A., Mueller, A. and Krametter-Froetscher, R. (2017). Macrominerals and trace element supply in sheep and goats in Austria. Vet. Med., 62(2): 62-73.doi: 10.17221/243/2015-VETMED
Sherwin, C. M., Christiansen, S. B., Duncan, I. J., Erhard, H. W., Lay Jr, D. C., Mench, J. A. and Petherick, J. C. (2003). Guidelines for the ethical use of animals in applied ethology studies. Appli Anim Behav Sci, 81(3), 291-305.
Stanek, M., Jaworski, Z., Sobotka, W., Lipinski, K. and Olenkowicz, R. (2016). Influence of an organic supplement of copper, zinc and manganese in feed rations on concentrations of these elements in the coat of Polish Konik horses. J. Elem., 21(2).doi: :10.5601/jelem.2015.20.1.914
Stowe, H. D. and Herdt, T. H. (1992). Clinical assessment of selenium status of livestock. J. Anim. Sci., 70(12): 3928-3933.doi: 10.2527/1992.70123928x.
Topczewska, J. (2012). Effects of seasons on the concentration of selected trace elements in horse hair. J. Cent. Eur. Agric.doi:10.5513/JCEA01/13.4.1110
Topczewska, J. and Krupa, W. (2013). Influence of horse breed and housing system on the level of selected elements in horse’s hair. J. Elem., 18(2).doi: 10.5601/jelem.2013.18.2.08
Weigert, P., Scheck, K., Lemmer, B. and Noreisch, W. (1981). Laboratory diagnosis studies of Haflinger horses and mules (pack-animals of the Federal German Army). 4. Minerals and trace elements in blood and serum. Tierarztl. Prax., 9(3), 403-409.PMID: 7348947