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Abstract
Heavy metals bioaccumulation in fish constitute an issue of public health concerns. This study aimed to evaluate the levels of Cadmium (Cd) and Lead (Pb) in the head and muscle tissues of imported frozen fish as well as health risk to man. A total of 30 frozen fish were sampled from different fish markets in Damaturu and Maiduguri in Yobe and Borno States, Nigeria respectively. Heavy metal concentrations were determined by atomic absorption spectrophotometer. The highest mean concentration levels (0.60 ± 0.32 mg/kg and 0.06 ± 0.10 mg/kg) of Pb and Cd were found in the head and the lowest (0.09 ± 0.02 and 0.01 ± 0.00) in the muscle tissues respectively. The mean concentration levels of Pb (0.60 ± 0.32 mg/kg) and Cd (0.06 ± 0.10 mg/kg) in fish markets A and D exceed the maximum allowable limits of 0.50 mg/kg and 0.05 mg/kg respectively. Fish markets had statistically significant (p< 0.05) effect on the concentration levels of Pb among the sampled fish. The assessment of potential health risk; estimated daily intake, the target cancer rate, target hazard quotient and hazard index values obtained were below the threshold, indicating consumption of head and muscle of imported frozen fish sold in the study areas is safe and hazardless. However, because of their non-degradable nature and tendency to accumulate in tissues and organs, we recommended that heavy metals contamination in imported frozen fish should be monitored regularly, and packages should contain concentration labels before releasing it into the market value chain.
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References
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 - Aladaileh, S. H., Khafaga, A. F., Abd El-Hack, M. A., Al-Gabri, N. A., Abukhalil, M. H., Alfwuaires, M. A., Bin-Jumah, M., Alkahtani, S., Abdel-Daim, M. M., Aleya, L. and Abdednour, S. (2020). Spirulina platensis ameliorates the sub chronic toxicities of lead in rabbits via anti-oxidative, anti-inflammatory and immune stimulatory properties. Science of The Total Environment, 701: 134879 DOI: 10.1016/j.scitotenv.2019.134879 
 - American Public Health Association (2015). Quality assurance (1020) quality control. Standard Methods for the Laboratory Examination. DOI: 10.2105/9780875530024ch02 
 - Benard, B. B., Silverleen, D. C. and Chidinma, I. J. (2020). Human health risk assessment of consuming heavy metals in Oyster (Crassostrea virginica) from different markets in Port Harcourt, Rivers State. Earth & Environmental Science Research & Reviews, 3(2): 67-72 URL: https://www.opastpublishers.com/open-access-articles/human-health-risk-assessment-of-consuming-heavy-metals-in-oyster-crassostrea-virginica-from-different-markets-in-port-ha.pdf 
 - Brack, W., Dulio, V., Agerstrand, M., Allan, I., Altenburger, R., Brinkmann,M.,Bunke,D., Burgess, M. R., Cousins, I., Escher, B. I.,Hernández P. J., et al. (2017). Towards the review of the European Union Water Framework Directive: Recommendations for more efficient assessment and management of chemical contamination in European surface water resources. Science of The Total Environment, 576: 720-737 https://doi.org/10.1016/j.scitotenv.2016.10.104 
 - Burger, J., Gochfeld, M., Shukla, T., Jeitner, C., Burke, S., Donio, M., Shukla, S., Snigaroff, R., Snigaroff, D., Stamm, T. and Volz, C. (2007). Heavy metals in Pacific cod (Gadus macrocephalus) from the Aleutians. Location, age, size and risk. Journal of Toxicology and Environmental Health, 70(22): 1897-1911 DOI: 10.1080/15287390701551159 
 - Connell, D. W. and Miller, G. J. (2022). Chemistry and Toxicology of Pollution: Ecological and Human Health. John Wiley & Sons publishers, 2nd Edition. 
 - Dou, J., Bai, Y. and Chen, Q. (2022). Challenges of lead leakage in perovskite solar cells. Materials Chemistry Frontiers, 6(19): 2779-2789 https://doi.org/10.1039/D2QM00632D 
 - Elkady, A. A., Sweet, S. T., Wade, T. L. and Klein, A. G. (2015). Distribution and assessment of heavy metals in the aquatic environment of lake Manzala, Egypt. Ecological Indicators, 58: 445-457 https://doi.org/10.1016/j.ecolind.2015.05.029 
 - Eresanya, E. O., Ajayi, V. O., Daramola, M. T. and Balogun, R. (2018). Temperature extremes over selected stations in Nigeria. Physical Science International Journal, 20: 1-10 DOI: 10.9734/PSIJ/2018/34637 
 - Food and Agriculture Organization; World Health Organization (FAO/WHO) (2011). Evaluation of certain food additives and contaminants (seventy-third report of the joint FAO/WHO expert committee on food additives); WHO technical report series 960 held in Geneva, Switzerland in 2011; World Health Organization: Geneva Switzerland. 
 - Garai, P., Banerjee, P., Mondal, P. and Saha, N. C. (2021). Effect of heavy metals on fishes: toxicity and bioaccumulation. Journal of Clinical Toxicology, 11(18): 001 URL: https://www.longdom.org/open-access/effect-of-heavy-metals-on-fishes-toxicity-and-bioaccumulation-82260.html 
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 - Konduracka, E. (2019). A link between environmental pollution and civilization disorders: a mini review. Reviews on Environmental Health, 34(3): 227-233 DOI: 10.1515/reveh-2018-0083 
 - Latif, M., Zahoor, M., Muhammad, A., Naz, S., Kamran, A. W., Ullah, R., Shah, A. B., Almeer, R. and Sayed, A. (2022). Bioaccumulation of lead in different organs of Ctenopharyngodon Idella (grass fish) and Tor putitora (Mahseer) fish. Brazilian Journal of Biology, 84: 2024 https://doi.org/10.1590/1519-6984.260355 
 - Makedonski, L., Peycheva, K. and Stancheva, M. (2017). Determination of heavy metals in selected black sea fish species. Food Control, 72: 313-318 https://doi.org/10.1016/j.foodcont.2015.08.024 
 - Maulu, S., Hasimuna, O. J., Monde, C. and Mweemba, M. (2020). An assessment of post-harvest fish losses and preservation practices in Siavonga district, Southern Zambia. Fisheries and Aquatic Sciences, 23(1): 1-9 DOI: https://doi.org/10.1186/s41240-020-00170-x 
 - Maulu, S., Nawanzi, K., Abdel-Tawwab, M., and Khalil, H. S. (2021). Fish Nutritional Value as an Approach to Children's Nutrition. Frontiers in Nutrition, 8: 780844 PMID: 34977125 DOI: 10.3389/fnut.2021.780844
 - Mziray, P. and Kimirei, I. A. (2016). Bioaccumulation of heavy metals in marine fishes (Siganus sutor, Lethrinus harak, and Rastrelliger kanagurta) from Dar es Salaam Tanzania. Regional Studies in Marine Science, 7: 72-80 DOI: https://doi.org/10.1016/j.rsma.2016.05.014 
 - Naseri, K., Salmani, F., Zeinali, M. and Zeinali, T. (2021). Health risk assessment of Cd, Cr, Cu, Ni and Pb in the muscle, liver and gizzard of hen’s marketed in East of Iran. Toxicology Report, 8: 53-59 PMID: 33409123 DOI: 10.1016/j.toxrep.2020.12.012 
 - Nathanson, J. A. (2004). Basic environmental technology—water supply, waste management and pollution control. Medical Journal, Armed Forces India, 60(2): 206 DOI: 10.1016/S0377-1237(04)80128-X 
 - Njoga, E. O., Ezenduka, E. V., Ogbodo, C. G., Ogbonna, C. U., Jaja, I. F., Ofomatah, A. C. and Okpala, C. O. R. (2021). Detection, distribution and health risk assessment of toxic heavy metals/metalloids, Arsenic, Cadmium and Lead in Goat carcasses processed for human consumption in South-Eastern Nigeria. Foods, 10: 798 DOI: https://doi.org/10.3390/foods10040798
 - Olaoye O.J., Ojebiyi W.G. and Akinrinola A.O. (2022). Occupational hazards associated with frozen fish marketing in Abeokuta metropolis in Ogun State, Nigeria. Agro-Science Journal of Tropical Agriculture, Food, Environment and Extension, 21 (3): 49-54. DOI: 10.4314/as.v21i3.6 
 - Olayinka-Olagunju, J. O. (2021). Analysis and potential ecological risk assessment of heavy metals in surface water and sediments of Owena River Ondo State. FUTA Journal of Life Sciences, 1(1): 39-53.
 - Perera, P. C. T., Sundarabarathy, T. V., Sivananthawerl, T., Kodithuwakku, S. P. and Edirisinghe, U. (2016). Arsenic and cadmium contamination in water, sediments and fish is a consequence of paddy cultivation: evidence of river pollution in Sri Lanka. Achievements in the Life Sciences, 10(2): 144-160 DOI: https://doi.org/10.1016/j.als.2016.11.002 
 - Peterson, S. A., Van Sickle, J., Herlihy, A. T. and Hughes, R. M. (2007). Mercury concentration in fish from streams and rivers throughout the western United States. Environmental Science and Technology, 41(1): 58-65 DOI: https://doi.org/10.1021/es061070u 
 - Rai, P. K. (2018). Phytoremediation of emerging contaminants in Wetlands. CRC Press, Taylor & Francis, Boca Raton, Florida, USA, PP. 248.
 - Rai, P. K., Lee, S. S., Zhang, M., Tsang, Y. F. and Kim, K. H. (2019). Heavy metals in food crops: health risks, fate, mechanisms, and management. Environment International, 125: 365-385 DOI: https://doi.org/10.1016/j.envint.2019.01.067 
 - Schoeters, G., Hond, E. D., Zuurbier, M., Naginiene, R., Van den Hazel, P., Stilianakis, N., Ronchetti, R. and Koppe, J. G. (2006). Cadmium and children: exposure and health effects. Acta Paediatrica, 95(453): 50-54 DOI: https://doi.org/10.1080/08035320600886232 
 - Tall, A. (2023). Fish trade in Africa: an update. GLOBEFISH: Information and Analysis on Markets and Trade of Fisheries and Aquaculture Products. Food and Agriculture of the United Nations. Available online: https://www.fao.org/in-action/globefish/market-reports/resource-detail/en/c/338418/ (Accessed on 27 September 2023).
 - United State Environmental Protection Agency (USEPA) (2021). Risk-based concentration table. Available online: https://epa-prgs.ornl.gov/cgi-bin/chemicals/csl_search (accessed on 18 January 2021). 
 - Vardhan, K. H., Kumar, P. S. and Panda, R. C. (2019). A review on heavy metal pollution, toxicity and remedial measures: Current trends and future perspectives. Journal of Molecular Liquids, 290: 111197 DOI: https://doi.org/10.1016/j.molliq.2019.111197 
 - Yedjou, C. G., Tchounwou, H. M. and Tchounwou, P. B. (2016). DNA damage, cell cycle arrest, and apoptosis induction caused by lead in human leukemia cells. International Journal of Environmental Research and Public Health, 13(1): 56 PMID: 26703663 DOI: 10.3390/ijerph13010056 
 - Zhang, J., Zhu, L., Li, F., Liu, C., Qiu, Z., Xiao, M. and Cai, Y. (2018). Comparison of toxic metal distribution characteristics and health risk between cultured and wild fish captured from Honghu city, China. International Journal of Environmental Research and Public Health, 15(2): 334 PMID: 29443869 DOI: 10.3390/ijerph15020334 
 
References
Abubakar, A. Uzairu, A., Ekwumemgbo, P. A. and Okunola O. J. (2014). Evaluation of Heavy Metals Concentration in Imported Frozen Fish Trachurus Murphyi Species Sold in Zaria Market, Nigeria. American Journal of Chemistry 4(5): 137-154 URL: http://article.sapub.org/10.5923.j.chemistry.20140405.02.html 
Aladaileh, S. H., Khafaga, A. F., Abd El-Hack, M. A., Al-Gabri, N. A., Abukhalil, M. H., Alfwuaires, M. A., Bin-Jumah, M., Alkahtani, S., Abdel-Daim, M. M., Aleya, L. and Abdednour, S. (2020). Spirulina platensis ameliorates the sub chronic toxicities of lead in rabbits via anti-oxidative, anti-inflammatory and immune stimulatory properties. Science of The Total Environment, 701: 134879 DOI: 10.1016/j.scitotenv.2019.134879 
American Public Health Association (2015). Quality assurance (1020) quality control. Standard Methods for the Laboratory Examination. DOI: 10.2105/9780875530024ch02 
Benard, B. B., Silverleen, D. C. and Chidinma, I. J. (2020). Human health risk assessment of consuming heavy metals in Oyster (Crassostrea virginica) from different markets in Port Harcourt, Rivers State. Earth & Environmental Science Research & Reviews, 3(2): 67-72 URL: https://www.opastpublishers.com/open-access-articles/human-health-risk-assessment-of-consuming-heavy-metals-in-oyster-crassostrea-virginica-from-different-markets-in-port-ha.pdf 
Brack, W., Dulio, V., Agerstrand, M., Allan, I., Altenburger, R., Brinkmann,M.,Bunke,D., Burgess, M. R., Cousins, I., Escher, B. I.,Hernández P. J., et al. (2017). Towards the review of the European Union Water Framework Directive: Recommendations for more efficient assessment and management of chemical contamination in European surface water resources. Science of The Total Environment, 576: 720-737 https://doi.org/10.1016/j.scitotenv.2016.10.104 
Burger, J., Gochfeld, M., Shukla, T., Jeitner, C., Burke, S., Donio, M., Shukla, S., Snigaroff, R., Snigaroff, D., Stamm, T. and Volz, C. (2007). Heavy metals in Pacific cod (Gadus macrocephalus) from the Aleutians. Location, age, size and risk. Journal of Toxicology and Environmental Health, 70(22): 1897-1911 DOI: 10.1080/15287390701551159 
Connell, D. W. and Miller, G. J. (2022). Chemistry and Toxicology of Pollution: Ecological and Human Health. John Wiley & Sons publishers, 2nd Edition. 
Dou, J., Bai, Y. and Chen, Q. (2022). Challenges of lead leakage in perovskite solar cells. Materials Chemistry Frontiers, 6(19): 2779-2789 https://doi.org/10.1039/D2QM00632D 
Elkady, A. A., Sweet, S. T., Wade, T. L. and Klein, A. G. (2015). Distribution and assessment of heavy metals in the aquatic environment of lake Manzala, Egypt. Ecological Indicators, 58: 445-457 https://doi.org/10.1016/j.ecolind.2015.05.029 
Eresanya, E. O., Ajayi, V. O., Daramola, M. T. and Balogun, R. (2018). Temperature extremes over selected stations in Nigeria. Physical Science International Journal, 20: 1-10 DOI: 10.9734/PSIJ/2018/34637 
Food and Agriculture Organization; World Health Organization (FAO/WHO) (2011). Evaluation of certain food additives and contaminants (seventy-third report of the joint FAO/WHO expert committee on food additives); WHO technical report series 960 held in Geneva, Switzerland in 2011; World Health Organization: Geneva Switzerland. 
Garai, P., Banerjee, P., Mondal, P. and Saha, N. C. (2021). Effect of heavy metals on fishes: toxicity and bioaccumulation. Journal of Clinical Toxicology, 11(18): 001 URL: https://www.longdom.org/open-access/effect-of-heavy-metals-on-fishes-toxicity-and-bioaccumulation-82260.html 
Guevara-García, A. A., Juárez, K. and Herrera-Estrella, L. R. (2017). Heavy metal adaptation. eLS Encyclopedia of Life Sciences. John Wiley & Sons, Ltd., Chichester, Pp. 1-9.
Konduracka, E. (2019). A link between environmental pollution and civilization disorders: a mini review. Reviews on Environmental Health, 34(3): 227-233 DOI: 10.1515/reveh-2018-0083 
Latif, M., Zahoor, M., Muhammad, A., Naz, S., Kamran, A. W., Ullah, R., Shah, A. B., Almeer, R. and Sayed, A. (2022). Bioaccumulation of lead in different organs of Ctenopharyngodon Idella (grass fish) and Tor putitora (Mahseer) fish. Brazilian Journal of Biology, 84: 2024 https://doi.org/10.1590/1519-6984.260355 
Makedonski, L., Peycheva, K. and Stancheva, M. (2017). Determination of heavy metals in selected black sea fish species. Food Control, 72: 313-318 https://doi.org/10.1016/j.foodcont.2015.08.024 
Maulu, S., Hasimuna, O. J., Monde, C. and Mweemba, M. (2020). An assessment of post-harvest fish losses and preservation practices in Siavonga district, Southern Zambia. Fisheries and Aquatic Sciences, 23(1): 1-9 DOI: https://doi.org/10.1186/s41240-020-00170-x 
Maulu, S., Nawanzi, K., Abdel-Tawwab, M., and Khalil, H. S. (2021). Fish Nutritional Value as an Approach to Children's Nutrition. Frontiers in Nutrition, 8: 780844 PMID: 34977125 DOI: 10.3389/fnut.2021.780844
Mziray, P. and Kimirei, I. A. (2016). Bioaccumulation of heavy metals in marine fishes (Siganus sutor, Lethrinus harak, and Rastrelliger kanagurta) from Dar es Salaam Tanzania. Regional Studies in Marine Science, 7: 72-80 DOI: https://doi.org/10.1016/j.rsma.2016.05.014 
Naseri, K., Salmani, F., Zeinali, M. and Zeinali, T. (2021). Health risk assessment of Cd, Cr, Cu, Ni and Pb in the muscle, liver and gizzard of hen’s marketed in East of Iran. Toxicology Report, 8: 53-59 PMID: 33409123 DOI: 10.1016/j.toxrep.2020.12.012 
Nathanson, J. A. (2004). Basic environmental technology—water supply, waste management and pollution control. Medical Journal, Armed Forces India, 60(2): 206 DOI: 10.1016/S0377-1237(04)80128-X 
Njoga, E. O., Ezenduka, E. V., Ogbodo, C. G., Ogbonna, C. U., Jaja, I. F., Ofomatah, A. C. and Okpala, C. O. R. (2021). Detection, distribution and health risk assessment of toxic heavy metals/metalloids, Arsenic, Cadmium and Lead in Goat carcasses processed for human consumption in South-Eastern Nigeria. Foods, 10: 798 DOI: https://doi.org/10.3390/foods10040798
Olaoye O.J., Ojebiyi W.G. and Akinrinola A.O. (2022). Occupational hazards associated with frozen fish marketing in Abeokuta metropolis in Ogun State, Nigeria. Agro-Science Journal of Tropical Agriculture, Food, Environment and Extension, 21 (3): 49-54. DOI: 10.4314/as.v21i3.6 
Olayinka-Olagunju, J. O. (2021). Analysis and potential ecological risk assessment of heavy metals in surface water and sediments of Owena River Ondo State. FUTA Journal of Life Sciences, 1(1): 39-53.
Perera, P. C. T., Sundarabarathy, T. V., Sivananthawerl, T., Kodithuwakku, S. P. and Edirisinghe, U. (2016). Arsenic and cadmium contamination in water, sediments and fish is a consequence of paddy cultivation: evidence of river pollution in Sri Lanka. Achievements in the Life Sciences, 10(2): 144-160 DOI: https://doi.org/10.1016/j.als.2016.11.002 
Peterson, S. A., Van Sickle, J., Herlihy, A. T. and Hughes, R. M. (2007). Mercury concentration in fish from streams and rivers throughout the western United States. Environmental Science and Technology, 41(1): 58-65 DOI: https://doi.org/10.1021/es061070u 
Rai, P. K. (2018). Phytoremediation of emerging contaminants in Wetlands. CRC Press, Taylor & Francis, Boca Raton, Florida, USA, PP. 248.
Rai, P. K., Lee, S. S., Zhang, M., Tsang, Y. F. and Kim, K. H. (2019). Heavy metals in food crops: health risks, fate, mechanisms, and management. Environment International, 125: 365-385 DOI: https://doi.org/10.1016/j.envint.2019.01.067 
Schoeters, G., Hond, E. D., Zuurbier, M., Naginiene, R., Van den Hazel, P., Stilianakis, N., Ronchetti, R. and Koppe, J. G. (2006). Cadmium and children: exposure and health effects. Acta Paediatrica, 95(453): 50-54 DOI: https://doi.org/10.1080/08035320600886232 
Tall, A. (2023). Fish trade in Africa: an update. GLOBEFISH: Information and Analysis on Markets and Trade of Fisheries and Aquaculture Products. Food and Agriculture of the United Nations. Available online: https://www.fao.org/in-action/globefish/market-reports/resource-detail/en/c/338418/ (Accessed on 27 September 2023).
United State Environmental Protection Agency (USEPA) (2021). Risk-based concentration table. Available online: https://epa-prgs.ornl.gov/cgi-bin/chemicals/csl_search (accessed on 18 January 2021). 
Vardhan, K. H., Kumar, P. S. and Panda, R. C. (2019). A review on heavy metal pollution, toxicity and remedial measures: Current trends and future perspectives. Journal of Molecular Liquids, 290: 111197 DOI: https://doi.org/10.1016/j.molliq.2019.111197 
Yedjou, C. G., Tchounwou, H. M. and Tchounwou, P. B. (2016). DNA damage, cell cycle arrest, and apoptosis induction caused by lead in human leukemia cells. International Journal of Environmental Research and Public Health, 13(1): 56 PMID: 26703663 DOI: 10.3390/ijerph13010056 
Zhang, J., Zhu, L., Li, F., Liu, C., Qiu, Z., Xiao, M. and Cai, Y. (2018). Comparison of toxic metal distribution characteristics and health risk between cultured and wild fish captured from Honghu city, China. International Journal of Environmental Research and Public Health, 15(2): 334 PMID: 29443869 DOI: 10.3390/ijerph15020334 
