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Oct 15, 2021
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Mar 16, 2022
Published
Mar 31, 2022
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Abstract

Smoked dried catfish is a major source of protein in resource-poor countries. Unhygienic processing and handling of fish are a source of foodborne pathogens such as Staphylococcus aureus. This study aimed to determine the bacteriological quality of ready to eat smoked dried catfish and the antimicrobial susceptibility pattern of S. aureus from catfish in Kaduna, Metropolis, Nigeria. Three hundred smoked catfish specimens were collected from 4 markets (Central, Mando, Kawo and Tudun wada)and processed. The smoked dried catfish were subjected to total aerobic and coliform count, isolation and antimicrobial susceptibility testing of S. aureus. The results were analysed with descriptive statistics and presented in tables. The overall mean total aerobic and coliform counts were 3.01 × 106 CFU/g and 1.41 × 106 CFU/g, respectively. The Central market had the highest aerobic count (5.63 × 106 CFU/g), and Mando market had the highest coliform count (2.7 × 106 CFU/g). The overall isolation rate of S. aureus from smoked catfish was 276 (92.0%). Mando market had the highest isolation of S. aureus 74 (98.7%), while Kawo and Tudun Wada markets had 67 (89.3%). Of the 276 S. aureus isolates, 225 (75.0%) were identified as coagulase positive. The isolate showed the lowest resistance to Ciprofloxacin 21 (9.3%), Gentamycin 21 (9.3%) and Chloramphenicol 39 (17.3%). Resistance to Ampicillin and Penicillin was 100%. Multi drug resistantS. aureus contamination of ready to eat smoked dried catfish pose a severe public health hazard to the consumers.

Keywords

Antimicrobial Susceptibility testing; Fish, Foodborne pathogens; Microbiology; Staphylococcus aureus; MDR-Staphylococcus ‎aureus; Resistance

Article Details

How to Cite
Bala, M., Ejeh, E. F., & Saidu, A. S. (2022). The Bacteriological Quality and Multi-drug Resistant Staphylococcus aureus ‎Isolated from Smoked Dried Catfish (Clarias gariepinus) in Kaduna ‎Metropolis, Nigeria‎. Sahel Journal of Veterinary Sciences, 19(1), 15-21. https://doi.org/10.54058/saheljvs.v19i1.281

References

  1. Ababouch, A. (2005) Assessment of foodborne bacterial ‎pathogens that causes product rejection and ‎detention in international seafood trade. Journal ‎of Economic Cooperation and Development, 7 51-‎‎86.‎
  2. Adullahi, I. O., Umoh, V.J., Ameh, J. B., and Galadima, M. ‎‎(2005). Some hazards associated with the ‎production of popular roasted meat (tsire) in Zaria, ‎Nigeria. Journal of Food Control, 12: 242- 351.‎
  3. Adzitey, F., Hudu, N., and Ali, G. R. R. (2012). Prevalence ‎and antibiotics resistance of Campylobacter, ‎Salmonella, and Listeria monocytogenes in ducks- ‎A review of Foodborne Pathogen Disease, 9: 498- ‎‎505.‎
  4. ‎ Agbebi, F. O., and Fagbote, T. A. (2012). The role of ‎middlemen in fish marketing in Igbokoda fish ‎market, Ondo-state, south western Nigeria. ‎International Journal of Development and ‎Sustainability, 1 (3), 880-888.‎
  5. Agu, K. C., Ukponmwa, I. O., Orji, M. U., Awah, N. S., ‎Anaso, C. I,. and Udemezue, O.I. (2013). ‎Prevalence of pathogenic bacteria in smoked fish ‎sold in major retailed ‎market in Benin. ‎International Journal of Applied Science and ‎Engineering, 1(1): 1- 4‎
  6. Austin, B., and Austin, D. (1989). General Introduction. In ‎Methods for Microbiological Examination of fish ‎and shellfish, Austin, B. and Austin, D. A. (Ed) ‎Ellis Horwood Limited, England, p19-24.‎
  7. Becker, K., Skov, R. L., and von Eiff, C. (2015). ‎Staphylococcus, Micrococcus, and other ‎catalase‐positive cocci. Manual of Clinical ‎Microbiology, 354-382.‎
  8. Bauer, A. W., Kirby, W.M., Sherris, J. C., and Turck, M. ‎‎(1966). Antibiotic‎ susceptibility ‎ testing by a ‎standardised single disc method. Antimicrobial ‎Journal of Clinical Pathology, 45: 493-496.‎
  9. Bintsis, T. (2017). Foodborne pathogens. AIMS ‎microbiology, 3(3), 529 563. https://doi.org/10.39‎‎34/microbiol.2017.3.529‎
  10. Cowan, S. T and Steel, K. J. (1993). Cowan and Steels, ‎manual for identification of Medical bacteria. ‎Cambridge University Press.3rd ed. Barrow GI and ‎Feltham RKA. P 199-241.‎
  11. Das, A. P, Kumar, P. S., Swain, S. (2014.) Recent advances ‎in biosensor based endotoxin detection. Biosens ‎Bioelectron 51:62–75‎
  12. Faisal, S. D., Ahmed, A. F.,(2018). Bacteriological Quality ‎Assessment of Milk in College of Veterinary ‎Medicine (CVM) Dairy Farm and Kalamino Dairy ‎Farm in Mekelle, Tigray, Ethiopian Dairy and ‎Veterinary Science Journal., 8(2): 555734. DOI: ‎‎10.19080/JDVS.2018.08.555734.‎
  13. Fox, M., Mitchell, M., Dean, M. (2018). The seafood supply ‎chain from a fraudulent perspective. Food ‎Secience. 10, 939–963 (2018). ‎https://doi.org/10.1007/s12571-018-0826-z
  14. Haifang, Z., Yi, Z., Huasheng, G., Ping, X., Min, W., Aiqing, ‎L., Minhui, M., Xiaofang, X., Yimai, D., Huiqin, ‎Z., , and Hong, D. (2016) Identification and ‎Characterisation of Staphylococcus aureus Strains ‎with an Incomplete Hemolytic Phenotype. ‎Frontier. Cells and Infection and Microbiology. https://doi.org/10.3389/fcimb.20‎‎16.00146‎
  15. Humphries, R. M., Ambler, J., Mitchell, S. L., Castanheira, ‎M., Dingle, T., Hindler, J. A. and Sei, K. (2018). ‎CLSI methods development and standardisation ‎working group best practices for evaluation of ‎antimicrobial susceptibility tests. Journal of ‎Clinical Microbiology, 56(4), e01934-‎
  16. International Commission on Microbiological ‎Specifications for Foods (ICMSF). ‎‎(1996). Microorganisms in foods 5: ‎Characteristics of microbial pathogens (Vol. 5). ‎Springer Science and Business Media. London. ‎‎1996. 1st ed.. 508 p..‎
  17. Ikutegbe, V,. and Sikolo, F. (2014). Mirobial and ‎biochemical spoilage of smoked dried fish sold in ‎West African open markets. Food Chemistry, 161:‎‎332-336.‎
  18. Imre, K., Herman, V., and Morar, A. (2020). Scientific ‎Achievements in the Study of the Occurrence and ‎Antimicrobial Susceptibility Profile of Major ‎Foodborne Pathogenic Bacteria in Foods and Food ‎Processing Environments in Romania: Review of ‎the Last Decade. BioMed Research ‎International, https://doi.org/10.1‎‎155/2020/5134764‎
  19. Jager, J., Putnick, D. L. and Bornstein, M. H. (2017). II. ‎More than just convenient: The scientific merits of ‎homogeneous convenience samples. Monographs ‎of the Society for Research in Child ‎Development, 82(2), 13-30.‎
  20. Khatib, R. M., Naber, N., Shellum, L., Ashton, K., Knowles, ‎V., Giardina, B., and Wilson, M. (1994). A ‎common source outbreak of gastroenteritis in a ‎teaching hospital. Infection Control and Hospital ‎Epidemiology, 15: 534-535‎
  21. Kwaga, J. K. P and Adesiyun, A. A. (1984). Antibiograms of ‎Staphylococcus aureus isolates from some Ready-‎To-Eat products. Journal of Food Protection, 47: ‎‎865-867.‎
  22. Lagier, J. C., Edouard, S., Pagnier, I., Mediannikov, O., ‎Drancourt, M., Raoult, D. (2015) Current and past ‎strategies for bacterial culture in clinical ‎microbiology. Clinical Microbiology ‎Review.;28(1):208-36‎
  23. Lee, J. H. (2003). Methicillin (Oxacillin)-resistance ‎Staphylococcus aureas strains isolated from a ‎major food animals and their potentials ‎transmission to humans. Applied and ‎Enviromental Microbiology, 69 (11):6489-649.‎
  24. Mellou, K., Kyritsi, M., Chrysostomou, A., Sideroglou, T., ‎Georgakopoulou, T., and Hadjichristodoulou, C. ‎‎(2019). Clostridium perfringens Foodborne ‎Outbreak during an Athletic Event in Northern ‎Greece, June 2019. International journal of ‎Environmental Research and Public ‎Health, 16(20), 3967. ‎https://doi.org/10.3390/ijerph16203967‎
  25. Michael, T. S., Brian, VL, Stefan, S. and Jeffrey, L. W. ‎‎(2018) Applying definitions for multidrug ‎resistance, extensive drug resistance and pandrug ‎resistance to clinically significant livestock and ‎companion animal bacterial pathogens, Journal of ‎Antimicrobial Chemotherapy, 73 (6): 1460–‎‎1463, https://doi.org/10.1093/jac/dky043‎
  26. Muhammad, N., Mpyet, C., Adamu, M. D., William, A., ‎Umar, M. M., Goyol, M., Muazu, H., Onyebuchi, ‎U., Isiyaku, S., Flueckiger, R. M., Chu, B. K., Willis, ‎R., Pavluck, A. L., Alhassan, A., Olobio, N., ‎Gordon, B. A. and Solomon, A. W. (2016). ‎Mapping Trachoma in Kaduna State, Nigeria: ‎Results of 23 Local Government Area-Level, ‎Population-Based ‎Prevalence Surveys. Ophthalmic Epidemiology, 23‎‎(sup1),46–54. ‎https://doi.org/10.1080/09286586.2016.1250918‎
  27. Novothy, L. L, Drorska, A., Lorencora, V. B and pavlik, L. ‎‎(2004). Fish: A potential source of bacterial ‎pathogens for human beings. Veterinary Medicine ‎Czeach, 49:343-358. ‎
  28. Olonitola, O. S., Olayinka, B. O., Salawu, M. J., and ‎Yakubu, S. E. (2007). Nasal carriage of methicillin-‎resistant Staphylococcus aureus with reduced ‎vancomycin susceptibility (MRSA-RVS) by ‎healthy adults in Zaria, Nigeria. Journal of ‎Tropical Microbiology and Biotechnology, 3: 19-‎‎22.‎
  29. Olonitola, O. S., Oniye, S. J., Abdullahi, I. O and Okunade, ‎E. A. (2006). Bacteriological quality of some dried ‎fish species in Sabon-Gari market, Zaria, Nigeria. ‎Best Journal, 3(2):87-89.‎
  30. Omojowo, F. S and Ihauhi, J. A. (2006). Microbial quality ‎and safety of smoked ‎fish from Kainji lake ‎area. Africa scientist volume 7, (4):177-181.‎
  31. Pandey, P. K., Kass, P. H, Soupir, M. L, Biswas, S., and ‎Singh V. P. (2014). Contamination of water ‎resources by pathogenic bacteria. AMB Express, 4, ‎‎51. https://doi.org/10.1186/s13568-014-0051-x
  32. Pigott, G., and Tuckker, B. (1990). Seafood effects of ‎technology on nutrient, Marcel Deckker, inc. ‎New York .p 155-170.‎
  33. Salgado-Pabón, W. and Schlievert, P. (2014) Models ‎matter: the search for an ‎effective Staphylococcus ‎aureus vaccine. Nature Review of Microbiology 12, 585–591 ‎https://doi.org/10.1038/nrmicro3308‎
  34. Sanjee, S. A. and Karim, M. (2016). Microbiological quality ‎assessment of frozen fish and fish processing ‎materials from Bangladesh. International ‎Journal of Food Science, vol. 2016, Article ‎ID 8605689, 6 pages, https://doi.org/10.1155/‎‎2016/8605689‎
  35. Schmidt, A.S., Bruun, M. S., Dalsgaard, Pedersen K., and ‎Larsen, J. (2000). Occurrence of antimicrobial ‎resistance in fish-pathogenic and ‎environmental bacteria associated with ‎Danish rainbow trout farm. Applied ‎Environmental Microbiology, 66: 4908-4915.‎
  36. Sokari, T. (1991). Distribution of enterotoxigenic ‎Staphylococcus aureus in ready-to-eat foods ‎in ‎ Eastern Nigeria. International Journal of ‎Food Microbiology, 12: 275-279.‎
  37. Umoh, V. J., Adesiyun, A. A and Gomwalk, N. E. (1990). ‎Antibiogram of Staphylococcal strain isolated ‎from milk and milk products. Journal of ‎Veterinary Medicine, 37:701-706.‎
  38. WHO (2020a) Antimicrobial Resistance: Key Facts. ‎who.int/news room/fact sheets/detail/antimicrobial-resistance. Accessed 8/18/2020 at 6:44 A.M
  39. WHO (2020b) Food Safety: Key Facts. ‎https://www.who.int/news-room/fact-‎sheets/detail/food-safety. Accessed 8/18/2020‎
  40. Whong, C. M. Z, Oniye, S. J. and Otse, G. (2003). ‎Bacteriological quality of smoked –dried ‎Clariasgariepinus (Teugels) and Labeo ‎senegalensis (Cuvier and Valenciennes). ‎Journal of Tropical Biosciences, 3:99-102.‎
  41. Yuasa A, Murata T, Imai K, Yamamoto Y, and Fujimoto Y ‎‎(2019). Treatment procedures and associated ‎medical costs of methicillin resistant Staphylococcus aureus infection in Japan: A retrospective ‎analysis using a database of Japanese ‎employment-based health insurance. SAGE open ‎medicine, 7, ‎‎2050312119871181.https://doi.org/10.1177/2050‎‎312119871181‎