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Jul 18, 2020
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May 31, 2021
Published
Jun 30, 2021
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Abstract

Tuberculosis (TB) is recognized as the most important threat to human and animal health causing morbidity, mortality, and economic losses. A study was conducted to assess the epidemiology and zoonotic implications of TB in two states of northeastern Nigeria, using abattoir surveillance, culture, and molecular typing methods. The human sputum of in-patients from selected hospitals within the study areas was also collected, cultured, and subjected to molecular typing. Out of the 250 human sputa collected, 74 (29.6%) were positive for culture and 40(16%) were positive on Bioline® analysis. Also, of the 200 tissues collected, 62(32.5%) were AFB positive and 25(12.5%) were positive on culture and 18(9.0%) were positive on Bioline® analysis. All positive isolates on Bioline® analyses were further subjected to Genotype® MBTC analysis. A total of (n = 58) isolates; sputum (n=40) and tissue (n=18), identified as MBTC were further subjected to Genotype MBTC® Hain assay PCR analysis. in order to differentiate them into their various species. Of the MBTC isolates from the tissues, 8(4.0%) were found to be Mycobacterium bovis, 5(2.5%) were Mycobacterium tuberculosis and the remaining 5(2.5%) were unidentified using the standard banding patterns. The 40 MBTC from sputum showed 30 (75%) as Mycobacterium tuberculosis 6(15%) were Mycobacterium bovis, 3(7.5%) were Mycobacterium africanum and 1(0.4%) was unidentified using the standard banding patterns. In conclusion, the study revealed the public health importance of bTB in the study area. There is also the need for public health awareness programs on the zoonotic nature of bTB among the abattoir workers and the herdsmen.

Keywords

Bauchi; Cattle; Gombe; Humans; Molecular; Tuberculosis ‎

Article Details

Author Biographies

S. Ibrahim, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, P.M.B., 1013, Zaria, Kaduna State, Nigeria.

Senior Lecturer

1Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria.

S. Danbirni, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, P.M.B., 1013, P.M.B., 1013, Zaria, Kaduna State, Nigeria.

Senior Lecturer

1Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, P.M.B., 1013, Zaria, Kaduna State, Nigeria.

A. S. Saidu, Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria

Lecturer I +2348039359544

Department of Veterinary Public Health & Preventive Medicine,

Faculty of Veterinary Medicine, University of Maiduguri, Maiduguri, Borno State. Nigeria.

Specialty: Public Health & Epidemiology, with special interest in Infectious Diseases and Zoonoses 

B. A. Usman, Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, P.M.B., 1013, Zaria, Kaduna State, Nigeria.

Senior Lecturer,

1Department of Veterinary Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University Zaria, Kaduna State, Nigeria.

B. Y. Kaltungo, Veterinary Teaching Hospital, Ahmadu Bello University, P.M.B., 1013, Zaria, Kaduna State, Nigeria.

Senior Lecturer,

Veterinary Teaching Hospital, Ahmadu Bello University, P.M.B., Zaria, Kaduna State, Nigeria.

F. U. Mohammed, Veterinary Teaching Hospital, Ahmadu Bello University, P.M.B., 1013, Zaria, Kaduna State, Nigeria.

Senior Lecturer,

Veterinary Teaching Hospital, Ahmadu Bello University, P.M.B., Zaria, Kaduna State, Nigeria.

How to Cite
Ibrahim, S., Danbirni, S., Saidu, A. S., Usman, B. A., Kaltungo, B. Y., & Mohammed, F. U. (2021). Molecular Epidemiology of Tuberculosis in Cattle and Human Patients in ‎Bauchi and Gombe States, Northeastern Nigeria: . Sahel Journal of Veterinary Sciences, 18(2), 23-32. https://doi.org/10.54058/saheljvs.v18i2.179

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