Main Article Content


Acalypha wilkesiana has been used for various medicinal purposes such as in the treatment of hypertension, fungal and bacterial infections. In this study, the effects of prolonged oral administration of the aqueous leaf extract on some biochemical profiles of mice were evaluated. A total of 80 mice were allocated to 4 groups (A, B, C and D) comprising of 20 mice each. Mice in group A (control) were given (1 mL) of distilled water orally while 1 mL of the extract was given to the mice in groups B, C and D daily at the concentrations of 800, 1000 and 1200 mg/kg, respectively. This was continued for three weeks and at the end of each week, 4 mice from each group were humanely sacrificed and sera from blood samples collected were used for determination of activities of biochemical parameters. The results showed that administration of the extract resulted in significantly (p < 0.05)
lower concentrations of total protein and cholesterol in the treatment groups at the 3rd week of treatment when compared to control values. Glucose level was significantly (p > 0.05) higher in the extract-treated groups by the 3rd week of treatment, while activities of AST, ALT and ALP were not significantly (p > 0.05) altered by the extract. There were no significant (p > 0.05) differences between the levels of calcium, total and conjugated bilirubin in treatment and control groups, while sodium concentration was significantly (p < 0.05) higher at day 14 after withdrawal of treatment, compared to the control. This study suggests that the extract of Acalypha wilkesiana has a hyperglycaemic effect.


Acalyphya wilkesiana, Aqueous Leaf Extract; Serum Biochemistry; Liver

Article Details

Author Biographies

H. A. Madziga, University of Maiduguri

Department of Veterinary Physiology and Biochemistry.


M. Chiroma

Department of Veterinary Physiology and Biochemistry.


S. Sanni

Department of Veterinary Pharmacology and Toxicology University of Abuja

U. K. Sandabe

Department of Veterinary Physiology and Biochemistry.

How to Cite
Madziga, H. A., Chiroma, M., Sanni, S., Sandabe, U. K., & Sodipo, O. A. (2020). Effects of Aqueous Leaf Extract of Acalypha wilkesiana on some Serum ‎Biochemical Profiles of Mice in Sub-Acute Toxicity Studies. Sahel Journal of Veterinary Sciences, 17(3), 1-5. Retrieved from


  1. Adesina, S.K., O. Idowu, A.O. Ogundaini, H. Oladimeji, T.A. ‎Olugbade, G.O. Onawunmi and M. Pais, (2000). ‎Antimicrobial constituents of the leaves of ‎Acalypha wilkesiana and Acalypha hispida. ‎Phytotheraphy Res. 14: 371-372‎
  2. Akinde B.E. (1986). Phytochemical and Microbiological ‎Evaluation of the oils from the leaves of Acalypha ‎wilkesiana. In: (A Sofowara,ed) The State of ‎Medicinal Plant Research in Nigeria University of ‎Ibadan Press, Nigeria, Pp. 362-363.‎
  3. Alade, P.I., and O.N. Irobi, (1993). Antimicrobial activities ‎of crude leaf extracts of Acalypha ‎ wilkesiana. J. ‎Ethropharmocol. 39: 171-174‎
  4. Allain, C. C., Poon, L. S. and Chan, C. S. G (1974). ‎Enzymatic determination of total serum ‎cholesterol. Clin. Chem. 20: 470.‎
  5. Anon, (2010).; ‎ai.172623908;ct,1/o1/.7 Acessed date 20/8/2010.‎
  6. Astoor, A. M. and Kings, E. J. (1954). Estimation of blood ‎glucose. J. Biochem. 56- 59.‎
  7. ‎ Babson, L. A., Greeley, L. J., Coleman, C. M. and Phillips, G. ‎D. (1966). In vitro determination of alkaline ‎phosphatase in serum or plasma. Clin. Chem. 12: ‎‎482-490‎
  8. Coles, E.H. (1974). Veterinary Clinical Pathology 2nd Edition ‎W.B. Saunder Company, Philadelphia, London, ‎Toronto Pp. 190-271 ‎
  9. CIOMS and ICLAS (2012). Council for International ‎Organisations of Medical Sciences and the ‎International Council for Laboratory Animal ‎Science. International Guiding Principles for ‎Biomedical Research Involving Animal, December ‎‎2012.‎
  10. Edwards, C. R. W., Bouchier, I. A. D, Haslet, C. and Chilvers‎, E.R. (1995). Davidson’s Principles and Practice of ‎Medicine, 17th edn. Churchill living stone, Pp-490.‎
  11. Gotto, A.M. (1997). Cholesterol management in theory and ‎practice Circulation, 96: 4424-4425‎
  12. Gueroui, M. and Kechrid, Z.(2016). Evaluation of some ‎biochemical parameters and brain oxidative stress ‎in experimental rats exposed chronically to silver ‎nitrate and the protective role of vitamin E and ‎selenium. Toxicol. Res. 32(4) 301-309‎
  13. Ikewuchi, J, C. A.,Yovwe, A.U. E. and Osas, O. S. (2008). ‎Effects fo Acalypha wilkesiana Muell Arg on ‎Plasma sodium and potassium concentration of ‎normal rabbits. Pak. J. Nutri.7 (1) 130-132‎
  14. Jendrassik, L. and Grof, P. (1938). Calorimetric method for ‎the determination of total bilirubin, Biochem 2: ‎‎297:8‎
  15. Mayne; P. D. (1998). Clinical Chemistry. Diagnosis and ‎Treatment 6th ed. London: Arnold ‎International;119-204. ‎
  16. Ogundaini, A.O. (2005) From Green into Medicine: Taking a ‎lead from Nature. An inaugural lecture series 176, ‎delivered at Oduduwa Hall, M. Obafemi Awololwo ‎University, Ile Ife, Nigeria p. 12-15‎
  17. Oliver, B. (1959) Medicinal Plants in Nigeria, Nigerian ‎College of Arts, Science and Technology, Ibadan ‎p. 4‎
  18. Riley, H. P. (1963). Families of Flowering Plants of Southern ‎Africa, University of Kenturky Press, USA, Pp. 73.‎
  19. Seebaluck, R., Gurib-Fakim, A., & Mahomoodally, F. ‎‎(2015). Medicinal plants from the genus Acalypha ‎‎(Euphorbiaceae)-A review of their ‎ethnopharmacology and phytochemistry. In J. ‎Ethnopharmacol. 15: 159; 137-157.‎
  20. Sodipo,O.A., Abdulrahman F.I., Sandabe U.K and Akinniyi ‎F.I (2009). Effect of Solanum macrocarpum Linn. ‎on biochemical liver function in diet-induced ‎hypercholesterolaemic rats. Nig. Vet. J. 30 (1): 1- 8.‎