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Abstract

This study was carried out to describe the morphophysiology of the epididymis of the adult African sideneck turtle using histological, microstereological and ultrastructural methods. The epididymal duct lies within a relatively thin sheath of connective tissue, and is lined by pseudostratified columnar epithelium. Unlike luminal diameter and stereocilial height, epithelial height, as well as the population of principal cells, decreased from the proximal to posterior segment. The clear cells of the turtle epididymis are limited to the posterior segment of the duct. Basal and apical cells as well as intra-epithelial lymphocytes are all distributed across the three segments of the epididymis while macrophage-like cells are absent throughout the length of the duct epithelium. The structure of the African sideneck turtle epididymis demonstrates, as in most mammals and few reptiles studied to date, obvious regional differentiation of the duct epithelium with evidences of secretory and endocytotic abilities as demonstrated by the contents of highly developed endoplasmic reticulum and secretory blebs in the principal and basal cells as well as clear cells, believed to be concerned with endocytosis. The outcome of the study is expected to be useful in the comparative structural and functional anatomy of turtle epididymis.

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Morphophysiology of the Epididymis of the African Sideneck Turtle (Pelusios ‎castaneus): Histological, Microstereological and Ultrastructural Approach. (2020). Sahel Journal of Veterinary Sciences, 17(1), 17-25. https://doi.org/10.54058/saheljvs.v17i1.57

How to Cite

Morphophysiology of the Epididymis of the African Sideneck Turtle (Pelusios ‎castaneus): Histological, Microstereological and Ultrastructural Approach. (2020). Sahel Journal of Veterinary Sciences, 17(1), 17-25. https://doi.org/10.54058/saheljvs.v17i1.57

References

  1. Adamali, H.I. and Hermo, L. (1996). Apical and narrow cells ‎are distinct cell types differing in their structure, ‎distribution, and functions in the adult rat epididymis. ‎J.Androl., 17(3):208-222 ‎
  2. Aire, T.A. (2007). Anatomy of the testis and male ‎reproductive tract. In: Jamieson BGM, Ed. Reproductive ‎biology and phylogeny of birds. NH: Science Publishers, ‎Enfield, pp 37-113 ‎
  3. Akbarsha, M.A., Tamilarasan, V. andKadalmani, B. (2007). ‎Light and electron microscopic observations of ‎fabrication, release, and fate of biphasic secretion ‎granules produced by epididymal epithelial principal ‎cells of the fan-throated lizard ‎Sitanaponticerianacuvier. J.Morphol. 267:713–729 ‎
  4. Arrighi, S., Romanello, M.G. andDomeneghini, C. (1993). ‎Ultrastructure of epidi¬dymal epithelium in ‎Equuscaballus. Ann. Anat., 175:1-9 ‎
  5. Broadley, D.G. and Boycott, R.C. (2009). ‎Pelusiossinuatus(Smith 1838) –Serrated Hinged ‎Terrapin. Conservation Biology of Freshwater Turtles ‎and Tortoises. Chel. Res.Monogr., 5: 036.1-036.5 ‎
  6. Cabral, S.R.P., Zieri, R., Franco-Belussi, L., Santos, L.R.S., ‎Zago, C.E.S. and Taboga, S.R. (2011).Morphological ‎changes of the epididymis and description of the ‎excurrent ducts of Phrynopsgeoffroanus (Testudines: ‎Chelidae) during the reproductive cycle. Anat. Rec., ‎‎294:145–155 ‎
  7. Cornwall, G.A. (2009). New insights into epididymal biology ‎and function. 400, New York, EUA. Hum.Reprod. ‎Update., 15(2):213-227 ‎
  8. Dacheux, J.L., Castella, S., Gatti, L.J. andDacheux, F. ‎‎(2005).Epididymal cell secretory activities and the role ‎of the proteins in boar sperm epididymis. Theriogenol., ‎‎63(2):319-341 ‎
  9. Djakiew, D. and Jones, R.C. (1982). Ultrastructure of the ‎ductus epididymidis of the echidna, ‎Tachyglossusaculeatus. J. Anat., 135:625–634 ‎
  10. Flesch, F.M. andGadella, B.M. (2000). Dynamics of the ‎mammalian sperm plasm membrane in the process of ‎fertilization. Biochimica et BiophysicaActa ‎‎1469(3):197-235 ‎
  11. Flickinger, C.J. (1983). Synthesis and secretion of glycoprotein ‎by the epidi¬dymal epithelium. J.Androl., 4:157-161 ‎
  12. Gatti, J.L., Castella, S., Dacheux, F., Ecroyd, H., Metayer, S., ‎Thimon, V. andDacheux, J.L. (2004). Post-testicular ‎sperm environment and fertility. AnimReprod Sci., 82-‎‎83:321-339 ‎
  13. Gist, D.H., Dawes, S.M., Terry, W., Turner, T.W., Sheldon, S. ‎and Congdon, J.D. (2001). Sperm Storage in Turtles: A ‎Male Perspective. Expt. Zool., 292:180–186 ‎
  14. Goyal, H.O. and Williams, C.S. (1991). Regional differences in ‎the morphology of the goat epididymis: a light ‎microscopic and ultrastructural study. Am. J. Anat., ‎‎190:349-369 ‎
  15. Hermo, L, Chong, D.L, Moffatt, P., W.S., Waheed, A. and ‎Smith, C.E. (2005). Region and cell – specific differences ‎in the distribution of carbonic anhydrases II, III, XII, ‎and XIV in the adult rat epididymis. ‎J.Histochem.Cytochem. 53(6):699-713 ‎
  16. Hermo, L., Dworkin, J. and Oko, R. (1988). Role of epithelial ‎clear cells of the rat epididymis in the disposal of the ‎contents of cytoplasmic droplets detached from ‎spermatozoa. Am J Anat., 183:107–124 ‎
  17. Hermo, L., Papp, S. andRobaire, B. (1994). Developmental ‎expression of the Yf subunit of glutathione S-transferase ‎P in epithelial cells of the testis, efferent ducts, and ‎epididymis of the rat. Anat. Rec., 239:421–440 ‎
  18. Hermo, L. andRobaire, B. (2002). Epididymis cell types and ‎their function. In The Epididymis: From Molecules to ‎Clinical Practice (B. Robaire and B. T. Hinton, Eds.), ‎Kluwer Academic/Plenum, New York, pp 81–102 ‎
  19. Holmes, H.J. and Gist, D.H. (2004).Excurrent duct system of ‎the male turtle Crysemyspicta. J.Morphol., 261(3):312-‎‎322 ‎
  20. Kellner, A. andSchwanke, C. (2001). Repteis fosseis do Brasil: ‎breve introducao a herpetologia. In: Brito IM. (Ed.). ‎Geologia historica, Uberlandia: Edufu, pp 303-363 ‎
  21. Kirkpatrick, D.T. (1995). An Essay on Taxonomy and the ‎Genus Pelusios. http://www.unc.edu/~dtkirkpa/stuff/pel.‎html. Accessed 22 February 2013.‎
  22. Massanyi, P., Jancova, A. andUhrin, V. (2003). Morphometric ‎study of male reproductive organs in the rodent species ‎ApodemusSylvaticus and Apodemusflavicollis. Bull. Vet. ‎Inst.Pulway.,47:133-138 ‎
  23. Meeran, M.M., Daisy, P. andAkbarsha, M.A. (2001). ‎Histological differentiation along the ductus ‎epididymides of the lizard Calotes versicolorDaudin. J ‎Anim.Morphol. Physiol., 48:85–96 ‎
  24. Oke, B.O., Aire, T.A., Adeyemo, O. and Heath, E. (1987). The ‎structure of the epididymis of the African the giant rat ‎‎(Cricetomysgambianus, Waterhouse). Histochemical ‎and microstereological studies. J. Anat., 160:9-19 ‎
  25. Oke. B.O., Aire, T.A., Adeyemo, O., Heath, E. (1989). The ‎ultrastructure of the epididymis of the African giant rat ‎‎(Cricetomysgambianus, Waterhouse). J Anat., 165:75-‎‎89 ‎
  26. Oke, B.O. andAire, T.A. (1990). Ultrastructural evidence for ‎secretion in different zones of the caput epididymis of ‎the African giant rat ‎‎(Cricetomysgambianus,Waterhouse). Arch. Vet. Med., 60 ‎‎(4):207-212 ‎
  27. Olukole, S.G., Oyeyemi, M.O. andOke, B.O. (2009). ‎Biometrical observations on the testes and epididymis of ‎the domesticated adult African great cane rat ‎‎(Thryonomysswinderianus). Eur. J. Anat., 13(2):71-75‎
  28. Olukole, S.G. andObayemi, T.E. (2010).Histomorphometry of ‎the Testis and Epididymis in the domesticated adult ‎African great cane rat (Thryonomysswinderianus). Int. ‎J.Morphol.,28(4):1251-1254 ‎
  29. Olukole, S.G. andOke, B.O. (2014). Morphological analysis of ‎the plastron of the African sidenck turtle ‎‎(Pelusioscastaneus) Trop. Vet., 32(1&2):26-35 ‎
  30. Olukole,S.G.,Oyeyemi,M.O. andOke,B.O. ‎‎(2014a).Biometricalandhistometrical observationsonthe ‎testisandepididymisof the Africansideneckturtle ‎‎(Pelusioscastaneus). Eur. J. Anat.,18 (2):102-108‎
  31. Olukole,S.G.,Oyeyemi,M.O. andOke,B.O. ‎‎(2014b).Spermatogeniccycleofthe ‎Africansideneckturtle,Pelusioscastaneus(Schweigger,18‎‎12)(Reptilia:Testudines), Ital.J.Zool.,81(1):25-31.‎
  32. Olukole, S.G., Madekurozwa, M-C. and Oke, B.O. (2018). ‎Spermiogenesis in the African sideneck turtle ‎‎(Pelusioscastaneus): Acrosomal vesicle formation and ‎nuclear morphogenesis. J. King Saud Univ. - Science 30: ‎‎359-366‎
  33. Rao,R.J.andShaad, F.U. (1985). Sexual cycle of the male ‎freshwater turtle,Trionyxgangeticus ‎‎(Cuvier)Herpetologica 41(4):433-437 ‎
  34. Rheubert, J.L., Wilson, B.S., Wolf, K.W. andGribbins, K.M. ‎‎(2010a). Ultrastructural study of spermiogenesis in the ‎Jamaican Gray Anole, Anolislineatopus (Reptilia: ‎Polychrotidae). Acta.Zool (Stockholm)., 91:484–494 ‎
  35. Rheubert, J.L., McMahan, C.D., Sever. D.M., Bundy. M.R., ‎Siegel, D.S. andGribbins, K.M. (2010b). Ultrastructure of ‎the reproductive system of the Black Swamp Snake ‎‎(Seminatrixpygaea). VII. Spermatozoon morphology ‎and evolutionary trends of sperm characters in snakes. ‎J. Syst. Zool.Evol.. Res., 48:366–375 ‎
  36. Robaire, B. andHermo, L.(1988). Efferent ducts, epididymis ‎and vas deferens: structure, functions and their ‎regulation. In: The physiology of reproduction, Knobil ‎and J. Neil, (Ed.), Raven Press, New York, pp 999-1080 ‎
  37. Robaire,B., Hinton, B.T. andOrgebin-Crist, M.C. (2006). The ‎Epididymis. In: The physiology of reproduction, Knobil ‎and J. Neil, (Ed.), Elsevier, pp 1071-1148 ‎
  38. Schimmimg, B.C., Pinheiro, P.F.F., Vicentini, C.A. ‎andDomeniconi, R.F. (2012). Ultrastructure of the ‎epithelium lining of cauda epididymidis in mongrel dogs. ‎Pesq. Vet. Bras., 32(1):32-36 ‎
  39. Schimming, B.C. andVicentini, C.A. (2008). Morphological ‎features of the apical region in the principal cells of ‎mongrel dog epididymis. Int. J.Morphol., 26:149-153 ‎
  40. Setchell, B.P., Sanchez-Partida, L.G. andChairussyuhur, A. ‎‎(1993).Epididymal constituents and related substances ‎in the storage of spermatozoa: A review. ‎Reprod.Fert.Devt. 5:601-612 ‎
  41. Sever, D.M. (2009). Ultrastructure of the reproductive system ‎of the black swamp snake (Seminatrixpygaea). Vi ‎Anterior testicular ducts and their nomenclature. ‎J.Morphol., 241:1–12 ‎
  42. Sever, D.M., Rheubert, J.L., Hill, T.A. andSiegal, D.S. (2013). ‎Observations on Variation in the Ultrastructure of the ‎Proximal Testicular Ducts of the Ground Skink, ‎Scincellalateralis (Reptilia: Squamata). J Morphol ‎‎274:429–446 ‎
  43. Sullivan, R. (2004). Male fertility markers, myth or reality. ‎Anim.Reprod. Sci., 82-83 341- 347 ‎
  44. Wyneken, J. (2001). The Anatomy of Sea Turtles. U.S. ‎Department of Commerce NOAA Technical ‎Memorandum NMFS-SEFSC. 470, pp 1-172 ‎
  45. Yanagimachi, R., Kamiguchi, Y., Mikamo, K., Suzuki, F. ‎andYanagimachi, H. (1985). Maturation of ‎spermatozoa in the epididymis of the Chinese hamster. ‎Amer. J. Anat., 172(4)317-330‎