Main Article Content
Abstract
This study investigated the effects of egg yolk and coconut milk-based extenders on the motility and morphology of chilled and post thawed turkey (Meleagris gallopavo) semen in Maiduguri, Nigeria. A total of 112 semen samples were collected from seven matured turkey toms, twice weekly for 2 months using the dorso-abdominal massage method. Freshly collected semen were pooled, divided into three aliquots and diluted with modified Ringer’s buffer, egg yolk and coconut milk-based extenders, respectively. An aliquot of each extended semen was stored at 37°C and 5°C, and then evaluated hourly for 24 hours. Extended semen samples were also cryopreserved in liquid nitrogen and post thaw qualities were assessed after 24 hours. The progressive motility of spermatozoa was maintained for 6 hours at 37°C and up to 12 hours at 5°C when preserved in coconut milk and egg yolk-based extenders. It was also found that use of 1% coconut milk or 1% egg yolk-based extenders improved the post thaw quality of turkey semen once frozen at -196°C. It was observed that the coconut milk-based extender preserved the spermatozoa motility and reduced sperm abnormalities better than the egg yolk-based extender.
Keywords
Modified Ringers, Egg Yolk, Coconut Milk, Cryopreservation, Turkey semen
Article Details
How to Cite
Asuku, S. O., Alkali, I. M., Bukar, M. M., Waziri, M. A., Mustapha, A. R., Ribadu, A. Y., & Amin, J. D. (2021). Effects of Egg Yolk and Coconut Milk-Based Extenders on Chilled and Cryopreserved Turkey (Meleagris gallopavo) Semen Qualities in Maiduguri, Nigeria. Sahel Journal of Veterinary Sciences, 18(4), 29-34. https://doi.org/10.54058/saheljvs.v18i4.258
References
- Aboagla, E. M. and Terada, T.(2003). Trehalose enhanced fluidity of the goat sperm membrane and its protection during freezing. Biology of Reproduction, 69(4): 1245 - 1250.
- Akcay, E., Varisli, O. and Tekin, N.(2006). Fertilizing ability of turkey semen diluted with simple sugar-based extenders after cooled storage.World Poultry Science Association, XII European Poultry Conference, XII European Poultry Conference, Verona, Italy, Pp11 - 13.
- Alkan, S., Baran, A., Ozdas, O. B. and Evecen, M.(2002). Morphological defects in turkey semen.Turkish Journal of Veterinary andAnimal Sciences, 26(5): 1087 - 1092.
- Balogun, A. S., Jimoh, O. A., Olayiwola, T. A. and Abubakar, Z. Y.(2017). Semen quality and fertilizing ability of roosters semen diluted with quail egg-yolk supplemented with polar and non polar dried garlic extracts.Journal of Advances in Biology and Biotechnology, 13(2): 1 - 12.
- Blesbois, E.(2007). Current status in avian semen cryopreservation.World's Poultry Science Journal, 63(2): 213-222
- Burrows, W. H. and Quinn, J. P.(1937). The collection of spermatozoa of domestic fowl and turkey.Poultry Science, 16: 19 - 24.
- Di Iorio, M., Rusco, G., Iampietro, R., Maiuro, L., Schiavone, A., Cerolini, S. and Iaffaldano, N.(2020). Validation of the turkey semen cryopreservation by evaluating the effect of two diluents and the inseminating doses.Animals, 10: 2 - 10.
- Donoghue, A. M. and Wishart, G. J.(2000). Storage of poultry semen.Animal Reproduction Science, 62: 213 - 232.
- Dumpala, P., Parker, H. M. and Mcdaniel, C. D.(2006). The effect of semen storage temperature and diluent type on the sperm quality index of broiler breeder semen.International Journal of Poultry Science, 5(9): 838 - 845.
- Hafez, B. and Hafez, E. S. E.(2000). Reproduction in farm animals, 7th edition (Lippincott Williams and Wilkins, USA), Pp 479-480.
- Iaffaldano, N., Rosato, M. P., Manchisi, A., Centoducati, G. and Meluzzi, A.(2005). Comparison of different extenders on the quality characteristics of turkey semen during storage.Italian Journal of Animal Science, 4(2): 513 - 515. DOI: 10.4081/ijas.2005.2s.513
- Iaffaldano, N., Di Iorio, M., Cerolini, S. and Manchisi, A.(2016). Overview of Turkey Semen Storage: Focus on cryopreservation – A Review:Annals of Animal Science, 16(4): 961-974. doi: https://doi.org/10.1515/aoas-2016-0026
- Kuzlu, M. and Taskin, A.(2017). The effect of different extenders on the sperm motility and viability of frozen turkey semen.Indian Journal of Animal Research, 51(2): 235 – 241. DOI:10.18805/ijar.v0iOF.6825.
- Long, J. A., Purdy, P. H., Zuidberg, K., Hiemstra, S. J., Velleman, S. G. and Woelders, H.(2014). Cryopreservation of turkey semen: effect of breeding line and freezing method on post thaw sperm quality, fertilization and hatching.Cryobiology, 68: 371 - 378.
- Mendes, A. S., de Moura, D. J., Nunes, I. B., dos Santos, I. L., de Souza, C., Morello, G. M. and Takahashi, S. E. (2020). Behavioral responses of turkeys subjected to different climatic conditions.Tropical Animal Health and Production, 52(6): 2855-2862. doi: 10.1007/s11250-019-02106-7
- Mottet, A. and Tempio, G. (2017). Global poultry production: current state and future outlook and challenges. World's Poultry Science Journal, 73(2): 1 - 12.
- Moussa, M., Martinet, V., Trimeche, A., Tainturier, D. and Anton, M.(2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen thawed bull semen.Theriogenology, 57: 1695 - 1706.
- Olurode, S. A. and Ajala, O.(2016). Effects of storage temperature and extension media on motility of caprine spermatozoa.Sokoto Journal of Veterinary Sciences, 14(3): 1 - 8.
- Patil, U., Benjakul, S., Prodpran, T., Senphan, T. and Cheetangdee, N.(2017). A comparative study of the physicochemical properties and emulsion stability of coconut milk at different maturity stages.Italian Journal of Food Science, 29(1): 145 - 157. https://doi.org/10.14674/1120-1770/ijfs.v536
- Santiago-Moreno, J., Bernal, B., Pérez-Cerezales, S., Castaño, C., Toledano-Díaz, A., Esteso, M.C., Gutiérrez-Adán, A., López-Sebastián, A., Gil, M.G., Woelders, H. and Blesbois, E.(2019). Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance, PLoS One. Jan 4;14(1):e0209910. doi: 10.1371/journal.pone.0209910. PMID: 30608977; PMCID: PMC6319765.
- Siudzinska, A. and Lukaszewicz, E.(2008). Effect of semen extender and storage time on sperm morphology of four chicken breeds.Applied Poultry Science Research, 17: 101 - 108.
- Swain, J. E. and Smith, G. D.(2010). Cryoprotectants, In:Chian R.C., Quinn P (Eds.), Fertility Cryopreservation, Cambridge: Cambridge University Press.New York, USA, 24-38. https://doi.org/10.1017/ CBO9780511730207